查询词典 restriction

Methods The target cDNA was amplified by PCR and PCR products were indigested by restriction enzyme Nco Ⅰ and Hind Ⅲ. Then the indigested products were recombined into pET-BBH and pET-BBH was expressed in E. coli BL21 (DE3) by IPTG inducing. Next, the expressed proteins were identified by SDS-PAGE, western blot and enzyme activity test. Finally, fluorescence quantitative PCR was used to test its expression quantity in different worm stages.

PCR扩增GAMMA-BBH cDNA基因，产物经NcoⅠ和HindⅢ限制性内切酶酶切后连接至原核表达载体重组为pET-BBH，在BL21(DE3)中用IPTG诱导表达，SDS-PAGE、Western blot和酶活性测定鉴定表达产物，用荧光定量PCR方法检测广州管圆线虫不同虫龄GAMMA-BBH的表达量。

The durable goods, which is different from indurable goods, the consumption have some relation with budget restriction, and the previous consumption because they can be used for many period.

当前对消费理论和实证的研究是一个热点，而耐用品消费构成家庭消费的一个重要组成部分，作为耐用商品与非耐用商品消费不同的地方是，它不仅与预算约束有关，同时由于它可以多个时期消费，因此也与以前的消费有关。

Results: Identification of the Ad-pml by restriction digestion and PCR showed the length. Ad-pml was confirmed in sequence. fluorescence showed successful recombined adenovirus with infectiveness.

结果：经PCR反应可扩增出1767bp大小的片段，测序结果检测Ad-pml序列正确，通过免疫荧光反应证明病毒包装成功并且具有感染力。

The objectives include compliance with applicable laws and regulations, reliability of information, realization of enterprise's operating objective, controlling of risks, rationality of inspiriting and restriction.

企业内部控制的具体目标由法规遵守性、信息的真实性、经营目标的实现、风险控制、激励约束机制的合理性等内容构成。

Meanwhile, it is also illustrates that the smaller chromium carbides have less restriction to forming thin splats than larger ones, which beneficial to limit the size of pieces removed by impact of the sand particles, and the higher volume fraction of small chromium carbide may effect the erosion through enhancing interlamellar bonding of the splats which leads to increase the erosion resistance of the coating.

揭示了细小碳化物颗粒有利于获得扁平粒子厚度较小的涂层结构，可减小涂层冲蚀磨损过程中每次剥落的体积；较高含量的细小碳化物颗粒有利于提高涂层扁平粒子间的结合，从而提高涂层抗冲蚀磨损性能。

For interlingual sources,the thesis lays emphasis on NL interference.And for intralingual sources,it focuses on overgeneralization,ignorance of rule restriction,false concepts hypothesized and incomplete application of rules. Accordingly,relevant error correction methods and pedagogical suggestions are put forward in order to be helpful to teaching strategies,teaching focal points and instruction of writing skills and further to increase the writing ability of the learners.

在语际因素方面，母语的负迁移问题是讨论的主要内容；而在语内因素方面，主要就过度概括，忽视规则错误，规则的不完全应用和错误概念假设进行了讨论，并据此提出了相应的改正措施，希望对以后的写作教学策略、教学侧重点及写作技巧指导等方面有所帮助，进而帮助学习者提高写作能力。

Several factors increase the risk of a first hemorrhage: a small malformation, exclusively deep venous drainage (both of which are easily detected by brain imaging and cerebral angiography), and high intranidal pressure, which is reflected by high pressures in the feeding arteries or restriction of venous outflow.

增加的首次出血风险的几个因素：小的血管畸形，完全是深静脉引流（这两者都很轻易通过脑成像与脑血管造影检测）；高颅压，是由于供血动脉压力增高或静脉引流不足造成。

After explained that attention "bottleneck" had exerted a restriction on information obtaining in information receiving process, this paper brings forward a viewpoint that information set is decided by attention namely a subjective resource can deviate investors' expectation from the real, and blurs action set and state set, then irrationalize economic activities.

分析了信息接收过程中，注意力&瓶颈&对信息获取施加的约束，提出信息集的注意力主观资源决定性使决策者预期偏差化，行动空间和状态空间模糊化，进而使经济行为非理性化的观点。

Therefore, the restriction of humanity is not justificatory, but conditional, only if humanitarian ideal is dominant o-ver the penalty idea, humanity will restrict penalties.

因此，人道对刑罚的限制不是根据性的，而是条件性的，只有当人道理念较比刑罚理念占优势时，人道才限制刑罚。

Methods 202 kinless subjects of the Chinese Han were investigated in this study, including 82 normal controls, and 120 cases diagnosed as CHD. The PPARδ+294T/C gene polymorphism was determined by polymerase chain reaction and restriction fragment length polymorphisms. The frequencies of PPARδ+294T/C genotypes and the "C"allele frequency were analyzed, to evaluate the risk for the CHD among variant genotypes.

运用聚合酶链式反应及限制酶片段长度多态性技术分析无血缘关系汉族人群[包括82例正常对照者，120 例冠心病患者]的PPARδ+294T/C基因多态性，分析基因型频率、等位基因频率，并对不同基因型患者冠心病的危险性进行评价。

As of Tuesday, Google's results were still censored in China.

截至周二，谷歌的搜索结果仍受中国审查。

In order to make the positive action increase and negative one decrease, the sub-forces of the social factors must be adjusted to form a centripetal force.

在这一过程中，人的主体性发挥是社会有机体健康发展的灵魂。