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Under two levels of earing fertilization (N0 and N2), compared with 25℃ treatment, heat stress (35℃) accelerated the grain filling and resulted in less grain weight, and also reduced the two glutelin subunits content of both fertilization treatments 12.1% and 5.7% respectively, and reduced the prolamin content of them 6.7% and 4.9% respectively. Simultaneously, the polypeptides above 57 kD was increased 18.8% and 11.7% respectively, and grain crude protein content was also increased 0.56% and 0.35% respectively.

在N0和N2两个穗肥水平下，35℃高温处理均提高了子粒灌浆速度，明显降低了子粒单粒重；35℃高温处理还使谷蛋白的两个亚基的含量较25℃处理分别降低12.1%和5.7%，使醇溶蛋白含量分别降低6.7%和4.9%，同时却使谷蛋白组分中57kD及以上多肽的积累分别增加18.8%和11.7%，且子粒的粗蛋白含量也分别增加0.56%和0.35%。

In terms of investment subject, investment from enterprises has become an important power in the speedy growth of fixed-asset investment. In 2003, among the fund of fixed-asset investment, the proportion of domestic loan and self-raised funds accounts for 15.1% and 45.8% respectively, a jump of 29.7% and 10.2% respectively; the proportion of state budgetary funds and bond is only 6.1% and 0.4% respectively, an increase of 2.4% and a decrease of 64.9% respectively.

从投资主体来看，企业自主投资已成为主导固定资产投资高速增长的重要力量。2003年，在全省固定资产投资资金来源中，国内贷款和自筹资金所占比重为20.3%和45.0%，增长幅度达82.0%和45.0%，国家预算内资金和债券资金所占比重仅6.5%和0.5%，速度分别增长9.1%和下降70.1%。

The results showed that prevalence of antibodies to R.sibirica,R.conorii and R.akari were 4%(2/50)、2%(1/50)and 2%(1/50) respectively in sheep; 3.17%(2/63)、0%(0/63)and 3.17%(2/63),respectively in pig;16.67%(12/72)、11.11%(8/72) and 2.78%(2/72),respectively among healthy persons; and 30%(36/120)、10.83%(13/120) and 7.50%(9/120) respectively in wild rats;there was no antibody to spotted fever group rickettsiae to be found in cattle serum.

结果：共检查牛血清18份，羊血清50份，猪血清63份，人血清72份，鼠血清120份，西伯利亚立克次体、康氏立克次体、小蛛立克次体抗体阳性率羊血清分别为4％（2／50）、2％（1／50）和2％（1／50）。

Strains YL001 and YL002 formed a monophyletic clade with strains of X. nematophilus with sequence homology outweighing 99% and the sequence homology to genus Photorhabdus outweighing 94%. The lag, logarithmic, stationary and contabescence phase of the two bacteria were 0-6, 6-18, 18-66 and 66 h respectively; pH of strains YL001 and YL002 reduced to 5.70 and 5.56 at 12 h, respectively, and then rose gradually to 7.74 and 8.07 respectively when culture finished. Glucose content reduced quickly during 0-18 h, and then kept stable. Amido nitrogen content reached the lowest at 12 h and then rose slowly. YL001 exhibited the highest inhibitory effects at 54 h and YL002 exhibited highest inhibitory effects at 42 and 66 h respectively on B. cirerea and B. subtilis. Strains YL001 and YL002 belonged to X.

YL001和YL002菌株与嗜线虫致病杆菌种内菌株形成一个类群，序列同源性大于99%，与发光杆菌属内菌株的序列同源性大于94%。2菌株的延缓期、对数生长期、稳定期和衰亡期分别为0~6，6~18，18~66和66 h；培养12 h后，YL001和YL002菌株发酵液的pH值分别降低至5.70和5.56，此后逐渐上升，至发酵结束时其pH值分别为7.74和8.07；培养0~18 h时2菌株发酵液中还原糖含量迅速降低，此后保持稳定；12 h时氨基氮含量达到最低，此后开始缓慢上升；YL001菌株培养54 h后及YL002菌株培养42和66 h后，其对番茄灰霉病菌和枯草芽孢杆菌的抑制作用最强。

Bieti lost its habitat continuously in recently decades associated with the extension of rangelands and farmlands, as well as firewood collection etc. The monkey live mainly in primary fir forest and the mixed conifer forest, to evaluate the status of the monkeys' habitat, we employed GIS and RS software to identify the habitat types with five Landsat TM/ETM+ satellite imagery in winter of 1986, 1992, 1997, 2001 and 2006 years respectively. The work resulted in: 1 the size of summer grazing lands, farmlands, and fir forest was 13 100 hm2, 6 400 hm2, and 30 500 hm2 in 2006 respectively; 2 during the past 20 years (1986-2006), the size of fir forest (including primary fir forest and the mixed conifer forest) decreased by 14.6%(5 200 hm2), summer grazing lands and farmlands increased by 47.2%(4 200 hm2) and 14.3％(800 hm2) respectively; and 3, during the past 20 years, the number of firry forest patches increased by 68.4%, the mean size of firry forest patches decreased by 49.3%(from 15.1 to7.6 hm2), the largest patch index of firry forest decreased 54.9%; the patch richness had no change, but the Shannon's diversity index and the Shannon's evenness index increased by 2.7% respectively.

为了评估该物种的栖息地现状和变化情况，我们通过野外调查工作，应用GIS和RS技术，分别解译了1986年、1992年、1997年、2001年和2006年的Landsat TM/ETM+冬季卫星影像，并对解译结果进行了计算和分析，得到了以下西藏种群栖息地的主要结果：1）现有暗针叶林（包括原始针叶林和针阔混交林）面积是30 500 hm2，夏季牧场面积是13 100 hm2，农田面积是6 400 hm2；2）在过去20年间（1986-2006年），暗针叶林面积减少了14.6％（5 200 hm2），夏季牧场面积增加了47.2％（4 200 hm2），农田面积增加了14.3％（800 hm2）；3）在过去20年间，暗针叶林的斑块数量增加了68.4％，平均斑块面积下降了49.3％（从1986年的15.1 hm2下降到2006年的7.6 hm2），最大的斑块指数下降了54.9％；景观丰富度并没有变化，但Shannon多样性指数和Shannon均匀度指数分别增加了2.7％。

In group of steel bind,tension band wiring and NT-PC,the mean time of postoperative walking were respectively 35,18,10 days,the mean time of postoperative normal gait were respectively 48,45,22 days,the mean time of genuflex to 90 degrees were respectively 50,30,26 days.The excellent and good rates of steel bind(26 cases),tension band wiring (5 cases) and NT-PC (41 cases) were respectively

所有骨折均临床愈合，以术后不同时期膝关节功能和X线检查综合判断疗效，钢丝环扎固定、克氏针张力带固定、NT-PC固定三组中，术后下地行走平均时间分别为35、18、10d，术后正常步态平均时间分别为48、45、22d，屈膝90°时间分别为50、30、26d，三组优良率分别为79·2%、50·0%、91·9%。

Furthermore, out of 497 fAFLP markers, 80 special bands were found to be able to distinguish the four groups from each other and may be applied for germplasm characterization and molecular assistant classification of Meretrix clam.4 Molecular classification of two species of Meretrix clam based on fAFLP and ITS sequences4.1 The results of fAFLP maker analysis of S, G and W showed that each group had their own specific loci among which there were 53 special loci in W group, much more than those of S group (14) and G group (21). Among the 53 loci, nine were all dominant loci. These unique loci could be taken as molecular markers to distinguish W from other groups. The genetic similarity indexes and distance matrix between S and G groups were 0.9585 and 0.0424 respectively, but the genetic similarity indexes and distance matrix between W group and S or G group was 0.7939 or 0.7941, and 0.2308 or 0.2305 respectively. The results revealed that significant difference existed between W and S or G groups in molecular genetic structure. The phylogenetic trees by the methods of UPGMA and NJ also indicated that S and G populations were very closely related, while W population was a relatively independent cluster, lying beyond the species which S and G belong to.4.2 The internal transcribed spacer region of the rDNA from S group, G group and W group were PCR amplified and sequenced. The results showed that the size of ITS ranged between 1266-1269bp in W group, while those in G and S groups were 1614bp and 1520bp respectively. The GC content ranged 62.32-62.62% in W group while it was 61.77% in G group. The genetic distances between three populations of W group were 0.001～0.003, but it was 0.110 or 0.147 respectively between W group and G group or S group. Phylogenetic trees by NJ method also showed that G group was very closely related to S group, while W group was a relatively independent cluster.

在457个总扩增位点中找出了53个W的特有位点，远多于S群体(14)和G(21)群体，而且在53个特有位点中有9个出现频率为100%的位点，这些位点可以作为区分其它2个群体的特征性标记；S– G群体特有的位点有112个，其中有4个位点出现频率为100%，可作为S– G群体区别于W群体的特征性标记。S群体和G群体间的遗传相似性系数为0.9585，遗传距离只有0.0424，在NJ和UPGMA法构建的亲缘关系的树状图上均首先聚在一起，说明二者的亲缘关系很近，应属于种内群体间的关系；而W与S和G的遗传相似性系数均较小(0.7939和0.7941)，相对遗传距离很大而且十分相近(0.2308和0.2305)，在亲缘关系树状图上单独分出一支，也表明W与S和G群体间的亲缘关系较远。4.2 ITS序列比较分析通过对白壳文蛤、山东文蛤和广西文蛤的ITS序列扩增电泳、PCR-RFLP分析和ITS序列分析发现，W的ITS序列长度在1266-1269 bp，而S和与G的ITS序列总长度分别为1520 bp和1614 bp；从ITS1和ITS2长度来看，W分别为739-741 bp和316-317 bp，S为895 bp和414 bp，G为987 bp和416 bp；而从ITS碱基组成来看，W的GC含量在62.32-62.62%之间，而G群体为61.77%。W的3个壳色不同群体间的遗传距离仅0.001、0.002和0.003，S与G群体间的遗传距离是0.010，说明W群体内变异很小，而S与G群体间已出现明显的遗传分化，但还均属于种内群体间的遗传变异；而W与G和S的遗传距离分别达到0.110、0.147，两个类群差异显著，已远超出种内群体间的遗传变异。

Density Functional Theory method was used to optimize the geometries of 209 PCDE molecules, 209 PBDE molecules and 75 PCDD molecules at the B3LYP/6-31G* level. Using computed structural parameters as theoretical descriptors, the forward stepwise multiple regression technique was adopted to obtain QSPR models of environmental partition properties for these POPs by using GQSARF 2.0 and SPSS12.0 for windows programs. The obtained QSPR models are as follows:(1) QSPR models for predicting subcooled liquid vapor pressure, n-octanol/water partition coefficients lgK_(ow and subcooled liquid water solubilities -lgS_(w,l of PCDEs, of which correlative coefficients (R~2) are 0.988, 0.958 and 0.959 and the root-mean-square-error of estimation are 0.134, 0.116 and 0.327 respectively.(2) QSPR models of lgPL and n-octanol/air partition coefficients lgK_(oa for PBDEs, which both contain three structural parameters. The values of R2 for the two models are both 0.997 while the values of RMSEE are 0.073 and 0.062 respectively.(3) QSPR models of lgKow and–lgS_ for PCDEs, which both have one variable (mean molecular polarizability,α). The values of R~2 for the two models are 0.978 and 0.866 and the values of RMSEE are 0.300 and 0.270 respectively.(4) The molecular structures of 24 substituted naphthaline compounds were optimized using Hartree-Fock and DFT methods at four different levels and the same means was used to obtain four three-parameter (EHOMO, q~+ andα) QSPR models of lgK_. The model at the HF/6-311G** level is the best one of which R2 is 0.9662 and RMSEE is 0.380.(5) QSPR study for environmental partition properties of PCDEs was also performed using position of Cl substitution method in which simple parameters of substitution position were taken as descriptors. The multiple linear regression was performed with GQSARF 2.0 and SSPS 12.0 for windows programs to obtain QSPR models of lgP_L, lgK_ and–lgS_ for PCDEs of which R~2 are 0.991, 0.983 and 0.965 and RMSEE are 0.311, 0.100 and 0.300 respectively.

采用密度泛函理论方法在B3LYP/6-31G*水平上对PCDEs、PBDEs和PCDDs的分子结构进行了全优化计算，以计算得到的量子化学参数作为理论描述符，采用GQSARF 2.0和SPSS 12.0 for windows统计程序进行正向逐步回归分析，建立了这些POPs的环境分配性质的QSPR模型：(1) PCDEs的过冷液体蒸汽压、正辛醇/水分配系数lgK_(ow和水溶解度-lgS_(w，l的QSPR模型，这3个QSPR模型的相关系数(R2)分别为0.988、0.958和0.959，估计的均方根误差分别为0.134、0.116和0.327；(2) PBDEs的lgPL和正辛醇/空气分配系数lgK_(oa的QSPR模型，这两个模型都包含三个分子结构参数，其R~2都为0.997，RSMEE分别为0.073和0.062；(3) PCDDs类化合物的lgK_和-lgS_w的QSPR模型，两个模型都只含一个变量，其R~2分别为0.978和0.866，RSMEE分别为0.300和0.270；(4)采用Hartree-Fock和DFT方法，在4种不同水平上优化计算了24个取代萘系列化合物的分子结构，采用上述同样的方法分别建立了四种水平上的三变量lgK_模型，通过比较得到，在HF/6-311G**水平计算得到的模型最好，R为0.966，RSMEE为0.380；(5)同时，采用氯原子取代位置方法对PCDEs的环境分配性质进行QSPR研究，建立了PCDEs的lgPL、lgK_和-lgS_的QSPR模型，其R~2分别为0.991、0.983和0.965，RSMEE分别为0.110、0.100和0.300。

Results The according perception of Normocytic, Microcytic and Non-classified RBC between phase contrast microscope and UF-100 urine sediment analytic instrument RBC-info are 91.4%, 94.4%, 83.3% respectively, the according perception between phase contrast microscope and RBC-P70Fsc are 94.9%, 95.7%, 94.7% respectively, and the according perception between phase contrast microscope and RBC Fsc-DW are 84.4%, 86.8%, 90.5% respectively, the specificity of UF-100 and phase contrast microscope in glomerular hematuria and non-glomerular hematuria are 84.3%, 88.1% and 83.3%, 87.9% respectively.

结果 观察相差显微镜与流式细胞分析仪RBC-info尿红细胞均一型、变异型、混合型形态符合率分别为91.4%、94.4%、83.3%，与RBC-70Fsc符合率分别是94.9%、95.7%、94.7%，与RBC-Fsc-DW符合率分别是84.4%、86.8%、90.5%，流式细胞分析仪与相差显微镜的特异性在肾小球性血尿和非肾小球性血尿分别是84.3%、88.1%和83.3%、87.9%。

Finally confirmed that,The thighbone and the osteoporosis morbidity is possibly connected protein 6, respectively are: The lactoferrin light chain, membrane association protein A3, the enolase, ATP gather the enzyme, the acetyl coenzyme A reductases, the myo calcium protein; The lumbar vertebra and the osteoporosis morbidity is possibly connected protein 5, respectively are: The actin, the keratin, the enolase, ATP gather the enzyme, the myosin; The thighbone and the strong bone valuable curative effect is possibly connected protein 9, respectively are: The enolase, ATP gather the enzyme, the myo-calcium protein, the creatine activating enzyme isozyme, the phosphoglyceric acid change flavor the enzyme, the myosin, the lactoferrin light chain, the pyruvic acid activating enzyme isozyme, the crown protein; The lumbar vertebra and the strong bone valuable curative effect are possibly connected protein 8, respectively are: The carbonic anhydrase, the actin, the αB-crystal protein, 3-phospho-glycerol aldehyde oxidase, the serum albumin, ATP gather the enzyme, the myosin, the enolase.

最后确认：股骨与骨质疏松发病可能相关的蛋白6个，分别为：乳铁蛋白轻链、膜联蛋白A3、烯醇化酶、ATP合酶、乙酰辅酶A还原酶、肌钙蛋白；腰椎与骨质疏松发病可能相关的蛋白5个，分别为：肌动蛋白、角蛋白、烯醇化酶、ATP合酶、肌球蛋白；股骨与强骨宝疗效可能相关的蛋白9个，分别为：烯醇化酶、ATP合酶、肌钙蛋白、肌酸激酶同工酶、磷酸甘油酸变味酶、肌球蛋白、乳铁蛋白轻链、丙酮酸激酶同工酶、冠蛋白；腰椎与强骨宝疗效可能相关的蛋白8个，分别为：碳酸酐酶、肌动蛋白、αB-晶体蛋白、3-磷酸甘油醛脱氢酶、血清白蛋白、ATP合酶、肌球蛋白、烯醇化酶。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐