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Haplotype II and III are shown the type of yellow cattle, and Haplotype I is shown in that of zebu cattle. In Japanese black cattle, only one of these restriction endonucleases, HindIII, was found to be polymorphisms.

日本和牛相应的11种内切酶分析中只有1种酶的酶切类型存在多态，共出现34个酶切位点，13种限制性态型，归结为2种线粒体DNA单倍型。

Today, it is a violation of an agricultural zoning restriction.

今天，这是一个违背了农业大纲限制。

A human transforming growth 〓 cDNA was inserte in reverse orientation into the retroviral vector, pLXSN. Restriction en zyme analysis was used to confirm the antisense orientation of 〓〓 in the pLXSN vector from individual transfomants.

将人的转化生长因子-〓cDNA（1467bp）反向插入逆转录病毒载体pLXSN，筛选转化菌，挑取抗性克隆，用限制性内切酶酶切鉴定重组子。

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

Currently,in the organization and guidance of urbanization,local governments have noticeable advantages in political and economic resources, but also confront with the restriction of some unfavorable factors in the structure of political,economic,cultural,social and administerial systems.

当前我国地方政府在组织领导地方城镇化的进程中，既有可资凭借的显著的政治资源优势，经济体制优势，同时也面临着政治、经济、文化、社会与行政体制结构中的不利因素的制约。

The alternant sex of webpage of 5 static state is poorer, there is bigger restriction in functional respect.

5静态网页的交互性较差，在功能方面有较大的限制。

Analogically it may be considered that the restriction of the immediate witnesses to these 3 disciples underline that the Transfiguration is a culminating event of Jesus' revelation and the mystery of his death and Resurrection.

类推可能是考虑到当下立即性见证的限制，而藉著三个门徒来衬托出改变容貌是将耶稣的启示以及死亡、复活的奥迹推至极点。

Methods With polymerase chain reaction-single-strand conformation polymorphism combining argentation and glue retrieval, DNA sequencing, and restriction fragment length polymorphism, the SNP of the 50 exons in all the coding regions of ABCA1 gene was detected in 112 patients with established diagnosis of coronary heart disease.

通过聚合酶链反应－单链构象多态性分析结合银染后胶回收、DNA测序和限制性内切酶酶切分析方法检测112例明确冠心病诊断患者的ABCA1基因全部编码区50个外显子的SNP。

The main factors, which affect the development of the performance audit of Chinese Government, include astriction of traditional ideas, system influence, restriction from unsuitable statutes, and lag in theory, etc..

传统观念的束缚、体制的影响、法规的制约、理论研究的滞后等是制约我国政府绩效审计发展的主要因素。

For general nonautonomous case, by means of the lower and upper averages of a function, under the conditions that the restriction on coefficients of the systems is relaxed further, some new results on the logistic equation are obtained, and sufficient conditions are given for permanence and global attractivity of the systems.

对于一般非自治情形，借助于函数的上、下平均值，在进一步放宽了对系数限制的情况下，我们得到logistic方程的一些新结果，给出了系统持久和全局吸引的充分条件。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。