查询词典 retroverse

Methods Real-time quantitative retroverse polymerase chain reaction method was used for detecting RbAp46 expression levels in BM cells of 140 patients with acute leukemia,13 with chronic myelogenous leukemia in chronic phase, 7 with CML in blast crisis and 32 with non-leukemic disorders.

方法建立实时定量RTPCR方法，利用SYBRGreenⅠ染料检测140例急性白血病、13例慢性粒细胞白血病慢性期和7例CML急变期患者以及32例非白血病患者骨髓细胞RbAp46基因的表达水平。

Rapid amplification of cDNA end:Retroverse transcription product of total RNA extracted from normal porcine tissue was used as the template,gene specific primers were designed and advantage 2 polymerase mix was used in PCR,of which using porcine genomic DNA as the template:forward primer was designed according to the acquired consensus region of human and pig FGL2 3′ sequences while reverse primer was designed from human FGL2 3′ end downstream sequence;TA cloning.

以猪正常小肠及心脏组织提取新鲜总RNA，反转录后作为模板，设计基因特异性引物，采用Advantage 2 聚合酶混合物进行PCR扩增；依据猪与人FGL2基因3′端已知同源序列设计PCR上游引物，以人FGL2基因3′末端序列设计下游引物，以猪基因组DNA为模板采用Advantage 2 聚合酶混合物进行PCR反应；PCR载体重组质粒DNA亚克隆扩增。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。