查询词典 restriction crack

Objective To evaluate the ability of restriction endonuclease analysis by pulsed field gel electrophoresis and random amplified polymorphic DNA analysis for molecular typing of Neiss eria gon orrhoeae isolates.

目的 评价脉冲场凝胶电泳限制性内切酶分析及随机扩增多态性DNA分析对淋球菌分离株进行基因分型的能力。

To evaluate the ability of restriction endonuclease analysis by pulsed field gel electrophoresis and random amplified polymorphic DNA analysis for molecular typing of Neiss eria gon orrhoeae isolates.

评价脉冲场凝胶电泳限制性内切酶分析及随机扩增多态性DNA分析对淋球菌分离株进行基因分型的能力。

The reaction system of AFLP (Amplified Fragment Length Polymorphism) in genus Eriobotrya was studied and the AFLP analysis system was established by detected the results of several reactions such as DNA extraction, enzymes restriction, adapter-ligation, pre-amplification and selective amplification; the main factors which affected AFLP analysis were analyzed.

通过检测AFLP分析中DNA提取、酶切及连接、预扩增和选择性扩增的结果，对影响AFLP分析的主要因子进行分析，建立了AFLP分析体系。

But because of the restriction of our country 's system, this kind of evolutive trend would be slow.

但由于我国制度约束，这种演化趋势将是缓慢的。

After the exposal of Enron, World-Cominc and Xerox's false account scandal, there is a cry for the innovation of corporation account system all over the world. All the people are thinking of the existing corporation management system and seeking a better encouraging, restriction, supervision and balance system for all the management of listed companies.

随安然、世通、施乐公司会计假帐丑闻的爆发，全球性的公司会计制度改革呼声日渐高涨，人们纷纷反思现有的公司法人治理结构，寻求更完善的对上市公司管理层的激励、约束、监督和制衡机制。2002年7月15日美国参议院一致通过的公司改革法案，就是这种努力的集中反映。

For example, blood urea concentration as an accurate measure of kidney function and the concept of extrarenal azotemia were first reported at that time.19 It was then believed that the kidney expended considerable energy to excrete urea into the urine and that moderate restriction of protein was beneficial to human patients as contended by Addis.20 This concept fell out of favor in human medicine when it was realized that renal work is tied closely to active sodium reabsorption and that urea is passively handled.

举例来说，血中尿素浓度依照肾脏功能的准确测量和肾外尿毒症的概念都是在当时第一次揭露。19而那时大家相信肾脏花费相当多能量以分泌尿素到尿液里，然而Addis主张适当限制蛋白质的摄取，是有益於患者。20这个观点赞同人类服用的药物上使用，当肾功能被积极钠紧密连接重新吸收，而尿素却是处於被动控制。

The API of fabaceous valve has restriction I remember Somebody has written a Yi Dou....

豆瓣的API有限制我记得有人写过一个伊豆的。。。。

Fourthly, the thesis presents some variational inequality models when the objective of management optimization is max fand there is no restriction.

第四，论文给出了管理优化目标为max f时的Box约束管理优化模型。

The government's leading investment has higher cost of entering market, which brings on too bigger scale and more overlapping of investment item; the government's leading investment also has higher obstacle of withdrawing market, which induces the flexibleness of budget restriction and the aggravation of district protecting.

政府主导型投资的进入成本更低，导致此类投资的项目规模更大，重复建设更加普遍；退出障碍更高，导致与之相关的预算软约束、地方保护问题严重。

Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003～2007年临床分离的220株Pa，对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况，同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration，MIC)，筛选出对亚胺培南耐药的铜绿假单胞菌，并分析其对其它抗生素的药物敏感率；采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株，采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因，采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因，用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况；同时对产AmpC酶的Pa(25株，含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

Apllied the data of QuickBird-2——the highest resolution remote sensing,and based on the theory and methodology of fractal geometry,we have quantitatively studied remote sensing lineations in Gaolong gold deposit of Guangxi.

采用Quick Brid-2高分辨率卫星遥感数据，通过分形几何学的盒计维数法，定量研究广西高龙金矿区遥感线性构造。

So, sometimes, to change the color can not be your food (referring to the above-mentioned crustacean) has been a sign of boiling over.

所以，有时，来改变颜色，不能你的食物一直是沸腾了的迹象。