查询词典 restriction

The swimming club is open to families in the neighborhood without restriction.

這個游泳俱樂部對鄰近地區的家庭開放，不受限制。

Methods The total RNA was extracted from the brain of human embryo. The segment of open read frame of DOC-1 was amplified by RT-PCR and was further cloned into the vector, pMD18-T, and the prokaryotic expression vector, pGEX-4T-1, by turns, to produce the new construct pGEX-4T-1-DOC-1. After restriction enzymes digestion analysis and sequenced, pGEX-4T-1-DOC-1 was transformed into E.coli BL21（DE3）. GST-p12 recombinant protein was expressed under IPTG induction and purified by affinity column.

从人胎脑组织中提取总RNA，经逆转录-聚合酶链式反应扩增DOC-1的CDS序列，再通过基因重组技术将该基因片段依次克隆到pMD18-T和pGEX-4T-1载体中，构建融合表达载体pGEX-4T-1-DOC-1，经酶切、测序鉴定后，用该重组质粒转化E.coliBL21，用IPTG诱导表达，Glutathione Sepharose 4B柱亲和层析纯化重组蛋白。

With the restriction of water demand and supply, the objective function optimal value was calculated by sequential quadratic programming.

以需水量和可供水量为约束条件，采用序列二次规划法求解函数最优值。

Methods Polymerase chain reaction and restriction fragment length polymorphism was used to identify mdm2 genotypes. The Pearson Chi square test and Woolf statistic method were used to analyze the relative risk and 95% confidence interval in order to find the genetic association between polymorphism of mdm2 gene and symptoms and pathological types of NSCLC.

采用聚合酶链反应和限制性内切酶片段长度多态性方法检测88例NSCLC患者的mdm2基因型，并通过Pearson卡方检验及Woolf方法计算相对风险度及95%可信区间（95%CI），验证mdm2基因多态性与各临床症状及病理类型之间的关联性。

This thesis focuses on the orthogonality restriction in traditional modulation methods.

当前通信事业得到了飞速发展，但是调制方法的研究进展却相形见绌。

This paper first looks back to the historical process of the development of China's hotel group,then points out that there exists the individual character of ignoring hotel group:to overlook the restriction and influence of market environment to hotel group in its development and overstress the importance of capital links. Finally a theoretical framework is presented for its future development.

本文首先简单回顾了我国饭店集团发展的历史进程，然后指出了在这一发展过程中，出现了忽视饭店集团个体特征、忽略了市场环境对饭店集团成长过程的制约和影响及过分强调资产纽带的重要性等三大误区，最后作者针对中国饭店集团化今后的发展、研究提出了一个概括性的理论框架。

After linearisation by restriction enzyme PacI, the recombinant plasmids were transfected into HEK 293-cells and amplified to give large quantities of infectious recombinant adenovirus carrying the sequences of si-FOXO1s, designated Ad-si-FOXO1-1, Ad-si-FOXO1-2, Ad-si-FOXO1-3, Ad-si-FOXO-pan and Ad-NC, respectively.

人工合成si-FOXO1s的cDNA序列，退火形成的双链cDNA克隆到腺病毒表达载体pAdTrack-CMV上，经PmeI线性化后与pAdEasy-1细胞内同源重组，最后在HEK293细胞中包装为重组腺病毒，分别命名为Ad-si-FOXO1-1、Ad-si-FOXO1-2、Ad-si-FOXO1-3、Ad-si-FOXO-pan和Ad-NC。

The expression of CD59 on the cell membrane was tested by cell immunohistochemistry and flow cytometry. Results pALTER plasmid containing CD59 was cut with restriction enzymes and a 496 bp fragment obtained by electrophoresis, which was complete conformity with the insert.

将两种含有不同突变体的人CD59全长cDNA序列重组pALTER质粒，应用阳离子脂质体导入法与pcDNA共转染CHO细胞，用G418筛选阳性克隆，应用细胞免疫组化及流式细胞仪检测CD59在CHO细胞表面的表达。

Shoe needle still makes toenail grows too closely accept restriction, happen embed armour, be like intercurrent infection, often also concern with the extruding of shoe for paronychia; callus, clavus namely.

鞋尖过紧还使趾甲生长受限制，发生嵌甲，如并发感染，即为甲沟炎；胼胝、鸡眼也往往与鞋子的挤压有关。

Subclones used for synthesis of antisense RNA probes were generated and identified as PKCa and PKCP by partial sequence analysis, restriction mapping,and comparison with the published mouse PKCa (32) and PKC,8I (33) sequences.

亚克隆用于合成的反义核酸探针产生的，并确定为pkca和pkcp部分序列分析，限制性测绘，并与已公布的小鼠pkca （ 32 ）和pkc ， 8i数据库（ 33 ）的序列。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。