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Then pMD-P80 is digested by the enzymes Xho I and Apa I, separated and linked to the linear plasmid pEGPF-C1 after digested by Xho I and Apa I. So the recombined expression plasmid pGFP-P80 is achieved. Then it is sequenced, PCR and digested by the restriction enzymes Xho I and Apa I. The results give that pGFP-P80 is achieved successfully and the insert sites, direction and reading frame are all right. It builds the basis for expressing, purifying, gaining p80 protein from mammiferous cells.

将pMD-P80 分别经Xho I 和Apa I 双酶切和回收，然后与经过Xho I 和Apa I 酶解的真核表达载体pEGPF-C1 连接、转化，获得重组质粒，经PCR，Xho I 和Apa I 限制性酶切和序列测定，鉴定为真核表达质粒pEGFP-P80，并且目的基因的插入位置、方向和读码框完全正确，为在哺乳动物细胞中表达并分离纯化p80 蛋白奠定了基础。

Institutionalized management Taking part in in the work of establishing and improving managemental institution poses an efficacious restriction to the entire collaborations and the respective act...

加强同各机构之间、岗位之间的联系，在从宏观上统一思想的基础上，严格依照组织所面临的内外部环境确立和变更管理制度体系

I have taken part in the work of establishing and improving managemental institution that would pose an efficacious restriction to the entire collaborations and the respective actions with the science means and rational authorities.

参与建立健全企业内部管理制度的工作，以科学为手段，以理性为权威对生产协作及员工行为进行规范有效的管理。

In this research, we consider that the predominant bacteria in low temperature wastewater treatment in winter were cold-adapted microorganisms, the number of which increased as the temperature dropped more and more, but rarely high up to the number of mesophilic bacteria in normal temperature due to its low growth rate and the restriction of ecological factors, so that the total number and activity level of microorganism in activated sludge decreased.

随着污水温度的降低，污泥中冷适应微生物的数量逐渐增加，但由于冷适应微生物的生长速率相对较低，且容易受到各种生态因子的抑制作用，其数量无法达到常温下中温微生物的水平，使得污泥中微生物的总体数量和活性降低。

The patients with methylmalonic acidemia and homocystinuria should be mainly treated with vitamin B12, betaine and L-carnitine, while the patients with isolated methymalonic acidemia should be treated with protein restriction, the special milk formula without isoleucine, valine, methionine, and threonine, and be given L-carnitine.

对伴有同型半胱氨酸血症患儿，治疗以维生素B12、甜菜碱和左旋肉碱为主；对不伴有同型半胱氨酸血症患儿以限制天然蛋白质摄入，给予去除异亮氨酸、缬氨酸、甲硫氨酸和苏氨酸的特殊奶粉及左旋肉碱治疗为主。

Positioning is examined by using micrococcal nuclease to examine cleavage sites relative to a restriction site at the 5 end of the gene.

最初基因会展现一个核小体的模型，它是由穿过基因一段有效距离的启动子构成的；在3ˊ端范围中定位作用遗失。

Different genotypes were determined in Luxi monotocous cows, Luxi twinning cows, Chinese Simmental cows, Angus cows and Simmental×Mongolia cows by restriction fragment length polymorphism. The value of polymorphism information content indicated that this was a moderate polymorphism in Luxi monotocous cows and Luxi twinning cows.

运用PCR-RFLP法验证并分析该位点在鲁西牛双胎群体和单胎群体及中国西门塔尔牛、安格斯牛和西蒙杂交牛单胎群体间的多态性，结果表明，在鲁西牛双胎和单胎群体中分布A、B两个等位基因，处于中度多态。

The compulsive restriction by government power and the moralization of intellectuals often worked together.

官府和士绅在改革中往往是交互作用，国家力量的强制约束同士绅的道德教化重叠起作用。

Des Moul., Ajania Poljakov and their allies are still unresolved due to the restriction of plant materials and complicated geographic distribution.

由于研究材料、地理分布等条件的限制，菊属、亚菊属及其近缘属的属间关系未得到很好解决。

Digested by restriction endonuclease,and cloned HPC2 into mutic...

人HPC2基因的原核及真核表达载体的构建为深入研究HPC2基因与宫颈癌的发生奠定了基础。

As of Tuesday, Google's results were still censored in China.

截至周二，谷歌的搜索结果仍受中国审查。

In order to make the positive action increase and negative one decrease, the sub-forces of the social factors must be adjusted to form a centripetal force.

在这一过程中，人的主体性发挥是社会有机体健康发展的灵魂。