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The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因,并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列,通过对克隆的降钙素序列的比较研究,结果显示同一科的鱼降钙素序列保守性较高,同时,根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究,结果显示在分类学上,分类地位较远的鱼,其降钙素相似性较低,分类地位越接近的鱼,其降钙素相似性越高;我们利用谷胱甘肽S-转移酶(Glutathions S-transferase,GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究,应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索,并将纯化的融合蛋白作为抗原,获得了高效价的兔抗鲑鱼降钙素免疫血清;生物活性研究表明,大肠杆菌表达的融合蛋白具有显著的降血钙作用。

This paper studies depression in the hot cathode glow discharge PCVD method of diamond films influence in the process, mainly from two aspects of research: a study of the process of discharge pressure of the impact of that pressure on the technology, the impact of state discharge , as well as pressure and discharge voltage of the relationship between the two, and experiment with different pressure, relative to the preparation of samples, and using Raman instrument and SEM analysis of the quality of the film, by comparing the different types of gas pressure on the quality of diamond films, summed up the most good gas pressure parameters of a high-quality diamond films.

本文研究气压在热阴极辉光放电PCVD方法制备金刚石膜过程中的影响,主要是从两个方面进行研究:一,研究制备过程中气压对放电的影响,得出气压对工艺,放电状态的影响,以及气压和放电电压的关系;二,采用不同气压进行实验,制备出相对应的样品,并用Raman和SEM等仪器对膜进行质量分析,通过对比不同气体压强对金刚石膜质量的影响,总结最佳气体压强参数,制备出高品质金刚石膜。

The research studies Chinese attribution of success and failure by using self-established questionnaires The main results indicate:(1)Chinese attribution of success and failure is composed of six factors: jinni and fate, opportunity and luck, retribution and lot, ability and effort, help of others, difficulty

采用自编的量表对成败归因进行了研究,结果表明:中国人的成败归因包括 6个因素,它们是:神灵命运、机遇运气、报应、能力努力、他助、难易程度

Through diachronic and synchronic studies and the systematic method, based on fieldwork and documental research, this paper makes an in-depth approach to the living place of folk story narrator梂ufeng Tujia Autonomous County in the west of Hubei province and the profound influence of its peculiar culture of Chinese fireplace and "jocosity" convention on the narration of the story teller and the significant influence of the story tellers narration on the development of regional culture.

本文通过田野调查与文献考证,运用民间文学整体研究的方法,深入探讨了故事家刘德培生活所在地——鄂西五峰土家族自治县独具特色的火塘文化、掰人习俗对故事家讲述活动的深刻影响,以及故事家的讲述活动对区域文化的发展所起的重要作用。

At first, this paper conducts in depth research on classification method, compaction propeties, granulometric composition and crushing of stone. On the basis of clarifying engineering characteristics of material of rock-filling embankment, requirements of ground treatment have been put forword in this paper. Then combined with the site test and engineering practice in SHUINAN Road and LANLIN Road, through the synthetical analysis of factors of influencing densification effect for the maximum grain size of filling material, lay-down thickness, granulometric composition, densification machinery and densification frequency etc., this paper studies key technical problems about construction technologies for spreading and leveling of filling material, choosing densification machinery, densification technology and slope construction systematically. Finally, construction technologies of filling lime-stone and sand-stone roadbed have been proposed in this paper ,which are significant for quality guarantee of highway engineering.

本文首先从石料的分类方法、压实特性、粒径组成以及破碎性等方面进行研究,在明确填石路基填料的工程特性基础上,进而提出了填石路基的地基处理要求,然后结合广西水南路和甘肃兰临路填石路基试验路段上的现场研究,通过对填石路基填料最大粒径、松铺厚度、粒径组成,压实机械以及碾压遍数等影响压实效果因素的综合分析,系统研究了填石路基填料的摊铺与整平技术、压实机械选型技术、压实工艺以及边坡施工技术等施工工艺中的关键问题,最后提出石灰岩类和砂岩类填石路基的施工工艺要求。

Based on several studies concerned in the personality analysis and evaluation of nuclear power plant operators, a procedure and method of this research are identified and discussed to draw a fairly comprehensive picture of an operator's personality and to use personality data as predictors of a nuclear power plant operator performance.

基于国内外有关心理特质定量研究,提出了一套完整的程序和方法,来获取核电厂操纵员全面的心理特质定量数据及利用这些定量数据来评价操纵员的行为。

Its main interests are: Clinical studies, which relate directly to the diagnosis and treatment of eye diseases and disorders; The development of devices and vocational programs to aid the partially sighted, blind and hearing impaired; Basic research to understand how the eye and brain work, providing a fundamental background for both the clinical and rehabilitation programs.

它的主要研究兴趣包括:有关眼疾病治疗和诊断的临床研究;发展相关的设施和职业项目去帮助部分视力受损、盲人和听力受损的患者;进行眼和脑工作机制的基础研究,为临床和康复项目提供基础支持。

This paper presents a review of studies of interference absorbing materials,which come in three basic types:normal interference absorber,Jauman absorber and FSS absorber. And we particularize the structures,applications and methods of analysis and design of each of these types. Results show that the FSS absorber provides a better absorption ability and is more practicable,and that active multi-layer FSS absorber represents a new direction of interference absorbing material research.

综合介绍了干涉型多层吸波材料的吸波原理及其国内外研究现状,并分别研究了干涉型多层吸波材料三种基本类型:一般干涉型吸波材料、Jauman吸收体和频率选择表面吸收体的一般结构、应用以及分析与设计方法,得出FSS吸收体较之其他类型具有更优的吸收性能和更高的实用价值,最后指出了干涉型吸波材料发展的新方向是有源FSS吸收体。

This paper presents a review of studies of interference absorbing materials, which come in three basic types: normal interference absorber, Jauman absorber and FSS absorber. And we particularize the structures, applications and methods of analysis and design of each of these types. Results show that the FSS absorber provides a better absorption ability and is more practicable, and that active multi-layer FSS absorber represents a new direction of interference absorbing material research.

综合介绍了干涉型多层吸波材料的吸波原理及其国内外研究现状,并分别研究了干涉型多层吸波材料三种基本类型:一般干步型吸波材料、Jauman吸收体和频率选择表面吸收体的一般结构、应用以及分析与设计方法,得出FSS吸收体较之其他类型具有更优的吸收性能和更高的实用价位,最后指出了干涉型吸波材料发展的新方向是有源FSS吸收体。

Its accurate error is lower than the permissible error regulated by the government in feasibility research phrase. Because of the penetrability of its process and normality and objectivity of its results, subjectivities of organizations or individuals can be avoided effectively and different estimators can get almost the same result by using it. Furthermore, its process has stronger persuasiveness than former ones. So it is obvious that the new method is better than former methods and other model predicting methods in similar studies.

经精确度分析对比和实例验证,这一新方法简捷高效、清晰合理、易于推广普及,不但能满足国家对建设项目可行性研究投资估算规定的允许精度要求,而且体现了估算过程的透明性、估算结果的规范性与客观性,能有效地避免投资估算时估算人员和组织的主观差异性,明显优于以往的估算方法和类似研究中用的模型估算方法。

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