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Results: T cell anergy was antigen-specific. The state of T cell anergy can be reversed by PHA, CD3 mAb and PMA plus A23187. IL-2 can prevent the induction of T cell anergy, but it can not reverse the state of un-responsiveness. IL-2 and IFN mRNA can not express in anergic T cells. In contrast, IL-4 and IL-10 mRNA were detectable.

结果：T细胞无能为抗原特异性的，多克隆激活剂PHA、CD3单抗和PMA+A23187可以恢复无能T细胞的活性；IL-2可以阻止T细胞无能的形成，但不能逆转其耐受状态；无能T细胞IL2和IFN mRNA是关闭的，而IL-4和IL-10 mRNA则是开放的。

An efficient method for analyzing Arbutin and Hydroquinone in cosmetics by reversed-phase high performance liquid chromatography is developed.

探讨了高效液相色谱法同时测定美白化妆品中熊果苷和氢醌含量的方法，先对样品进行预处理，处理液为氯仿-饱和氯化钠溶液(体积比2∶1)，再选用Apollo C18柱，以甲醇-磷酸缓冲液-冰醋酸(体积比25∶475∶1)混合液为流动相，紫外检测，波长为254nm。

METHODS The contents of synephrine,neohesperidin,hesperidin, naringin and narirutin in the raw materials and the extracts of Citrus aurantium were determined by reversed-phase high performance liquid chromatography with an Inertsil ODS-3(4.6 mm×250 mm,5 μm) column, respectively.

方法采用高效液相色谱法，以InertsilODS-3(4.6mm×250mm，5μm)色谱柱分别对枳实及其提取物中辛弗林生物碱类成分和新橙皮苷、橙皮苷、柚皮苷、芸香柚皮苷黄酮类成分进行测定。

The separation of optical isomers of 2, 2'-dihydroxy-1, 1'-binaphthyl and 2, 2'-diamino-1, 1'-binaphthyl was studied by reversed-phase high performance liquid chromatography on a chiral stationary phase consisting of-hydroxypropyl-β-cyclodextrin (Cyclobond Ⅰ 2000 SP)using methanol/water as the mobile phase. The effects of mobile phase ratio, flow rate and column temperature on separation of optical isomers of DHBN and DABN were investigated.

在S-羟丙基－β-环糊精手性柱(CyclobondⅠ2000 SP)上用反相高效液相色谱法研究了2，2'-二氨基－1，1'-联萘和2，2'-二羟基－1，1'-联萘光学异构体的分离，考察了流动相组成、流速以及柱温等对联二萘胺和联二萘酚光学异构体分离的影响。

First of all,appeal the law to prohibit reversed payment,unify this with the BOLAR exception,and promote the drugs into the market as soon as possible.

对我国解决公共健康危机的国内法律策略进行探析，提出八种解决措施：强制许可、平行进口、专利权的例外、对限制竞争行为的控制、BOLAR例外、禁止反向支付、对传统知识和遗传资源予以知识产权保护以及医药专利审查制度——&事先同意&法律制度。

This paper proposes our country eight kinds of legal strategies. They are compulsory licensing, parallel import, the exception of patent protection, to control the limiting competition behaviors, the BOLAR exception, to prohibit reversed payment, to give the traditional knowledge and the genetic resources IPRs protection and the legal mechanism of Prior Consent.

对我国解决公共健康危机的国内法律策略进行探析，提出八种解决措施：强制许可、平行进口、专利权的例外、对限制竞争行为的控制、BOLAR例外、禁止反向支付、对传统知识和遗传资源予以知识产权保护以及医药专利审查制度―&事先同意&法律制度。

The latter three strategies are new and also the paper's three innovative points. First of all, appeal the law to prohibit reversed payment, unify this with the BOLAR exception, and promote the drugs into the market as soon as possible. Secondly, put the public health, medicine patent and the IPRs protection of traditional knowledge and the genetic resources together. Through giving the traditional knowledge and the genetic resources IPRs protection to treat the developed countries' medicine patents, they can settle the public health crisis. Finally, the legal mechanism of Prior Consent is a new strategy that Brazil proposed for the purpose of protecting the public health, which divides the examination of patent applications for pharmaceutical products and processes between two federal agencies, National Institute of Intellectual Property and National Sanitary Supervision Agency. ANVISA has the obligation to render the final decision, granting or denying a patent.

其中前四种措施是国际社会上一直以来倡导的主要措施，后三种是新的解决公共健康危机的法律措施，其创新点在于：第一，呼吁将禁止反向支付纳入法律，并与BOLAR例外相结合使用，促进仿制药品的尽快上市；第二，将公共健康、医药品专利以及传统知识和遗传资源的知识产权保护三者紧密相连，通过对传统知识和遗传资源予以知识产权保护来应对发达国家的医药品专利；第三，&事先同意&法律制度是巴西提出的一种旨在保护公共健康，应对医药专利的新的法律制度，该制度规定凡是涉及医药品专利的申请都必须经过国家知识产权局和国家公共卫生监督局的公共审查，并且国家公共卫生监督局有是否授予专利的最终决定权。

P. O. Dolphin's Barn: the transliterated name and address of the addresser of the 3 letters in reversed alphabetic boustrophedonic punctated quadrilinear cryptogram N.

从伦敦市内西区查林十字路邮政局三十二号信箱邮购来的。

Results: Closed rupture of the achilles tendon was often misdiagnosed and failed to be diagnosed clinically due to unsufficient recognition and carelessness about the disease and careless special physical check-up. Among many therapeutic methods for the disease, Bunnell's suture with extractable steel wire was more effective than bending-knee plantar flexion plaster stone fining, direct suturing, auto-fascial strap mending or reversed muscular flap repairing.

结果：缺乏对此病的认识和警惕性，未进行仔细的专科体检，是误诊、漏诊的主要原因；治疗方法较多，其中采取Bunnell氏抽出钢丝缝合术治疗的15例，较屈膝跖屈石膏固定、直接缝合术、自体筋膜条修补术和肌膜瓣翻转修补术治疗效果好。

A determination method of ursolic acid in Callicarpa macrophylla Vahl leaves by reversed- phase high performance liquid chromtograph was established. The chromatographic column (Symmetry Shield RP18), methanol-water mobile phase(88:12, V/V) with 1.0 ml/min flow rate, the detection wavelength (210nm) and the column temperature (25℃) were adopted.

建立反相高效液相色谱测定其含量的方法，色谱柱为Symmetry Shield RP18柱，流动相为甲醇-水（体积比88∶12），流速1.0 ml/min，检测波长210 nm，柱温25℃。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。