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Nineteen spots were changed significantly after FA treatment.Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示，甲醛刺激后19个蛋白斑点发生变化，肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点，已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ，磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依靠磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6，这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示，甲醛刺激后19个蛋白斑点发生变化，肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点，已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ，磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依赖磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6，这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

In order to better understand their phylogenetic relationship and identify the uncertain species, the sequences of the small subunit ribosomal RNA (ssurRNA, 16 S rRNA) genes of nine microsporidia infectious to the silkworm, Bombyx mori, were determined.

微孢子虫是真核生物，但其核糖体及核糖体RNA为原核生物型。为探讨9种家蚕病原性微孢子虫的种属地位及亲缘关系，对已广泛用於生物进化分类的核糖体小亚单位RNA基因进行了研究。

OBJECTIVE ①To compare the sequences of the ribosomal rDNA-ITS2, 28S-D3 and mitochondrial mtDNA-COII in the different origins of Anopheles minimus for detecting the intra-and inter-specific variation of the sibling species of the Minimus Complex;②To reconstruct phylogenetic trees of the Minimus Complex and Myzomyia Series for determining the position of An.

目的 ①比较微小按蚊核糖体rDNA-ITS2、28S-D3基因和线粒体mtDNA-COII基因的序列差异，阐明微小按蚊复合体亲缘种种间和种内变异；②根据基因变异重建微小按蚊复合体及迈蚊系成员的种系发生关系，以确立微小按蚊亲缘种变异的种系地位；③建立微小按蚊复合体亲缘种A和C的分子鉴别方法。

OBJECTIVE ①To compare the sequences of the ribosomal rDNA-ITS2, 28S-D3 and mitochondrial mtDNA-COII in the different origins of Anopheles minimus for detecting the intra-and inter-specific variation of the sibling species of the Minimus Complex;②To reconstruct phylogenetic trees of the Minimus Complex and Myzomyia Series for determining the position of An. minimus A and C;③To establish specific methods for differentiation of two sibling species, An. minimus A and An. minimus C, of the Minimus Complex.

中文题名微小按蚊种群变异与分子鉴别研究副题名外文题名论文作者周水森导师汤林华研究员学科专业分子流行病学研究领域\研究方向学位级别博士学位授予单位中国预防医学科学院学位授予日期2002 论文页码总数98页关键词蚊微小按蚊馆藏号BSLW /2003 /R384 /3 目的①比较微小按蚊核糖体rDNA-ITS2、28S-D3基因和线粒体mtDNA-COII基因的序列差异，阐明微小按蚊复合体亲缘种种间和种内变异；②根据基因变异重建微小按蚊复合体及迈蚊系成员的种系发生关系，以确立微小按蚊亲缘种变异的种系地位；③建立微小按蚊复合体亲缘种A和C的分子鉴别方法。

This paper examines the sequence and organization of the ribosomal RNA gene,the ITS region of Nosema antheraeae,and present molecular biological evidence of Nosema antheraeae taxonomy.

目的研究柞蚕微孢子的核糖体基因，转录间隔区以及它们的排列顺序，为柞蚕微孢子的分类和系统进化分析提供分子生物学方面的依据。方法采用特异引物进行PCR扩增，克隆和测序。

Nosema antheraeae ; Ribosomal RNA gene ; ITS ; Organization ; Phylogenetic analysis

柞蚕微孢子；核糖体基因；转录间隔区；排列顺序；系统分析

The sequences of internal transcribed spacer regions of nuclear ribosomal DNA of 9 samples of Nymphaea and related water lilies as outgroups were sequenced and assembled with the sequences in GenBank to analyze the specific and infraspecific relationships.

本文测定了睡莲属9个样品的核核糖体DNA ITS区序列，结合GenBank中获得睡莲类ITS序列，分析了这些样品间的亲缘关系。

Methosd The complete nuclear ribosomal DNA second internal transcribed spacer (ITS2) gene sequence of eggs in sputum from a paragonimiasis patient was obtained by directly auto-sequencing its PCR product .

先从并殖吸虫病患者痰中分离出虫卵，然后PCR扩增出虫卵中完整的核糖体DNA第二间隔区基因(ITS2 )，并直接用于测序从而获得该基因的核苷酸序列。

According to the alignment of Blastn and Blastx, one differentially exp ressed fragment, A7, was 97% identical in nucleotides to the 26S ribosomal RNA gene of Photinia fraseri and 91% in amino acids to cytochrome P450 monooxygenase in maize, respectively. Another differentially exp ressed fragment, A16, was 85% in nucleotides and 93% in amino acids identical to alpha-expansin 2 in Triphysaria versicolor.

核酸和氨基酸比对分析中同源性最高的是片段A7和A16.A7的核苷酸序列同源于红叶石楠Photinia fraseri 26S核糖体RNA (97%)、蛋白同源于玉米的细胞色素P450单加氧酶(91%)， A16的核苷酸(85%)及蛋白(93%)同源于直果草属 Triphysaria versicolor 的α-expansin 2基因。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。