查询词典 restriction

After the NK gene from recombinant plasmid pUC19-NK was cloned on expression vector pBV220, it was transformed into host cell E. coli HB101. Recombinant plasmid pBV220-NK was extracted for identifying by analysis of restriction enzymes, PCR and sequencing. Studies on recombinant strain's growth and expression showed that heterogene have no distinct effect on host cell. The recombinant plasmid have excellent segregational stability but low structural stability. The results of kinetics of pBV220-NK expression in E.

将重组质粒pUC19-NK上的纳豆激酶基因成功克隆到表达载体pBV220上，并将之转入大肠杆菌HB101中，得到转纳豆激酶基因工程菌，提取重组质粒经单酶切、双酶切及PCR分析验证后，对重组菌的生长及表达进行一系列研究，结果表明：外源纳豆激酶基因的导入对宿主的生长没有明显的影响：重组质粒的稳定性实验表明：该质粒具有良好的分离稳定性，而结构稳定性较差。

In this program, the declining segment of the stress-strain relation of the concrete, the restriction effect of hooped reinforcement and the bond-slip problem of the reinforcement are considered, as the same time the optimized arithmetic is applied to accelerate calculation procedure. Though the appropriate experiment arrangement, the influential factor such as the strength of concrete, the ratio of hooped reinforcement, the ratio of axial compression and the loading angle are researched, some available rules of carrying ability and ductility of the special-shaped column can be attained.

程序中考虑了混凝土应力应变曲线的下降段及箍筋对混凝土的约束影响，采用了合适的算法加速计算过程并考虑了纵筋锚固滑移的影响，通过正交试验分析了混凝土强度、体积配箍率、轴压比和荷载角对异形柱截面抗弯承载能力及截面曲率延性比的影响。

Experiments show that this method overcomes the historical hydrographic data precision dependency and SAR image condition restriction of the traditional methods.

实验表明，该方法克服了现有方法依赖于历史水文数据精度和SAR成像条件的限制，其反演结果与实测值十分接近。

The thesis advances a new optimality design method which can consider stress and proper frequency restriction condition synthetically, through basing on stress optimality criteria methods, owing to optimality criteria don't relate to multi-constraint and apply incomprehensively.

本文针对优化准则法不能考虑多种优化约束条件、通用性差的缺点，基于应力优化准则法和固有频率优化准则法，提出了一种可以综合考虑设计模型的应力约束条件和固有频率约束条件的优化设计方法。

Methods The target cDNA was amplified by PCR and PCR products were indigested by restriction enzyme Nco Ⅰ and Hind Ⅲ. Then the indigested products were recombined into pET-BBH and pET-BBH was expressed in E. coli BL21 (DE3) by IPTG inducing. Next, the expressed proteins were identified by SDS-PAGE, western blot and enzyme activity test. Finally, fluorescence quantitative PCR was used to test its expression quantity in different worm stages.

PCR扩增GAMMA-BBH cDNA基因，产物经NcoⅠ和HindⅢ限制性内切酶酶切后连接至原核表达载体重组为pET-BBH，在BL21(DE3)中用IPTG诱导表达，SDS-PAGE、Western blot和酶活性测定鉴定表达产物，用荧光定量PCR方法检测广州管圆线虫不同虫龄GAMMA-BBH的表达量。

The durable goods, which is different from indurable goods, the consumption have some relation with budget restriction, and the previous consumption because they can be used for many period.

当前对消费理论和实证的研究是一个热点，而耐用品消费构成家庭消费的一个重要组成部分，作为耐用商品与非耐用商品消费不同的地方是，它不仅与预算约束有关，同时由于它可以多个时期消费，因此也与以前的消费有关。

Results: Identification of the Ad-pml by restriction digestion and PCR showed the length. Ad-pml was confirmed in sequence. fluorescence showed successful recombined adenovirus with infectiveness.

结果：经PCR反应可扩增出1767bp大小的片段，测序结果检测Ad-pml序列正确，通过免疫荧光反应证明病毒包装成功并且具有感染力。

The objectives include compliance with applicable laws and regulations, reliability of information, realization of enterprise's operating objective, controlling of risks, rationality of inspiriting and restriction.

企业内部控制的具体目标由法规遵守性、信息的真实性、经营目标的实现、风险控制、激励约束机制的合理性等内容构成。

Meanwhile, it is also illustrates that the smaller chromium carbides have less restriction to forming thin splats than larger ones, which beneficial to limit the size of pieces removed by impact of the sand particles, and the higher volume fraction of small chromium carbide may effect the erosion through enhancing interlamellar bonding of the splats which leads to increase the erosion resistance of the coating.

揭示了细小碳化物颗粒有利于获得扁平粒子厚度较小的涂层结构，可减小涂层冲蚀磨损过程中每次剥落的体积；较高含量的细小碳化物颗粒有利于提高涂层扁平粒子间的结合，从而提高涂层抗冲蚀磨损性能。

For interlingual sources,the thesis lays emphasis on NL interference.And for intralingual sources,it focuses on overgeneralization,ignorance of rule restriction,false concepts hypothesized and incomplete application of rules. Accordingly,relevant error correction methods and pedagogical suggestions are put forward in order to be helpful to teaching strategies,teaching focal points and instruction of writing skills and further to increase the writing ability of the learners.

在语际因素方面，母语的负迁移问题是讨论的主要内容；而在语内因素方面，主要就过度概括，忽视规则错误，规则的不完全应用和错误概念假设进行了讨论，并据此提出了相应的改正措施，希望对以后的写作教学策略、教学侧重点及写作技巧指导等方面有所帮助，进而帮助学习者提高写作能力。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。