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The minimal bactericidal concentration of PTKE on Escherichia coli, Staphylococcus aureus, Salmonella, Pasteurella multocida and Streptococcus agalactiae were 100, 25, 50, 25 and 50 mg/mL respectively. The isolation of EA, EB and EC had no antibacterial effect on Escherichia coli and Pasteurella multocida. MBC of EA on Staphylococcus aureus and Salmonella were 2.5 and 5 mg/mL, and MBC of EB on Staphylococcus aureus, Salmonella and Streptococcus agalactiae were 1.25, 5 and 2.5 mg/mL respectively, while MBC of EC on Staphylococcus aureus was 2.5 mg/mL. Polygonum taibaishanense Kung.

PTKE对大肠埃希菌、金黄色葡萄球菌、沙门氏菌、多杀性巴氏杆菌和无乳链球菌的最低杀菌浓度分别为100,25,50,25和50 mg/mL;而PTKE的分离物EA、EB和EC对大肠埃希菌和巴氏杆菌无抑菌作用,EA对金黄色葡萄球菌、沙门氏菌的MBC分别为2.5和5.0 mg/mL,EB对金黄色葡萄球菌、沙门氏菌和无乳链球菌的MBC分别为1.25,5.0和2.5 mg/mL,EC对金黄色葡萄球菌的MBC为2.5 mg/mL。

Result The results showed that the substrate carbon conversion efficiency of Amygdalus mongolica Maxim. and Amygdalus pedunculata Pall. was all a constant, was 0.55, 0.57 respectively. The adapted growth temperature scope of Amygdalus mongolica Maxim. and Amygdalus pedunculata Pall. was 14-24, 13-33 ℃ respectively. The optimal growth temperature of Amygdalus mongolica Maxim. and Amygdalus pedunculata Pall.

结果]结果表明,在所测定的范围内,随温度变化蒙古扁桃和柄扁桃的底物碳转化效率为常数,分别为0.55、0.57;适宜蒙古扁桃生长的温度范围为14~34℃,最适生长温度为25℃,适宜柄扁桃生长的温度范围为13~33℃,最适生长温度为24℃。

The optimum conditions were obtained as follows: the weight ratio of fatty acids and 85%acetic acid is 1:4, ozonolysis temperature is 20-25℃, manganese/copper acetate as catalyst, and the amount of catalyst is 0.15% of fatty acids, oxidation decomposition temperature and oxidation decomposition time are 90-95℃ and 4 hours respectively. the yields of azelaic acid and pelargonic acid are 57.4% and 51.6% respectively.

以菜油酸为原料、85%醋酸水溶液为溶剂,经臭氧化、氧化分解、重结晶等过程制备壬二酸和十三烷二酸的最佳反应条件为:m:m(85%醋酸)=1:4,臭氧化温度20-25℃;氧化分解温度80-85℃,催化剂醋酸锰/醋酸铜用量为原料菜油酸质量的0.12%,氧化分解时间3h,重结晶溶剂壬酸的用量为5倍质量的氧化分解产物时,十三烷二酸的收率为58.3%,壬二酸的收率为57.2%。

Chitosan had been modified by polyacrylate sodium, glularaldehyde, and NaBH4, respectively, which afforded three kinds modified chitosan: polyacrylate sodium modified chitosan (CIS- PA4) chitosan bead and pentamethylene diamido crosslinked chitosan bead (CTSB- G- Hy). The adsorption properties of CTS-CTSB and CTSB-G-Hy were studied by L25(56) orthogonal experimental design. They show high adsorption activities for Pb2+ in solution. The highest removal efficiency of Pb2+ for CTS-PA4, CTSB and CTSB-G-Hy is 94%, 96% and 93%, respectively. The results showed the adsorption property of CTSB is the highest. The CTSB- G- Hy has good mechanical property, acid-resistance and alkali-resistance.

第二部分壳聚糖的改性及其对水溶液中铅离子的吸附性能研究以壳聚糖为原料制备了三种改性吸附剂——聚丙烯酸钠改性壳聚糖(CTS——PA_4)、珠状壳聚糖凝胶树脂和戊二氨交联珠状壳聚糖凝胶树脂,着重考察了CTSB—G—Hy的制备条件,并考察了三种吸附剂吸附6.3mg/L铅离子水溶液中铅的最佳使用条件,结果表明对CTS—PA_4而言当pb~(2+)溶液的pH在4~6.5之间,吸附剂用量为5g/L,吸附时间为1h时pb~(2+)去除率高于94%,CTS—PA_4可在酸性条件下使用,在碱性条件下依然有效但处理效果较差;CTSB对pb~(2+)具有很好的吸附能力吸附剂用量为5g/L,吸附时间为1h时处理效果极佳,pb~(2+)去除率高于96%;CTSB—G—Hy对pb~(2+)具有一定的吸附能力,吸附剂用量为3g/L,吸附时间为1.5h时处理效果最佳,Pb~(2+)去除率高于93%,而且CTSB—G—Hy的机械强度高,耐酸碱性强,可在较宽的pH范围内使用。

Results The rates of awareness about avian influenza and its infection sources were 99.0% and 83.8%,respectively;The rates of awareness about route of transmission of contact transmission,airborne infection,dungwatermouth and insect vector were 78.5%,71.2%,54.5%,70.2%,respectively;There were 45.5% people who thought all people could be infected easily by avian influenza virus;79.1% people thought fever was clinical situation of avian influenza,and that was 52.9% for nose running,cough,pharyngalgia.

结果 被调查对象对人禽流感及其传染源的知晓率分别为99.0%和83.8%;认为人群对禽流感普遍易感的为45.5%;认为禽流感临床表现有发热和流涕、咳嗽、咽痛症状的分别有79.1%和52.9%的。

Results The rates of awareness about avian influenza and its infection sources were 99.0% and 83.8%,respectively;The rates of awareness about route of transmission of contact transmission,airborne infection,dungwatermouth and insect vector were 78.5%,71.2%,54.5%,70.2%,respectively;There were 45.5% people who thought all people could be infected easily by avian influenza virus;79.1% people thought fever was clinical situation of avian influenza,and that was 52.9% for nose running,cough,pharyngalgia.

采用分层整群随机抽样方法抽取顺义区禽流感高危险职业从业人员191名,进行禽流感有关知识、信念和行为的问卷调查。

In the scorch stage, average yield of withered grass of second and third year growth prematurity type of Phleum pratense L. is 10672.5 kg/hm2, by cradling one time per year, average yield of wind hay grass is 7575 kg/hm2, the plant average height of selective colony is 160.0cm, the plant height of colony is rather equality. In the course of general nutrition composition analysis, discover that nutrition value of late-maturity type is higher than nutrition value ofprematurity type, coarse fibre and coarse protein and coarse fat of late-maturity type are 10.7%, 3.58%, 24.82% respectively, coarse fibre and coarse protein and coarse fat of prematurity type are 9.84%, 2.61%, 27.59% respectively, dry matter content of prematurity type is 92.63%, dry matter content of late-maturity type is 92.22%.

在枯黄期,第二、第三年生的早熟类猫尾草一年收割一次,枯草平均产量为10672.5 kg/hm~2,风干草平均产量为7575 kg/hm~2,选育出的群体平均高度为160.0cm,群体内植株株高较均一;在常规营养成分的分析中发现晚熟类的营养价值稍高于早熟类,晚熟类的粗蛋白是10.7%,粗脂肪是3.58%,粗纤维是24.82%,早熟类的粗蛋白是9.84%,粗脂肪是2.61%,粗纤维是27.59%,早熟类干物质含量92.63%,晚熟类干物质含量92.22%。

We found that CTC in chicken feces were nearly seldom degraded in photophobic condition.After 15 days, the degradntion rate of CTC at three different concentrations was 5.93%, 7.36%, 6.20%,respectively. But under the natural illumination, the degradation of CTC in chicken feces was very fast.After 15 days, the degradation rate of CTC at three different concentrations was 98.62%, 89.53%,95.98%, respectively.

在避光条件下鸡粪中的金霉素都比较稳定,经过15d的降解,三个不同初始浓度的药物降解幅度分别为:5.93%、6.20%和7.36%;而在自然光照条件下,鸡粪中金霉素降解都较快,在15d的试验期内,降幅达到89.53%、95.98%和98.62%,将鸡粪中金霉素浓度随时间的变化趋势进行曲线拟合,可知这种变化趋势符合一级动力学方程Ct=C_0e~。

The nucleotide sequences of the PAP gene and PAPⅡ gene in the root of Phytolacca americana were determined by reverse transcription polymerase chain reaction and PCR. The gene comprises 942bp and 933bp respectively, encoding 313 amino acids and 310 amino acids. When PAP gene and PAPⅡ gene sequences in the root were compared with those in the spring leaves and summer ones, the highest similarity 100% and 99. 8% were observed respectively. They both had one open reading frame . Analysis of the two sequences showed, in fact, they belonged to one species.

以美洲商陆根为材料,提取其总RNA、反转录,用一对PAP基因的特异性引物和一对PAPⅡ的特异性引物,分别对反转录产物进行PCR扩增,将扩增的目的片段进行序列分析,结果扩增出来的PAP基因与已经报道的序列完全相同,扩增出来的PAPⅡ基因与已经报道的序列同源性也在99.8%,而且它们都各自编码一个开放阅读框架,可以说美洲商陆根中肯定存在PAP和PAPⅡ基因,并已经转录。

The nucleotide sequences of the PAP gene and PAPII gene in the root of Phytolacca americana were determined by reverse transcription polymerase chain reaction and PCR. The gene comprises 942bp and 933bp respectively, encoding 313 amino acids and 310 amino acids. When PAP gene and PAPII gene sequences in the root were compared with those in the spring leaves and summer ones, the highest similarity 100% and 99.8% were observed respectively. They both had one open reading frame. Analysis of the two sequences showed, in fact, they belonged to one species.

以美洲商陆根为材料,提取其总RNA、反转录,用一对PAP基因的特异性引物和一对PAPⅡ的特异性引物,分别对反转录产物进行PCR扩增,将扩增的目的片段进行序列分析,结果扩增出来的PAP基因与已经报道的序列完全相同,扩增出来的PAPⅡ基因与已经报道的序列同源性也在99.8%,而且它们都各自编码一个开放阅读框架,可以说美洲商陆根中肯定存在PAP和PAPⅡ基因,并已经转录。

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