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At present there is no POCT instrument using TRFIA technique at home and abroad, so the time-resolved fluorometer based on immunochromatography described in this paper is developed on the basis of time-resolved fluorometers and it has the advantages of both the immunochromatography and TRFIA.

针对目前国内外尚无采用时间分辨技术的POCT（Point-of-Care Testing）分析仪的现状，本论文将时间分辨荧光免疫分析和免疫层析两种技术的优点相结合，研制了一种基于免疫层析技术的时间分辨荧光免疫分析仪。

Results showed that the time-resolved fluorometer has many advantages such as sensitive, stable, easy to operate and low cost In conclusion, the time-resolved fluorometer we designed is an advanced and suitable clinical immunoassay set.

这说明我们研制的测试仪的灵敏度、线性范围、稳定性等各项技术指标都达到或接近了国外同类型产品的水平。相对于进口的同类产品，本测试仪制作成本较低。故本测试仪是一种性能先进、经济适用的临床免疫检验系统。

Based on the study of spectrum, the components and technology requirement of time-resolved fluorometer were presented And a concrete method to actualize the system of pulsed Xenon lamp and the assemblage of filter setting was developed Sound clinical evaluation project was designed At last, clinical evaluation of the time-resolved fluorometer was conducted.

在光谱研究的基础上，讨论了时间分辨荧光免疫分析测试仪的各个组成部分及其技术要求，提出了脉冲氙灯和聚光滤光系统的具体实现方法，制定了合理的临床评价方案，并对制成的测试仪样机进行了临床应用性能实验。将我们研制的时间分辨荧光免疫分析仪与国外先进同类型仪器进行一系列对比测试实验后，经相关回归分析所得出的相关系数都在99.5％以上，显著性检验小于0.01。

But our patron, warned by this disaster, resolved to take more careof himself for the future; and having lying by him the longboat ofour English ship that he had taken, he resolved he would not go a-fishing any more without a compass and some provision; so heordered the carpenter of his ship, who also was an English slave,to build a little state-room, or cabin, in the middle of the long-boat, like that of a barge, with a place to stand behind it tosteer, and haul home the main-sheet; the room before for a hand ortwo to stand and work the sails.

但是，我已被摩尔人吓破了胆，生怕再落到他们的手里；同时风势又顺，于是也不靠岸，也不下锚，一口气竟走了五天。这时风势渐渐转为南风，我估计即使他们派船来追我。这时也该罢休了。于是我就大胆驶向海岸，在一条小河的河口下了锚。我不知道这儿是什么地方，在什么纬度，什么国家，什么民族，什么河流。四周看不到一个人，我也不希望看到任何人。我现在所需要的只是淡水。我们在傍晚驶进了小河口，决定一等天黑就游到岸上去，摸一下岸上的情况。但一到天黑，我们就听到各种野兽狂吠咆哮，怒吼呼啸，不知道那是些什么野兽，真是可怕极了！这可把那可怜的孩子吓得魂飞魄散，哀求我等天亮后再上岸。我说，"好吧，佐立，我不去就是了。不过，说不定白天会碰见人。他们对我们也许像狮子一样凶呢！"佐立笑着说，"那我们就开枪把他们打跑！"佐立在我们奴隶中能用英语交谈，虽然发音不太地道。

Significant Spearman rank correlations were found between the results obtained by Luminex assay and by chemiluminescence immunoassay or time-resolved fluorescence immunoassay. Only 1 microliter of serum and 3 hours were needed for Luminex assay. With the advantages of unique capability of multiplexing and high-throughput, good reproducibility, increased detection range and sensitivity, Luminex assay would have a great potential in clinical application.

本研究应用液相芯片技术利用双抗体夹心免疫分析原理同时检测了人血清中的癌胚抗原、甲胎蛋白和乙肝表面抗原，评价了该方法的线性范围、最低检测限、精密度、灵敏度、特异度、准确度等指标，并对检测结果与化学发光免疫分析法（chemiluminescence immunoassay，CLIA）和时间分辨荧光免疫分析法（time-resolved fluorescence immunoassay，TRFIA）进行了相关性分析。

Secondly, femtosecond time-resolved studies using fluorescence depletion spectroscopy were performed on Rhodamine 700 and Oxazine 750 in solutions to study the ultrafast dynamics, such as vibrational relaxation and the energy transfer from the solute to solvent. The orientational relaxation process of these dye molecules have been study in solution by the polariton resolved FS TR SEP FD.

我们利用此技术，对LD700和OX750两种染料分子电子激发态的振动弛豫和溶质与溶剂分子间碰撞传能等超快动力学过程进行了详细地实时研究，获得了它们的激发态分子在不同溶剂和体系温度等实验条件下的飞秒时间分辨荧光亏蚀谱：并首次采用极性分辨的飞秒荧光亏蚀光谱技术研究了溶质的取向弛豫过程。

But just days after the industry resolved, in the words of Deutsche Bank's Josef Ackermann,the industry resolved"clear our house first and not leave it to the regulators to do it for us", the Group of Seven finance ministers made it clear that they took such resolutions with a pinch of salt—and vowed to oversee the spring-cleaning themselves.

金融业的危机刚刚过去，德意志银行的约瑟夫·阿克曼就说："自己的房子自己打扫，不用管理层替我们操心。"不过七国集团（Group of Seven，G7）的财政部长们明确表示，他们不完全信任金融业的自治方案，准备亲自出马进行监督。

BbPress is a bare-bones, fast, forum system, and is a work-in progress. It supports registered users, categorization, tags , topic history, resolved/un-resolved status, and a search engine.

bbPress 是个纯粹，快速的论坛系统，而且正处于发展之中。bbPress支持已注册的用户，分类，标签，话题历史，已解决的/未解决的状态，和搜索引擎。

Serial types of important multiple-pluse NMRexperiments were analyzed: J-modulated spin-echo; polarization transfer; two-dimensional NMR spectra (NOESY,INADEQUATE,homonuclear COSY,heteronuclear COSY,SECSY,homonuclear J-resolved spectra,heteronuclear J-resolved spectra).

二维谱实验，分析了NOESY实验INADEQUATE实验、同核COSY实验、异核COSY实验、自旋回波相关谱SECSY实验、同核J分解谱实验和异核J分解谱实验(涉及质子反转门控去偶、FOCSY门控去偶和RE-FOCSY门控去偶四种实验)。

Two-photon absorption; Nonlinear optics properties; Ultrafast dynamics; Time-resolved fluorescence; Time-resolved fluorescence anisotropy; Solvent effect; multibranched structure

论文导师钱士雄，论文学位博士，论文专业光学论文单位复旦大学，点击次数 19，论文页数 96页File Size4789K

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。