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Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%,0 and 38% respectively in sorted GPA positive cells of 3 samples by FISH,and the results of GPA+ cells of the 3 cases by PCR were positive,negative and positive respectively,which were manifested by the gender after aborticide.
选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16~24周的孕妇血中分离出NRBCs,并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果,3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%;其PCR结果,分别为阳性、阴性、阳性,并在引产后得到性别证实。
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The third order nonlinear optical refractive index and nonlinear absorption coefficient are enhanced due to the quantum confinement effects and estimated to be 4×10 -12 m 2/W and 2×10 -5 m/W respectively in nonresonant condition,while 2×10 -11 m 2/W and -1×10 -4 m/W respectively in quasi resonant condition.
结果表明,薄膜的三阶光学非线性折射率系数和非线性吸收系数都由于量子限制效应而大大地增强,在非共振条件下,它们分别约为 4× 10 - 1 2 m2 / W和 2× 10 - 5m/ W,在准共振的条件下,它们分别约为 2× 10 - 1 1 m2 / W和- 1× 10 - 4m/ W。
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Aqueous extract of cotton has allelopathic effect on Raphanus sativus, Brassica campestris, Lactuca indica, Brassica campestris, Echinochloa crusgall, Setaria viridis and Abutilon theophrasti and its inhibition effect gradually strengthens with increasing concentration. Aqueous extracts from stems, leaves and roots of cotton inhibit or accelerate seed germination and seedling growth, and activity of enzyme of seedling root of cotton. While aqueous extracts from stems and leaves of cotton are 0.02 gmL^(-1), root length, shoot length, lateral root number and dry weight of cotton respectively increase by 10.3%, 3.1%, 22.9% and 2.4% over the control. The above mentioned indexes are lower than the control by 34.4%, 39.2%, 40.6% and 19.0% respectively as the aqueous extracts are 0.1 gmL^(-1). At the same time, root activity and SOD activity decrease by 38.2% and 36.7%, while POD activity and MDA content increase by 52.3% and 36.5%. Growth indexes of plant receptors increase at low concentrations but decrease at high concentrations of aqueous extracts. The allelopathic effect of aqueous extract of stems and leaves is stronger than that of root.
结果表明:棉花植株水浸提液对萝卜、白菜、莴苣、油菜、稗草、狗尾草、苘麻均具有化感效应,随着作用浓度增加,抑制效应逐渐加强;棉花茎叶和根系水浸提液对棉花种子萌发、幼苗生长、幼苗根系活力、根系酶活性有抑制或促进作用,当茎叶浸提液浓度为0.02 gmL^(-1)时,棉花根长、苗高、侧根数、干重分别比对照增加10.3%、3.1%、22.9%和2.4%,当浓度为0.1 gmL^(-1)时,上述指标与对照相比分别降低34.4%、39.2%、40.6%和19.0%,而根系活力、SOD活性比对照降低38.2%、36.7%,POD活性、MDA含量较对照增加52.3%和36.5%,不同浓度棉花植株水浸提液对棉花生长呈现"低促高抑"的规律,且茎叶浸提液作用效应大于根系浸提液。
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In the collembolan community, Sinella, Onychiurus, Isotomurus are the dominance species, the frequencies are 37.26%, 31.6%,and 21.23%, respectively.2.In the agricultural ecology of Fuyang, the dominant species are Collembola and Acari. Their frequencies are 66.37% and 16.72%, respectively. Liposcelididae, Ceratopogonidae, Chironomidae, Staphylinidae, Nematoda, Enchytraeidae are common species.
弹尾目昆虫对重金属污染的反应,不同的种之间有很大的差别,其中,裸长角属对Cu污染反应不灵敏,陷等属、棘属对Cu污染反应敏感,随着污染程度的加剧,它们的数量明显减少;小等属和长属对Cu、Zn、Cd、Pb等重金属元素的复合污染反应很敏感,而裔符属、球圆属、土属、圆属和鳞长属对重金属复合污染反应很迟钝,在这些重金属复合污染严重的农田中仍能很好地生存繁衍。
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The homology of the Myostatin sequences was analyzed among species. The results showed that the mature region DNA for Myostatin of Jinhua pig has the similarity of 100%% 97%, 95%, 95%, 93%, 93%, 93%, 89%, 90%, 89%, 87%, 87%, 86%, 86%, 86%, 85% and 76% compared with that of pig, horse, human, monkey, cow, hare, goat, sheep, house mouse, rat, goose, turkey, chicken, pigeon, Xiaoshan chicken, Fan duck and zebrafish respectively. The mature region DNA for Myostatin of Xiaoshan chicken has the identity of 99%, 97%, 93%, 92%, 92%, 87%, 86%, 86%, 86%, 86%, 85%, 85%, 85%, 84%, 84%, 83% and 74% compared with that of chicken, turkey, pigeon, goose. Fan duck, monkey, human, horse, pig, Jinhua pig, house mouse, rat, sheep, hare, goat, cow and zebrafish respectively. The mature region DNA for Myostatin of Fan duck has the homology of 91%, 92%, 92%, 90%, 95%, 84%, 84%, 85%, 85%, 85%, 83%, 81%, 81%, 83%, 81%, 82% and 73% compared with that of chicken, Xiaoshan chicken, turkey, pigeon, goose, monkey, human, horse, pig, Jinhua pig, house mouse, rat, sheep, hare, goat, cow and zebrafish respectively.
将测序结果与GenBank上其它物种的相应序列进行同源性分析,结果显示,金华猪Myostatin与哺乳动物猪、马、人、猴、牛、兔、山羊、绵羊、小家鼠和大鼠的同源性分别为100%、97%、95%、95%、93%、93%、93%、89%、90%和89%,与鸟类鹅、火鸡、鸡、鸽子、萧山鸡和番鸭的同源性分别为87%、87%、86%、86%、86%和85%,与鱼的同源性为76%;萧山鸡Myostatin与鸟类鸡、火鸡、鸽子、鹅和番鸭的同源性分别为99%、97%、93%、92%和92%,与哺乳动物猴、人、马、猪、金华猪、小家鼠、大鼠、绵羊、兔、山羊和牛的同源性分别为87%、86%、86%、86%、86%、85%、85%、85%、84%、84%和83%,与鱼的同源性为74%;番鸭Myostatin与鸟类鸡、火鸡、鸽子、鹅和萧山鸡的同源性分别为91%、92%、90%、95%和92%,与哺乳动物猴、人、马、猪、金华猪、小家鼠、大鼠、绵羊、兔、山羊和牛的同源性分别为84%、84%、85%、85%、85%、83%、81%、81%、83%、81%和82%,与鱼的同源性为73%。
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The total proteins from TGIF transfectant and vector control cells were separated by 2D electrophorosis. 760±41 and 680±38 spots were detected in TGIF transfectant and vector control cells respectively. Locus repeat analysis of protein spots showed that the mean deviation in IEF direction in TGIF transfectant and vector control cells were 0.864±0.123 mm and 0.843±0.115 mm respectively whereas the mean deviation in SDS-PAGE direction in these two cells were 0.812±0.109 mm and 1. 125±0.123 mm respectively. So the well-resolved and reproducible 2DE patterns from TGIF transfectant and vector control cells were established.
通过双向凝胶电泳分别分离TGIF转染细胞和PcDNA3.1空白质粒转染细胞的总蛋白质,测得两组细胞的蛋白质斑点数分别为760±41和680±38个;位置重复性分析表明TGIF转染细胞在等电聚焦方向上的平均偏差为0.864±0.123mm,在垂直板SDS-PAGE电泳方向上的平均位置偏差为0.812±0.109mm;PcDNA3.1空白质粒转染细胞在IEF方向上的平均偏差为0.843±0.115mm,在垂直板SDS-PAGE电泳方向上的平均位置偏差为1.125±0.123mm。
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The hFCECs were cultured by sticking tissues piece method that the cornea were divided into the limbus and central tissue piece and digestive method,respectively.For digestive method,the digestive juice of 5mg/ml DispaseⅡ+0.25%trypsin was chosed.D/F12 with 10%fetal bovine serum and 100 U/ml penicillin and streptomycin were used in this study,the culture condition was 37℃and 5%CO2,and culture medium changed every three days2、Passage of hFCECsAfter more than 80%confluenced,cells from corneal limbus hFCECs were digested for 5-15min with different concentrations of trypsin/EDTA at 37℃,and then passaged at a ratio of 1:2.The cells were subcultured on empty plates,mouse 3T3 fibroblast feeder layer,fetal corneal stromal cell feeder l ayer or HTK feeder layer respectively.And D/F12, mouse 3T3 fibroblast conditioned medium,fetal corneal stromal cells conditioned medium or HTK conditioned medium with 10%FBS and 100 U/ml penicillin and streptomycin were used respectively as the culture medium.And the culture medium were changed every three days as well.
方法一、人胎儿角膜上皮原代和传代培养1、原代培养严格无菌操作获取人胎儿角膜片,采用组织块贴壁法、酶消化法原代培养人胎儿角膜上皮细胞。2、传代培养角膜缘部的细胞生长融合达80%以上,不同稀释浓度的胰酶/EDTA消化液消化传代分别接种于空板、小鼠3T3成纤维细胞饲养层、胎儿角膜基质细胞饲养层及HTK饲养层,培养液分别采用D/F12、小鼠3T3成纤维细胞条件培养液、胎儿角膜基质细胞条件培养液、HTK条件培养液。
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The results indicated that: compared with mono-cropping, the mean densities of rice stem borers, leaf roller, rice hoppers and leafhoppers in scale rice-duck farming were decreased by 54.4%, 48.8%, 73.6% and 96.1% respectively, and the rate of diseased plant of sheath blight and rice blast in IF were decreased by 78.9% and 48.5% respectively. Compared with the conventional rice-duck farming, the average densities of rice stem borer, rice leaf roller, rice hoppers and leafhoppers in IF was reduced by 18.2%, 9.5%, 7.0% and 0.82% respectively, and the rate of diseased plant of sheath blight in IF were decreased by 35.5%, unfortunately that of rice blast in IF was increased by 8.0%.
结果表明:规模稻鸭生态种养水稻二化螟、稻纵卷叶螟、稻飞虱和稻叶蝉平均虫量较水稻单一种植分别下降54.4%,48.8%,73.6%和96.1%,纹枯病和稻瘟病病株率分别降低78.9%和48.5%;较常规稻鸭生态种养水稻二化螟、稻纵卷叶螟、稻飞虱和稻叶蝉量分别下降18.2%,9.5%,7.0%和0.82%,纹枯病病株率降低35.5%,稻瘟病病株率升高8.0%。
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The estimated heritibilities were 0.065 and 0.047 for interval from first to last inseminution and calving interval, respectively;Genetic correlation were 0.45 and 0.35 between IFL,CI and milk yield, respectively;phenotypic correlation were 0.17 and 0.15, respectively; genetic correlation was 0.33,and phenotypic correl...
结果表明:环境因素对母牛主要繁殖性状影响非常显著,母牛繁殖性状为低遗传力性状,各性状间存在不同程度的相关
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When 〓 is the family of bipartite graphs,the 〓 capacityand the 〓 valency of a graph G are referred to as the biparticitydenoted by β(Gand the bivalencydenoted by 〓(Gof G respectively;when 〓 is the family ofgraphs whose components are complete bipartite graphs,the 〓 capacity and the〓 valency of a graph G are referred to as the complete biparticitydenoted byc(Gand the complete bipartite valencydenoted by 〓(Gof G respectively;andwhen 〓 is the family of star forests,the 〓 capacity and the 〓 valency of a graphG are referred to as the star arboricitydenoted by st(Gand the star valency ofG(denoted by 〓)respectively.
以v记图G的完全二部分解中顶点υ所关联的π中完全二部图的个数,记〓这里π取遍G的所有完全二部分解。我们称υ为G的完全二部次。对一般的图,当图的阶数充分大时,P。〓和L.Pyber给出了υ的一个上界,即,〓。F.R.K.Chung等人及Z.Tuza的结果说明这一上界是不可改进的。对υ的具体值,即使是对某些特殊的图类,结果也知之甚少。P.C.Fishburn和P.L.Hammer给出了一个相关的结果:当n≤16时,〓,(在υ的定义中,以完全二部覆盖替代完全二部分解,即得〓。同时他们提出了下述公开问题。问题1。〓=?
- 推荐网络例句
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Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.
Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。
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I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.
我来只是现在,'歼灭战perfuming自己与胡努特,那麼,他来到和SAT 。
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The shamrock is the symbol of Ireland and of St.
三叶草是爱尔兰和圣特里克节的标志同时它的寓意是带来幸运。3片心形叶子围绕着一根断茎,深绿色。