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The results show the agglutinative activity, SOD activity, CAT activity and lysozyme activity in serum of Cyprians carpio increased in contrast to that of control group. The activity of fed lectin from Porphyra yezoensis group was 3.0, 0.8, 3.9 and 1.0 times, respedctively, as much as that of control group. The activity of fed lectin from Undaria Pinnatifida group was 1.0, 0.4, 3.0 and 0.7 times, respectively, as much as that of control group. The activity of infusing lectin from Undaria pinnatifida group was 3.0, 0.3 and 1.8 times, respectively, as much as that of control group. The activity of infusing lectin from Porphyra yezoensis group was 3.0 and 0.7 times, respectively, as much as that of control group.

实验结果表明，投喂和灌喂凝集素后，鲤血清中凝集素、超氧化物歧化酶、过氧化氢酶、溶菌酶活性与对照组相比都有提高，其中投喂条斑紫菜凝集素组比投喂对照组依次提高3.0倍、0.8倍、3.9倍、1.0倍；投喂裙带菜凝集素组比投喂对照组依次提高1.0倍、0.4倍、3.0倍、0.7倍，灌喂裙带菜凝集素组比灌喂对照组依次提高3.0倍、0.3倍、1.8倍；灌喂条斑紫菜凝集素组比灌喂对照组依次提高3.0倍、0.7倍。

The average concentration is 244 μg/ m3 and 171 μg/ m3, respectively, and the background concentration is 166 μg/ m3 and 110 μg/ m3, respectively. In the iron foundry plant, when the middle frequency furnace opened, the instantaneous PM10 and PM2.5 mass concentrations increased significantly to 10 mg/ m3 and 7 mg/ m3, respectively.

铸造厂中导致PM10与PM2.5微粒质量浓度上升的主要事件为中周波融解炉的开启，瞬间最高浓度分别可达到10 mg/ m3 与7 mg/ m3，平均浓度分别约为615 μg/ m3 与479μg/ m3，背景浓度分别约为84 μg/ m3 与61 μg/ m3。

The results showed that the general energy of the leaves, apparent digestibility and digestible energy of general energy were 15.99 MJ/kg, 68.10% and 10.54 MJ/kg respectively; crude fat content, apparent digestibility and digestible fat were 3.20%, 30.13% and 0.93% respectively; and nitrogen-free extract content, apparent digestibility and digestible nitrogen-free extract were 42.24%, 83.22% and 35.23% respectively.

结果表明：构树叶的总能、总能表观消化率和表观消化能分别15.99MJ/kg、68.10%和10.54MJ/kg；粗蛋白质含量、表观消化率和可消化粗蛋白质分别22.97%、76.5%和17.41%；粗脂肪含量、表观消化率和可消化粗脂肪分别为3.20%、30.13%和0.93%；粗纤维含量、表观消化率和可消化粗纤维分别为9.07%、32.61%和2.91%；无氮浸出物含量、表观消化率和可消化无氮浸出物分别为42.24%、83.22%和35.23%。

No differences were observed between the two groups for RDW. AUC ROCs of MCV, RDW and erythrocyte fragility in the diagnosis of thalassemia were 0.877, 0.630 and 0.796, respectively. The cut-offs of MCV, RDW and erythrocyte fragility was 88 fL, 15.9%, and 37.5%, respectively. The sensitivity and specificity of MCV were 92% and 73.5%, respectively, 73% and 58% for RDW and 85% and 75% for erythrocyte fragility.

在诊断地贫时，MCV的ROC曲线下面积为 0.877，最佳临界值为88fL，该临界值的敏感度和特异度分别是92%和73.5%；红细胞脆性的AUCROC为0.796，最佳临界值为37.5%，该临界值的敏感度和特异度分别是85%和75%；RDW的AUCROC为0.630，最佳临界值为 15.9%，该临界值的敏感度和特异度分别是73%和58%。

Results The activity, recovery rate of activity and half-life of immobilized SOD were 333 U/g, 86.32% and 43.8 d respectively. After storage at room temperature for 5 d, the relative activity of SOD was still more than 80%. The optimal reaction temperature of immobilized SOD was 45℃, and the activity retentions after being used for the first and second times were 70.12% and 51.72% respectively. Both the optimal pH values for SOD before and after immobilization were 6, while the Km values were 0.16 and 0.18 mmol/L respectively.

结果 固定化超氧化物歧化酶活力为333U/g，酶活回收率为86.32%，半衰期为43.8d；固定化酶室温保存5d后，相对酶活力仍保持在80%以上，最适反应温度为45℃，使用一次后回收率为70.12%，重复使用两次后回收率为51.72%；固定化酶与溶液酶在pH6时活性最强，Km分别为0.18mmol/L和0.16mmol/L。

Total of 161 and 166 bands were obtained respectively,and 23 random primers were used to amplify the genomic DNA of the wild and captive green peafowls.The average relative hereditary distance of the wild and captive green peafowls is 0.0555 and 0.1355 respectively;and the Shannon diversity index is 0.4348 and 1.0163 respectively.There is a prominent differentia between the two populations by T-Test of HO.

用23个随机引物，野生与笼养绿孔雀分别获得161和166个扩增片段，计算发现野生与笼养绿孔雀的种群内平均相对遗传距离分别是0.0555和0.1355，两种群间的为0.1635；两种群的Shannon多样性指数平均分别是0.4348和1.0163，有显著性差异。

The mean doses to the left and right parotid of 30% volume were (31.1±3.48) and (31.4±3.80) Gy, respectively, which those of 60% volume were (20.7±2.68) and (20.8±3.91) Gy, respectively. The patients of the mean doses ≤26 Gy and ≤30 Gy to the left and right parotids were 35.3%(12 cases) and 23.5%(8 cases), 82.4%(28 cases) and 73.5%(25 cases) respectively.

结果：左侧腮腺的平均受照射剂量为(27.5±2.67)Gy，≤26Gy者占35.3%（12例）、≤30Gy者占82.4%（28例）、30%体积的平均受照射剂量为(31.1±3.48)Gy、60%体积的为(20.7±2.68)Gy；右侧腮腺的平均受照射剂量为(28.3±2.95)Gy，≤26Gy的患者占23.5%（8例）、≤30Gy的患者占73.5%（25例）、30%体积的平均受照射剂量为(31.4±3.80)Gy、60%体积的为(20.8±3.91)Gy。

The homology of the Myostatin sequences was analyzed among species. The results showed that the mature region DNA for Myostatin of Jinhua pig has the similarity of 100%% 97%, 95%, 95%, 93%, 93%, 93%, 89%, 90%, 89%, 87%, 87%, 86%, 86%, 86%, 85% and 76% compared with that of pig, horse, human, monkey, cow, hare, goat, sheep, house mouse, rat, goose, turkey, chicken, pigeon, Xiaoshan chicken, Fan duck and zebrafish respectively. The mature region DNA for Myostatin of Xiaoshan chicken has the identity of 99%, 97%, 93%, 92%, 92%, 87%, 86%, 86%, 86%, 86%, 85%, 85%, 85%, 84%, 84%, 83% and 74% compared with that of chicken, turkey, pigeon, goose. Fan duck, monkey, human, horse, pig, Jinhua pig, house mouse, rat, sheep, hare, goat, cow and zebrafish respectively. The mature region DNA for Myostatin of Fan duck has the homology of 91%, 92%, 92%, 90%, 95%, 84%, 84%, 85%, 85%, 85%, 83%, 81%, 81%, 83%, 81%, 82% and 73% compared with that of chicken, Xiaoshan chicken, turkey, pigeon, goose, monkey, human, horse, pig, Jinhua pig, house mouse, rat, sheep, hare, goat, cow and zebrafish respectively.

将测序结果与GenBank上其它物种的相应序列进行同源性分析，结果显示，金华猪Myostatin与哺乳动物猪、马、人、猴、牛、兔、山羊、绵羊、小家鼠和大鼠的同源性分别为100％、97％、95％、95％、93％、93％、93％、89％、90％和89％，与鸟类鹅、火鸡、鸡、鸽子、萧山鸡和番鸭的同源性分别为87％、87％、86％、86％、86％和85％，与鱼的同源性为76％；萧山鸡Myostatin与鸟类鸡、火鸡、鸽子、鹅和番鸭的同源性分别为99％、97％、93％、92％和92％，与哺乳动物猴、人、马、猪、金华猪、小家鼠、大鼠、绵羊、兔、山羊和牛的同源性分别为87％、86％、86％、86％、86％、85％、85％、85％、84％、84％和83％，与鱼的同源性为74％；番鸭Myostatin与鸟类鸡、火鸡、鸽子、鹅和萧山鸡的同源性分别为91％、92％、90％、95％和92％，与哺乳动物猴、人、马、猪、金华猪、小家鼠、大鼠、绵羊、兔、山羊和牛的同源性分别为84％、84％、85％、85％、85％、83％、81％、81％、83％、81％和82％，与鱼的同源性为73％。

The total proteins from TGIF transfectant and vector control cells were separated by 2D electrophorosis. 760±41 and 680±38 spots were detected in TGIF transfectant and vector control cells respectively. Locus repeat analysis of protein spots showed that the mean deviation in IEF direction in TGIF transfectant and vector control cells were 0.864±0.123 mm and 0.843±0.115 mm respectively whereas the mean deviation in SDS-PAGE direction in these two cells were 0.812±0.109 mm and 1. 125±0.123 mm respectively. So the well-resolved and reproducible 2DE patterns from TGIF transfectant and vector control cells were established.

通过双向凝胶电泳分别分离TGIF转染细胞和PcDNA3.1空白质粒转染细胞的总蛋白质，测得两组细胞的蛋白质斑点数分别为760±41和680±38个；位置重复性分析表明TGIF转染细胞在等电聚焦方向上的平均偏差为0.864±0.123mm，在垂直板SDS-PAGE电泳方向上的平均位置偏差为0.812±0.109mm；PcDNA3.1空白质粒转染细胞在IEF方向上的平均偏差为0.843±0.115mm，在垂直板SDS-PAGE电泳方向上的平均位置偏差为1.125±0.123mm。

The hFCECs were cultured by sticking tissues piece method that the cornea were divided into the limbus and central tissue piece and digestive method,respectively.For digestive method,the digestive juice of 5mg/ml DispaseⅡ+0.25%trypsin was chosed.D/F12 with 10%fetal bovine serum and 100 U/ml penicillin and streptomycin were used in this study,the culture condition was 37℃and 5%CO2,and culture medium changed every three days2、Passage of hFCECsAfter more than 80%confluenced,cells from corneal limbus hFCECs were digested for 5-15min with different concentrations of trypsin/EDTA at 37℃,and then passaged at a ratio of 1:2.The cells were subcultured on empty plates,mouse 3T3 fibroblast feeder layer,fetal corneal stromal cell feeder l ayer or HTK feeder layer respectively.And D/F12, mouse 3T3 fibroblast conditioned medium,fetal corneal stromal cells conditioned medium or HTK conditioned medium with 10%FBS and 100 U/ml penicillin and streptomycin were used respectively as the culture medium.And the culture medium were changed every three days as well.

方法一、人胎儿角膜上皮原代和传代培养1、原代培养严格无菌操作获取人胎儿角膜片，采用组织块贴壁法、酶消化法原代培养人胎儿角膜上皮细胞。2、传代培养角膜缘部的细胞生长融合达80%以上，不同稀释浓度的胰酶/EDTA消化液消化传代分别接种于空板、小鼠3T3成纤维细胞饲养层、胎儿角膜基质细胞饲养层及HTK饲养层，培养液分别采用D/F12、小鼠3T3成纤维细胞条件培养液、胎儿角膜基质细胞条件培养液、HTK条件培养液。

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Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48