查询词典 restriction

The primers and the detection programs have been used to detect the pathogens in the inoculated-diseased cucumber plants .The details are reported as follows: 1. By using the primers ITS1/ITS4, only Fusarium isolates could be amplified to produce a special fragment of 750bp. This product generated 480bp, 200bp and 100bp fragments after digested by restriction enzyme HinfⅠonly from the pathogen of Cucurbits Fusarium Wilt.

引物ITS1/ITS4只在镰孢属菌株上扩增到一条750bp分子片段，其他供试菌株无扩增条带产生，扩增产物经HinfⅠ酶解后，只有瓜类尖镰孢菌产生480bp、200bp和100bp 3条带，说明该PCR-RFLP程序对瓜类尖镰孢菌具有特异性；巢式PCR引物BIK1/BIK4和BIK2/BIK3只有在黄瓜尖镰孢菌菌株上扩增到一条800bp分子片段，其他菌株均无扩增条带产生，说明该巢式PCR程序对黄瓜尖镰孢菌具有特异性。

Screening and identification: the resulting plasmid was extracted by alkali division, identified by restriction enzyme digestions and PCR, observed by 1% agarose gels electrophoresis.

为了提高了目的片段和载体之间的连接效率以及目的片段的拷贝数，本实验所选用的载体是pMD18-T Vector，它是由pUC18用EcoRⅤ切割后在两个3'末端加上单个的碱基'T'而制得的线状分子。

PUC19 plasmid were successfully enriched and purified from the E. coli DH5α under the magnetic fields. The obtained plasmid retained native the bioactivity to be used for restriction enzyme digestions and cell transformations.

用这种方法成功地从大肠杆菌DH5α浓缩和纯化得到了pUC19质粒，该质粒具有生物活性，可直接用于限制性酶切和细胞转化等分子生物学下游操作。

Several dioramas were presented as well, since there was no restriction on the method of presenting the models.

提出了若干西洋镜以及，因为没有任何限制的方法，介绍了模型。

Sodium restriction and diuretics have no role in therapy.Restricted physical activity can lower blood pressure.

钠盐限制和利尿剂对治疗帮助不大，体力活动的限制可以降低血压。

Other Northstar firsts on the XLR include four-cam continuously variable valve timing; electronically controlled, hydraulically actuated intake and exhaust cam phasers on all four camshafts for extra control; electronic throttle control; low restriction intake and exhaust manifolds and cylinder head ports; close coupled catalytic converters mounted directly adjacent to the exhaust manifolds; and a new air induction system, redesigned for enhanced capacity and noise attenuation.

Other北极星上首创的XLR包括四凸轮连续可变气门正时，电子控制，液压驱动进排气上的额外控制所有四个凸轮轴凸轮相位调节；电子节气门控制；低，限制进气和排气管和缸盖港口；紧密耦合催化转化器直接安装毗邻的排气管，以及一个新的空气感应系统，增强能力和噪声衰减重新设计。

If peasants are in a heavy pressure from capital restriction, they may have to sell at the "three sale fastigiums" and lose their opportunities of maximizing their sale profits.

回归结果在整体上验证了本文的整体分析及具体假说，农户的农作物品种越特殊、农户家庭经济中农业比重越高、户主信息可得性越强，农户越倾向于选择&新型销售方式&；农户面临的资金约束是农户选择在&三大销售高峰期&销售的最重要原因，当农户的资金压力形成硬约束时，农户没有选择实现利润最大化的销售时机的自由。

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

At present, the retrofit options include replacing the float valve tower with packed column and increasing the height and diameter of the tower. But it required large investment and might face the restriction of workshop layout.

现有的改造方法是将浮阀塔改为填料塔，增加原塔高和塔径，但存在改造工作量大，费用高，有时会受现场条件的限制等缺点。

Hou uses a retrospective and reflective attitude in making this movie, scripting this historical incident to happen to a normal family, so that the story can be dealt with more rationally. On the other hand, he purposely posed Wenqing, the protagonist in the movie, as a mute. The protagonist being a mute seems to bring about a great restriction to the development of the story, but from another point of view, it actually maximizes the artistic potential of the movie. Visual footages were being presented continuously, and with the various incomplete images being pieced up gradually, it presents Wenqing's world to the viewers in an even clearer light.

导演以一种追忆和反思的姿态面对摄影机介入在已经流逝的时空里演绎一段历史事件在台湾平民家族所发生的故事以一种更为理性的态度去面对和思考电影本身的含义，却偏偏安排了文清，一个哑巴作为整个故事的主角，将电影化的手法扩大到最大，不能说话让故事的进行产生了很大的限制，在另一个角度来看却是将艺术的能量最大空间的给予了发挥的可能性，视觉资源被不断地挖掘，给了看的内容和条件，让人事物的原初画面在断裂的片断中慢慢的连接起来，残象贯穿正是让文清的世界变得更加的明朗化。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。