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This clause applies in addition to and shall not be construed as derogating from any defence or exclusion, restriction or limitation of liability available to the Carrier under the terms of this Bill of Lading or otherwise.

本条款是对减轻和限制运送人根据本提单条款和其它规定应担责任的补充，而不应被视为取消责任的减轻和限制。

The crude DNA pellets were recovered by dialyzing and then could be used for molecular operation such as amplification of 16S rRNA gene and digestion of restriction enzymes.The plasmid pUC19 was prepared and digested with BamHI.

粗提DNA用半透膜透析回收纯化，纯化后的DNA可以进行16sRNA基因PCR扩增，酶切等分子生物学操作，具有较高的质量。

3And the disentangling of state power. After the declaring of the state of emergency,the exercise of state power is' not unlimited,though it can exceed the constitution restriction to some degrees.

在国家宣布紧急状态后，虽然可以根据应对紧急情况的现实需要而采取各种紧急措施，但是这种紧急权力的行使不能是毫无限制的，否则紧急状态的宣布就意味着独裁专制的开始。

The P32 gene of capripoxvirus were amplified by PCR.The product of PCR was cloned to the plasmid pGEM-T-Easy.Then the P32 gene was disparted to three parts.Three parts of P32 gene were inserted into the expressed plasmids pGEX-4T-1, which were identified by PCR and restriction enzyme analysis.

本研究利用PCR方法对羊痘病毒P32蛋白基因扩增，将回收PCR产物克隆到pGEM-T-Easy载体中，然后将P32蛋白基因分成3段，分别插入原核表达载体pGEX-4T-1中，经PCR和酶切鉴定，证明成功构建了重组表达载体pGEX-4T-1-N2/3、pGEX-4T-1-N1/3、pGEX-4T-1-M。

There are many reasons including macroscopic and microcosmic for thatcross-media management of our publishing industry is backward at present. Theseinclude the multi-management system of our media, barriers of the industry entering,restriction of capital borrowing mode, lack of the talents of cross-media management,pretty high profit of the closing publishing industry and the draggles of the employee' idea,etc.

我国出版业跨媒体经营现状落后，既有宏观方面的原因也有微观方面的原因，这主要包括我国各种媒体的多头管理体制，行业进入壁垒，融资渠道的限制，跨媒体经营管理人才的缺乏，出版业行业封闭利润相对较高以及从业人员观念的落后等各方面的原因。

Methods The total RNA was acquired from young rabbit particular cartilage, the BMP7 gene was inserted into pcDNA-3.1 to construct eukaryotic expression vector plasmid of pcDNA3.1-BMP7; the target gene-BMP7 was identified respectively by PCR analysis, restriction enounces analysis and nucleotide sequencing; the plasmid was transfected into adult rabbit knee articular chondrocytes, then the expression of BMP7 was detected by in situ hybridization, PCR and Western blotting.

从体外培养的幼兔膝关节软骨细胞中提取总RNA；按GenBank BMP7基因序列化学合成2条引物，采用RT-PCR方法得到BMP7基因；将BMP7基因片段插入到真核表达载体pcDNA 3.1中，构建pcDNA3.1-BMP7真核表达载体质粒；利用双酶切、PCR及核苷酸序列分析鉴定BMP7目的基因；将重组pcDNA3.1-BMP7真核表达载体质粒转染家兔关节软骨细胞，分别采用原位杂交、PCR、Western blotting法测定BMP-7表达。

This paper brings forward some advice on how to develop agricultural logistics after enumerating the factors of restriction and the conditions of existence.

在列举发展农业物流的约束因素和发展农业物流的现有条件后，提出了发展农业物流的建议。

This paper brings forward some advice on how to develop agricultural logistics after enumerating the factors of restriction and the conditions of existenc...

在列举发展农业物流的约束因素和发展农业物流的现有条件后，提出了发展农业物流的建议。

Firstly, this part exposes the basic theories of the restriction of trademark right.

首先，文中阐述了商标权限制的理论基础。

Though the bill orders the Department of Health and Human Services to negotiate on prices, it does not lift another legal restriction against using preferred drug lists -- called formularies -- at the federal level in the Part D program.

虽然法案命令健康与公共事业部商议价格，但并不影响其它处方药物一览表中的药物。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。