查询词典 restriction

In this study, the structure sequences of Shiga toxin 2B(stx2B) and Shiga toxin 1B(stx1B) were respectively amplified from EHEC O157:H7 by PCR. stx2B-L-stx1B fusion gene was constructed by a 12 bp linker ligating these two fragments. After digested with restriction endonuclease Nco Ⅰ and EcoR Ⅰ, the fusion gene was orientally inserted into expression vector pET28a.

利用PCR方法，从肠出血性大肠杆菌O157：H7的基因组DNA中，分别扩增出Stx1B和Stx2B亚基的结构基因片段，然后再通过PCR将这2个片段连接起来，并定向克隆到表达质粒pET28a的NcoⅠ和EcoRⅠ位点之间，构建了重组表达质粒pMB1。

The otological, audiological and neurological examinations of these patients were conducted, including otoscopy, pure-tone (Madsen 522) and immittance(GSI 33) audiometry. MtDNA A1555G mutation and GJB2 235delC mutation were detected with specific restriction enzyme digestion methods.

对 148 名非综合征型感音神经性耳聋患者分别进行线粒体 DNA 12SrRNA基因A1555G 点突变和 GJB2 基因235delC突变的限制性内切酶分析。

However, during borehole grouting pile construction of the present, round section piles are used universally. Generally speaking, the boreholes are drilled by a large diameter round piling bit. Because of the restriction of factors of the mud pumps, and drilling equipments, and drilling technique, it would result in such problems as fol lowing: a bigger cutting area and overlarge cuttings volume, and more hole bottom sediments, difficult cuttings discharging, and serverely repeat cuttings breaking, and using more concret grouting amount, and lower drilling speed, faster bit wearing, and longer working period, bigger material and labor ex haust, and higher engineering cost, unreasonable ratio of loading capacity to volume of pile foundation, and lower comprehensive economic benefits, and so forth.

然而，钻孔灌注桩施工中，目前最为普遍采用的是圆形断面桩基，一般采用大口径圆形基桩工程钻头，由于国内生产的泥浆泵、钻具及钻进方法等因素的限制，从而使钻孔破岩面积和体积过大，造成孔底沉渣过多，排渣困难且排浆量大，重复破碎问题突出，混凝土灌注量大，直接反映为钻进效率低、钻头磨损快、施工工期长、材料能耗和劳动消耗大、工程成本较高、桩基荷载能力与体积比不合理、综合经济效益不理想等，这些问题严重影响和制约着钻孔灌注桩施工技术的发展和进步。

Methods 1.3 fold overlength genome of HBV,from pHBV-dimer carring two head to tail copies of HBV genome,was cloned into pCR2.1 by combined PCR and restriction enzyme digestion.

目的 构建乙型肝炎病毒感染性复制子表达载体，建立HBV体外复制细胞模型，分析其复制特性及对抗病毒药物的敏感性。

Sticky ends -- Descriptive of the structure of a double-stranded polynucleotide in which one strand extends further from the end than the other to create a single-stranded 'tail'; especially the product of cleavage by a restriction endonuclease at a palindromic sequence.

转移核糖核酸--核糖核酸的一种，当蛋白质合成时，当氨基酸与信使核糖核酸的转接器。

Since Watson and Crick have brought forward the model of DNA helix structure and explain about it's reproduce mechanism in molecule level, research work concerning DNA has been continuously lucubrated. However, whether translating inheritance information and rebuilding DNA molecule via menstruating DNA sequence, or to date the most compelling gene project, chromosome protract et. al, there all need a sort of molecule scissors cleaving DNA, DNA cleavage reagent. At present, tool enzyme of cleaving DNA is mostly DNA restriction enzymes founded in 70 years, which only cleave DNA at specific 4-8 base pair sequences that are usually palindromic, so this restricts the at very fast speed developing of gene technology at certain extent.

自从Watson和Crick提出DNA的双螺旋结构并对其复制机制在分子水平上进行解释后，使得对DNA的研究不断深入，但无论是通过测定DNA的序列来破译遗传信息、改造DNA分子，还是目前最令人关注的基因工程、染色体绘制等，都需要一种断裂DNA分子的&剪刀&——DNA断裂试剂，目前断裂DNA的工具酶主要是上个世纪70年代发现的DNA限制性内切酶，但所知的Ⅱ型限制性内切酶的DNA序列识别长度较短（约为4～8个碱基对），而且一般要求回文结构，这就给飞速发展的基因工程技术带来一定的限制。

Sticky ends -- De script ive of the structure of a double-stranded polynucleotide in which one strand extends further from the end than the other to create a single-stranded 'tail'; especially the product of cleavage by a restriction endonuclease at a palindromic sequence.

转移核糖核酸--核糖核酸的一种，当蛋白质合成时，当氨基酸与信使核糖核酸的转接器。

In this paper, problems of trend test in clinical experiment are studied mainly by the statistical inference method under order restriction. The ori- gins of order restricted statistical inference are usually dated back to the early 1950s. At about the same time, a number of researchers started working inde- pendently on similar problems. The field developed rapidly during the 1960s and early 1970s and formed the more perfect theory of estimation and test- ing.

中文题名临床试验中的趋势性检验副题名外文题名 Trend test in clinical experiment 论文作者马艳萍导师史宁中教授学科专业概率论与数理统计研究领域\研究方向学位级别博士学位授予单位吉林大学学位授予日期2002 论文页码总数97页关键词临床试验趋势性检验数理统计约束下统计推断馆藏号BSLW /2003 /R311 /1 本文主要利用序约束下的统计推断方法对临床试验中的趋势性检验问题进行了研究。

The development of military affairs technic of Taiwan is special, the special political requirement inside and the complex international relation make its , ilitary by thought of "Taiwan independence", So its military affairs technic is restricted by the need of weapons for a long time, and badly depend on the environment, Especially restricted by Aamerica, which make a kind of deviant need draught and dependency, Because of restriction its politics, economic strength, sources and international environment, Taiwan cannot sustain a perdure war, to improve the short time battle effectiveness because the first aim of military affairs technic has many shortcoming.

由于台湾自身政治、经济实力、能源资源以及国际环境的局限，台湾并不能够承担持久战，所以提高短期战斗力成为台湾军事技术发展的中心目标，而其军事技术发展的直接目标表现为武器获得。

Phasianus ; Chrysolopus ; Mitochondrial DNA ; Restriction map ; Molecular phylogenetic tree

雉鸡；锦鸡属；线粒休DNA ；限制性内切酶图谱；分子系统树

Just so you know where we're going...

只要让你知道我们的目标。。。

One of his friends acted as the gobetween in his business.

在这件事中，他的一个朋友充当了中间人。