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In this study, the author used GISH technology to analyze the material that was resistant to take-all in test, and got three chromosome arm addition lines that carried the resistance genes derived from the amphiploid of Triticum-Haynaldia. The above arm addition lines and their resistant and sensitive parents were analyzed by RAPD method and two specific molecular markers that were tightly linked to resistant gene were obtained.

本研究先用基因组原位杂交对经鉴定抗全蚀病的材料进行分析，检测出三个抗性源于簇毛麦的端体异附加系材料，再利用RAPD方法，对这三个材料和抗感病亲本进行分析，得到了两个与全蚀病抗性基因紧密相关的特异性RAPD标记。

Results: The proportion of Gram-negative bacilli rose obviously, so did the opportunistic pathogen's and Yeasts detected rate. Most drug sensitive tests showed the drug-fast rate turned to be high. Escherichia coli, Salmonella and Citrobacter resistant and so on to fluoroquinolones was 57%~97%. These bacteria to imipenem showed excellent activity. Meticillin-resistant-staphylococcus resistant to ciprofloxacin, norfloxacin, clindamycin, tetracycline, erythromycin, cefazolin and ampicillin were 40.9%, 42.4%, 63.6%, 72.2%, 85.9%, 100.0% and 100.0%, respectively Streptococcus and Enterococcus resistant to major β-lactams-penicillin, aminoglycosides, macrolides, tetracyclines and sulfonamides were between 36.0% to 100.0%.

结果：革兰阴性杆菌比例显著升高，条件致病菌和酵母样真菌检出机会显著增多；药敏结果显示多数临床细菌对常用抗生素的耐药趋势在不断上升；大肠埃希菌、沙门菌和枸橼酸杆菌等革兰阴性杆菌对喹诺酮类药物的耐药率达57%~97%，亚胺培南则对阴性杆菌有较高的敏感覆盖率；耐甲氧西林葡萄球菌对环丙沙星、诺氟沙星、氯洁霉素、四环素、红霉素、头孢唑啉和氨苄西林的耐药率分别为40.9%、42.4%、63.6%、70.2%、85.9%、100.0%和100.0%；链球菌和肠球菌对多数β内酰胺类青霉素、氨基糖苷类、大环内酯类、四环素类以及磺胺类药物均呈高度耐药，耐药率在36%~100%。

Among the durum wheat, only few cultivars (12.2%) were resistant or moderately resistant while 58.9%of cultivars were resistant or moderately resistant among the bread wheat. Many Chinese cultivars at seedling stage were resistant to the most of Italian isolates of wheat powdery mildew.

由于意大利小麦品种或含有其亲本血缘的中国品种曾经在我国小麦育种和生产中起过比较重要的作用，因此希望这些信息对育种和植病工作者有所帮助。

Reaction of 24 Einkorn accessions to mixed inoculum of powdery mildew and stripe rust,and two T.urartu accessions UR204,UR206 and a T.monococcum accession MO205 were used for reaction to a set of 15 differential Blumeria graminis tritici isolates and 21 differential Puccinia striiformis isolates,respectively.Among them,UR206 was resistant to all 15 isolates,UR204 was resistant to 14 isolates except E11,and MO205 showed different resistant types.The results indicated that most likely UR206 and UR204 carried new powdery mildew resistance genes that were different from 18 known Pm genes and MO205 carried new stripe rust gene that were different from 24 known Yr genes.

本研究对24份一粒小麦分别进行了白粉病和条锈病混合菌种苗期接种鉴定，进一步分别用一套白粉病菌菌株(15个)对2份乌拉尔图小麦和条锈病菌小种(21个)对1份栽培一粒小麦进行接种鉴定，其中乌拉尔图小麦UR206能抵抗所有供试白粉菌菌株，UR204除对白粉菌菌株E11感病外，对其余菌株表现抗性；栽培一粒小麦MO205对不同条锈菌小种表现出不同的抗性反应，研究表明乌拉尔图小麦UR206、UR204和栽培一粒小麦MO205分别含有与已知抗白粉病和抗条锈病基因不同的新基因。

The antibiotic resistance of staphylococcus aureus was detected by K-B method. Of 33 staphylococcus aureus stains isolated from food, 28 were resistant to penicillium and ampicillin, the resistance rate was as high as 84.8%.21 stains were resistant to carbenicillin, the rate was 63.6%.5 stains were detected resistant to methicillin, which took up to 15.2%. The stains also were resistant to cephalosporin antibiotics, aminoglycoside, microcline antibiotic and tetracyclines in different levels, all strains were sensitive to vancomycin.

结果 33株食品中分离的金黄色葡萄球菌，有28株对青霉素和氨苄西林耐药，耐药率高达84.8%；21株对卡比西林耐药，耐药率达63.6%；检出了5株耐甲氧西林的菌株，耐药率为15.2%；对其他头孢类抗生素、氨基糖苷类、大环内脂类、四环素类等都出现出不同程度的耐药；所有菌株均对万古霉素敏感。

Multidrug-resistant tuberculosis is defined by bacillary resistant to at least isoniazid and rifampicin in vitro.

多重抗药性结核病(multidrug-resistant tuberculosis)是指罹患至少对最重要的两种抗结核药物isoniazid及rifampicin抗药的结核菌株之结核病。

Results totally 287 strains were isolated from the 256 positive samples , and the gram-negative bacilli were 225(78.4%,), the gram-positive coccus were 41(14.3%), snd the monilia were 21(7.3%).the distributions of clinical bacteria were respiratory tract(63.4%),urinaryract(7.0%),secretion(includingwound .3%),blood(5.9%),stool(5.2%), pucture fluid(4.9%), and other sites(7.3%). of all isolating bacterium,from the first to the fifth were ps.aeruginosa(19.5%),k.pneumoniae(16.7%), e.coli(14.3%), a.baumannii(11.8%) and psemal (10.1%).resistant rates of methecillin-resistant s.aureus,methecillin-resistant coagulase-negative staphylococci and vancomycin-resistantwere 88.2%、70.0% and 11.1% respectively;the incidence of e.coli and k.penumoniae produce extended speutrum beta-lactamase were 68.6%和65.2%, 44.6% of ps.aeruginosa isolates were resistant to imipenem; the highest examining rate of 21 kinds of monilia was candida albicans (66.7％),resistant rate of candida albicans to fluconazole and amphotricin b was 51.3％ and 1.3%.

结果 在254份检出细菌阳性标本中共培养出287株细菌，其中革兰阴性杆菌225株（78.4％），革兰阳性球菌41株（14.3%），念珠菌21株（7.3%），检出菌来自呼吸道标本占63.4%，其他标本各占5%左右；细菌检出占构成比前三位的依次为铜绿假单胞菌19.5%、肺炎克雷伯菌16.7%、大肠埃希菌14.3%；耐甲氧西林金黄色葡萄球菌、耐甲氧西林凝固酶阴性葡萄球菌和耐万古霉素的肠球菌的发生率分别为88.2%、70.0%和11.1%；大肠埃希菌和肺炎克雷伯菌的超广谱β-内酰胺酶的检出率分别为68.6%和65.2%，铜绿假单胞菌对亚胺培南的耐药率为40.2%；白色念珠菌对氟康唑的耐药率为81.3％，对两性霉素的耐药率为3.2%。

Results: Cultures for Mycobacterium were positive in a total of 2657 people, among them 1848 strains (69.55%) were Mycobacterium tuberculosis, and 809 strains (30.45%) were Nontuberculous Mycobacterium. Among the 1848 strains of Mycobacterium tuberculosis, 337 strains (18.24%) were drug resistant, and 75 strains (4.06%) of them were resistant to both Isoniazid and Rifampin which made them multi-drug resistant. Among the 548 strains of Nontuberculous Mycobacterium, 453 strains (82.66%) were drug resistant. There were a total of 261 rapidly growing mycobacteria, and 242 (92.72%) of them were drug resistant.

结果：分枝杆菌培养阳性共2657菌株/人，其中1848株(69.55%)为结核分枝杆菌，809株(30.45%)为非典型结核分枝杆菌感染。1848株结核分枝杆菌中，337株(18.24%)有抗药性问题，其中75株(4.06%)同时对Isoniazid和Rifampin有抗药性为多重性抗药菌。548株非典型结核分枝杆菌中453株(82.66%)有抗药性问题；快速生长菌群共261株，其中242株(92.72%)有抗药性问题。

Plant disease resistant reaction is a complex physiological process. To date, it has been found that many compounds are involved in the recognition of pathogens and following signaling transudation pathways.

中文题名水稻抗稻瘟病数量性状相关基因的鉴定与分析副题名外文题名 Identification and characteristics of quantitative trait loci associated genes resistant to rice blast disease in rice 论文作者郑先武导师朱立煌研究员学科专业遗传学研究领域\研究方向学位级别博士学位授予单位中国科学院遗传与发育生物学研究所学位授予日期2002 论文页码总数77页关键词水稻抗病基因稻瘟病馆藏号BSLW /2003 /S511 /97 植物对外界病原的抗性过程是一个复杂的生理现象。

The genetic study suggested that the carbendazim-resistance in these mutants could be steadily inherited by asexual and selfed reproduction, and the resistance was controlled by the same single major gene both in laboratory mutants and field resistant isolates, the different levels of resistance or the same resistant level in different strains maybe conferred by mutations at different sites or one site with different allelic mutations. The gene compensates for the sensitivity to MDPC was allelic to that governs the carbendazim resistance, but the mutation for increasing sensitivity to MDPC in this gene could get the pathogen highly resistant to carbendazim.

抗药性遗传研究表明，在所研究的抗药突变体中，抗药性在自交和无性繁殖后代中能稳定遗传；室內抗药突变体和田间抗药菌株对多菌灵的抗药性由同一个主效基因控制，但它们发生突变的位点不同或者同一碱基位点发生了不同的突变；对MDPC的敏感性也是由单个基因控制的，该基因与控制多菌灵抗性的基因是等位基因，当该基因发生对MDPC的敏感性增加的突变时会使病菌对多菌灵产生高水平抗性。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

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