查询词典 responsive

Alco recently became even more responsive with the addition of state-of-the-art technical applications facilities.

在田纳西州Chattanooga总部和上海的技术中心，已经建立起了造纸涂料实验室，包括实验室涂布机。

Alco recently became even more responsive with the addition of state-of-the-art technical applications facilities.

近年来，Alco越来越关注于工艺过程中添加剂的应用。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

The results showed that the inhibition of the ERK pathway in dHP resulted in anhedonia and anxiety-like behavior, and the ERK pathway inhibition in the mPFC induced anhedonia and locomotor impairment in rats. The phosphorylation of the cyclic AMP-responsive-element-binding protein was decreased following the ERK pathway inhibition either in dHP or mPFC.

但值得提出的是两个脑区ERK通路在对具体的抑郁行为表现的调节上存在一定的差异，如海马ERK信号阻断引起显著的焦虑行为反应而无活力水平的降低，而前额叶皮质ERK通路阻断则表现了活力降低而无焦虑行为，从而表现了一定的脑区特异性。

METHODS AND RESULTS: Human smooth muscle cell and fibroblast cell sheets were harvested from temperature-responsive culture dishes and transplanted into ischemic hind limbs of athymic rats.

方法和结果：人类平滑肌细胞和成纤维细胞从温度敏感的培养皿中获得移植到无胸腺鼠的缺血下肢。

Chemical analysis and toxic tests were combined with benthic macro invertebrates community structure alternations. Based on tri-axial graphs together with relevant matrix of decision-making, the initial bio-responsive sediment quality criteria clearly indicated the situation of heavy metal pollution, and showed the dose-response relationships between heavy metal contamination and benthic biotics. Moreover, the SQC may provide some basic material for establishment of sediment quality standards and for follow-up remediation engineering in the area.

通过将化学分析、毒性鉴定和底栖群落结构变化三基元集成为一体的三轴图和相应的响应信息判断矩阵，基于现场生物响应的沉积物质量基准能较为清晰地指示乐安江流域表层沉积物中重金属污染的相对程度和分布模式，体现底栖生物对沉积物重金属污染的剂量-响应关系，为进一步制订该区域沉积质量管理标准、开展后续的污染治理与恢复工程提供技术保证和科学依据

Chemical analysisC and toxic testsT were combined with benthic macro invertebrates community structure alternationsB. Based on tri-axial graphs together with relevant matrix of decision-making, the initial bio-responsive sediment quality criteria clearly indicated the situation of heavy metal pollution, and showed the dose-response relationships between heavy metal contamination and benthic biotics. Moreover, the SQC may provide some basic material for establishment of sediment quality standardsSQS and for follow-up remediation engineering in the area.

通过将化学分析C、毒性鉴定T和底栖群落结构变化B三基元集成为一体的三轴图和相应的响应信息判断矩阵，基于现场生物响应的沉积物质量基准能较为清晰地指示乐安江流域表层沉积物中重金属污染的相对程度和分布模式，体现底栖生物对沉积物重金属污染的剂量-响应关系，为进一步制订该区域沉积质量管理标准 SQS、开展后续的污染治理与恢复工程提供技术保证和科学依据

In the second part of this paper, the design methodology and design method are studied, besides to review the theory of architectural design methodology, the author summarize five kinds of design method emphasized technology through lots of works, those are optimal solution, responsive method, form language, parametric planning and canonic design method.

作者从大量作品中分析和总结出五种注重技术因素的建筑设计方法：优化的设计方法、应答式设计方法、形式语言构成法、参数化设计方法、类型学与法则性设计方法。

All of these attested that there is CBF gene expression-regulated pathway and transcriptional cascade leading to the expression of cold-responsive genes under cold stress in Capsella bursa-pastoris, which ICE1 activate the expression of CBF by binding its promotor and CBF bind the DRE/CRT element in the promoter of the cold-regulated genes and activate transcription of them.

证明在荠菜中存在ICE1通过与CBF启动子部分相结合激活其表达，进而CBF又通过结合抗冻基因启动子部分带有的顺势式作用元件CRT/DRE从而促进抗冻基因表达，提高植物抗冻性的基因表达调控途径，及其它相关转录因子相互作用的级联机制。

For one thing, while the OrthoLite sockliner provides a nice cushy feel underfoot, its open celled structure is too light to be truly responsive during on-court action.

对一件事来说，OrthoLite鞋垫提供了非常好的脚感，而其开放的细胞结构，在球场上的时候提供了非常快速的响应。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失