查询词典 restriction

This text is an explanation and reading of wang xiaobos novel "hongfu elopes in night".the focal point lies in finding the existence circumstances question of the person that reflects in the novel, the concerned text at first is the external restriction on people, and think a kind of history that this kind of restriction is true , all show in different forms in different periods.

本文是对王小波小说《红拂夜奔》的1次解读。重点在于发掘文本中所反映的人的生存境遇问题。本文首先关注的是外界对人的限制，并且认为这种限制是1种历史的真实，在不同的时期都以不同的形式展现出来。

The restriction function of complex machine non-tree system isgiven, the restriction of non-tree system is raveled, and the dynamic modeling of non-tree system

摘 要：根据复杂机电动力学系统的特点，提出了一种约束函数递推组集法的复杂机电系统全局建模方法。

By the analysis of the characteristic and restriction of the gear components ,this paper upbuilds the characteristic model based on the restriction and actualizes data transmission and information sharing among CAD.CAE and CAPP .This paper analyzes the general principle of parameter determination and strength check about gear and researches the algorithmic of gear strength check based on the characteristic .This paper introduces expert knowledge into respective procedure of CAD.CAE and CAPP and actualizes the intellectualization in design process .strength analysis and process planning .

以齿轮类零件作为研究对象，在分析了其特征及约束的基础上，利用数据库技术和图形单元建模技术，建立了基于约束的特征模型，实现了CAD／CAE／CAPP之间的数据传输和信息共享；分析了齿轮参数确定、强度校核的一般原则，进行基于特征的齿轮强度校核的算法研究；将专家知识融入在CAD、CAE、CAPP各个过程中实现了设计过程、强度分析和工艺分析的智能化。

In the research, the study of car impact test and numerical simulation method are both used. Based on works that have done, an impact model containing car model, dummy and restriction system model was built. According to national regulation, different types of impacts were simulated. The fuel cell auto's impact safety was analyzed, and the injury criterion for occupant can be read out directly by simulation data. The main research content of this project is to set up the fuel cell auto's frontal impact model, side-impact model, rear-impact model and the whole frontal impact model, which contains car model, dummy, restriction system model and so on, and calculating these models.

在本课题的研究过程中，博采众长，坚持创新，在综合分析总结前人研究经验的基础上，将实车碰撞试验研究与仿真计算分析方法结合起来，统一车身耐撞性有限元计算分析与乘员在约束系统作用下的运动响应的多体动力学分析，采用整车虚拟碰撞试验法进行燃料电池轿车的碰撞安全性研究，即建立一个集车辆、人体模型及乘员约束系统三者于一体的完整的车辆模型，以国家安全法规为依据，进行各种碰撞型式的整车虚拟碰撞试验，利用仿真计算结果进行燃料电池轿车的碰撞安全性分析，直接预测乘员的伤情指标。

Stomatal restriction was the main factor for the P's decrease in the leaves of both alfalfas under light drought stress, while the non-stomatal restriction was the main factor for the P's decrease in the leaves of these alfalfas under medium and severe drought stresses.

2轻度干旱胁迫下气孔限制是两种紫花苜蓿P降低的主要因素，中度和重度干旱胁迫下非气孔限制是P降低的主要因素。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

Methods To choose 24 adult healthy male SD rats, take the first molar and middle slice tooth of right upper jaw as experimental teeth, divide them into 4 groups: Normal force 3-day group; force with restriction of sleeping 3 -day group; Normal force 7-day group; force with restriction of sleeping 7-day group.

取成年健康雄性SD大鼠24只，采用大鼠右侧上颌第一磨牙和右侧上颌中切牙为实验牙，随机分为4组：正常加力3天组，睡眠限制加力3天组，正常加力7天组，睡眠限制加力7天组。

Due to the restriction from social environment and psychology of public language and the inter-restriction between pursuing dialect brink and seeking dialect protection, Hankou dialect variation is on the state of relatively steady sitzkrieg. Without tremendous force from society, it is difficult to expand social distribution of Hankou dialect words variation.

由于社会环境及公众语言心理等因素制约，追求方言边缘化和寻求方言保护的两种语言心态相互牵制，使汉口话变异处在相对稳定的胶着状态之中，如果没有来自社会的强大力量推动，扩展汉口话词语变异的社会分布便不容易。

Research has been done on the coupling form between the two-row planetary gear train and the shift restriction based on the matrix, and then the method of establishing mathematics model has been presented to analyze the planetary gear transmission using MATLAB. The coupling-matrix, restriction-matrix and transmission matrix form the model. All coupling-matrices corresponding two kinds of the coupling form determine the planetary gear transmission model.

基于矩阵理论对双排行星齿轮变速机构的行星排之间联结、换挡约束进行研究，提出用联结矩阵、约束矩阵和传动矩阵构建数学模型并使用MATLAB进行传动分析的方法。2种方式的联结形式所对应的联结矩阵可以确定机构所有可能的结构型式，据此得到双排行星齿轮变速机构全部型式谱图。

The circulation, consumption and reproduction of the trilogy have not only testified to its historical significance and literariness, but also invested it with new meanings.

该三部曲的流通、消费与再生产不仅验证了其历史意义及文学性，而切还赋予它以新的意义。

If he thought that he could buy me off, he would be wrong.

如果他认为他可以收买我，那他就大错特错了。