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The P release contents in the runoff sediments of the lateritic red soil of granite, lateritic red soil of sand shale and limestone soil reached the maximum in 9,7and 12 days, respectively. The phosphorus concentration on 4, 1 and 5 days exceeded river acceptable DRP critical value, respectively; therefore, it had obvious influences to water eutrophication.

花岗岩赤红壤、砂页岩赤红壤和石灰岩土径流沉积物中磷释放量达到最高所需时间分别为9天、7天和12天,磷浓度分别在第4天、第1天和第5天超过河流可接受的可溶态活性磷的临界值,因此土壤径流沉积物中磷的释放对水体富营养化具有明显的影响。

Thermal conductance of yellow-browed bunting,chestnut-flanked white-eye,hwamei and red-billed leiothrix were 0.24,0.31,0.21 and 0.34 ml O2/,respectively,below the lower critical temperature,which were 149%,149%,215%,and 243% of the expected value based on their body mass,respectively.

在下临界温度以下,黄眉NFDA2、红胁绣眼鸟、画眉和红嘴相思鸟的最小热传导分别是0.24、0.31、0.21和0.34 ml O2/,分别是体重预期值的149%、149%、215%和243%。

In the water samples from Gudao, and No. 1 and 2 wastewater stations of the Shengli Oil Field, the ratios of amount of iron bacterial cells to total microbial cells, detected by the combination of the 4 probes and DAPI staining, were 11.0%, 12.8% and 9.0%, respectively. In the iron bacterial community, Leptothrix, Sphaerotilus and L. ferriphilum were dominant in the 3 stations, and Leptothrix and Sphaerotilus, and L. ferriphilum cells to accounted for 2.94%±0.52%~3.39%±0.52% and 2.24%±0.16%~2.63%±0.49% of the total microbial cells, respectively, while L.

结果表明,利用基因探针能快速对胜利油田外排水中的铁细菌进行检测:在孤岛采油厂、胜利采油厂1号和2号综合处理站的外排水中,利用4种探针的组合探针检测到的铁细菌细胞数量分别占样品中微生物总细胞数量的11.0%、 12.8%和9.0%,其中Leptothrix和Sphaerotilus,以及Leptospirillum ferriphilum为胜利油田外排水中的3种优势铁细菌,分别占水样中总微生物细胞数的2.94%±0.52%~3.39%±0.52%和2.24%±0.16%~2.63%±0.49%;而Leptospirillum ferrooxidans和Acidithiobacillus spp。

As results showed, for limonin and naringin, the linearity was good within the ranges of 1.00-50.00 mg/L (r=0.9992) and 20.00-160.00 mg/L (r= 0.9988) respectively, and the detection limits were 0.07 μg and 0.14 μg, then the average recoveries were 98.69% and 100.13% with relative standard deviations of 2.5% and 1.5%, respectively.

实验结果表明,柠檬苦素在1.00~50.00 mg/L时线性关系良好(r=0.9992),检出限为0.07 μg,平均加标回收率为98.69%,相对标准偏差为2.5%;柚皮苷在20.00~160.00 mg/L时线性关系良好(r=0.9988),检出限为0.14 μg,平均加标回收率为100.13%,RSD为1.5%。

In addition, SW480 and HCT116 cells were treated with deoxycholic acid for 15, 30, 45, 60, 90 and 120 minutes. It was found that the deoxycholic acid treated cells showed an elevation of ATP activity by 12.35 %, 20.08 %, 29.69 %, 33.56 %, 45.85 %, 54.32 %, respectively. While the HCT116 treated cells, showed an elevation of ATP activity by 3.69 %, 5.59 %, 8.52 %, 12.26 %, 16.52 %, 19.71%, respectively.From the experiments conducted using the cell lines described above, it was further confirmed that genes CYP7A1 (cytochrome P450, family 7, subfamily A, polypeptide 1), AKR1D1 (aldo-keto reductase family 1, member D1), ALDH1A1 (aldehyde dehydrogenase 1 family, member A1), BAAT (bile acid Coenzyme A: amino acid N-acyltransferase), ABCA1 (ATP-binding cassette, sub-family A, member 1), APOC3 (apolipoprotein C-III), LIPA, LIPE (lipase, hormone-sensitive), PTGER2 (prostaglandin E receptor 2) and PTGS2 (prostaglandin-endoperoxide synthase 2) were up-regulated.

此外,为了证实其脂肪代谢产物胆酸对细胞癌化的影响,亦使用SW480和HCT116细胞株,经由脱氧胆酸在15分钟、30分钟、45分钟、60分钟、90分钟和120分钟处理后,其细胞增生比率分别有12.35%,20.08%,29.69%,33.56%,45.85%和54.32%(p.05)以及3.69%,5.59%,8.52%,12.26%,16.52%和19.71%(p.05)上升情形,并从这些细胞株进一步证实CYP7A1(细胞色素P450-7A1)、AKR1D1(醇醛酮还原酶-1-D1)、ALDH1A1(醛脱氢酶-1-A1)、BAAT(胆酸辅酶A:氨基酸N-醯基转移酶)、ABCA1(三磷酸腺苷结合盒转运体A1)、APOC3、LIPA、LIPE(脂肪酶/激素敏感脂肪酶)、PTGER2(前列腺素E受体2)和PTGS2(前列腺素内过氧化物合成酶2)等基因表现皆有呈现向上调控的情形。

Bone marrow mesenchymal stem cells could differentiate into osteoblasts, adipocytes and neural like cells with osteoblast inductor (β-sodium glycerophosphate, dexamethasone, vitamin C), lipoblast inductor (dexamethasone, 3-isobutyl-1-methylxanthine, bovine insulin, indometacin) and serum-free medium inductor (dimethyl sulphoxide, butylated hydroxyanisole) respectively. Osteoblast marker (alkaline phosphatase, osteocalcin mRNA, calcium node), adipocyte marker (lipid droplet, PPAR γ-2mRNA) and neural cell-like marker (nissl body, neuron specific enolase, neurofilament protein) were respectively determined by the immunohistochemical method, polymerase chain reaction and immunocytochemical method.

分别采用成骨细胞诱导剂(β-甘油磷酸钠,地塞米松,维生素C)、成脂肪细胞诱导液(地塞米松,甲基异丁酸黄嘌呤,牛胰岛素,吲哚美辛)及二甲基亚砜和羟基丁酸苯甲醚无血清培养基诱导剂干预细胞向成骨、脂肪、神经细胞分化,经免疫组织化学染色、PCR、免疫细胞染色方法检测成骨标志物(碱性磷酸酶、骨钙素mRNA、钙结节)、脂肪标志物(脂滴、PPARγ-2mRNA)、以及类神经标志物(尼克氏体、神经烯醇化酶、神经丝蛋白)。

Results:Among 98 patients at presentation,the sensitivities of FDG-PET and CT were 73% and 70%,respectively (P.01) in detecting lymphopathy,and 87% and 45%,respectively (P.01) in detecting extranodal lymphoma .

治疗后81例104处病灶,FDG-PET敏感性和特异性分别为81%和68%;CT的敏感性和特异性分别为55%和33%,FDG-PET的敏感性和特异性高于CT(P.01)。

The side effects in two groups were observed.Results 3~7 days after the end of treatment,the cure rate and efficacy rate were 64.3%and 82.5%in control group and 76.2%and 91.5%in trial group,respectively.3~7 days after menorrhea,the cure rate andefficacy rate were 65.9%and 83.3%in control group and 78.5%and 92.3%in trial group,respectively.There was a significant difference between two groups.

结果 停药后3~5 d,对照组的治愈率和有效率分别为64.3%和82.5%,试验组的治愈率和有效率分别为76.2%和91.5%;停药后下次月经来潮干净后3~7 d对照组的治愈率和有效率分别为65.9%和83.3%,试验组的治愈率和有效率分别为78.5%和92.3%;两组比较有显著性差异(P.05)。

By using two-stage separations indicates that, the content of total ketones compound by one stage separation is 12.14%, far lower than 54.95% by two-stage separations, and the content of pulegone and menthone increases from 3.37% and 3.46% by one-stage separation to 19.34% and 22.95% by two-stage separations respectively. But the content of the long chain unsaturated fatty acid and its ester ingredient reduces from 41.58% and 18.88% by one stage separation method to 29.06% and 9.86% by two-stage separations method respectively, also the phenol compound by two-stage separations method can been separated completely.

对荆芥挥发油采用两级分离的研究表明:酮类化合物的总含量由二级分离的54.95%远高于一级分离的12.14%,其中胡薄荷酮和薄荷酮的含量分别由一级分离的3.37%和3.46%增加到二级分离的19.34%和22.95%,而长链不饱和脂肪酸及其酯类成分含量分别从一级分离法的41.58%和18.88%降低到二级分离法的29.06%和9.86%,且二级分离法已完全除去酚类化合物。

Results Both EDS-40 and EFS-40 solutions had vitrifying effects. The survival rates of EFS-40, EDS-40 and control group were 69.6%, 82.14% and 86.78% respectively; the merogenic rates of EFS-40, EDS-40 and control groups were 54.4%, 66.96% and 80.99% respectively.

结果 两种玻璃化溶液能达到玻璃化效果;EFS-40、EDS-40及对照组冷冻复温后的存活率分别为:82.14%、86.78%、69.6%;EFS-40、EDS-40及对照组冻胚的卵裂率分别为:66%、96%、80.99%、54.4%。

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