查询词典 reproducible

Results The well-resolved, reproducible 2-DE patterns of PBMC in patients with HCC and healthy adults sere obtained. For HCC, the average spots of 2-DE maps were 1206±48, and the average matching rate as 90.8%. For normal adults, the average spots were 1123±37, and the average matching rate was 92.6%.

结果：得到了分辨率较高、重复性较好的HCC及正常人PBMC双向凝胶电泳图谱，平均蛋白质点数分别为1206±48及1123±37；组内的平均匹配率分别为90.8%及92.6%。

The total proteins from TGIF transfectant and vector control cells were separated by 2D electrophorosis. 760±41 and 680±38 spots were detected in TGIF transfectant and vector control cells respectively. Locus repeat analysis of protein spots showed that the mean deviation in IEF direction in TGIF transfectant and vector control cells were 0.864±0.123 mm and 0.843±0.115 mm respectively whereas the mean deviation in SDS-PAGE direction in these two cells were 0.812±0.109 mm and 1. 125±0.123 mm respectively. So the well-resolved and reproducible 2DE patterns from TGIF transfectant and vector control cells were established.

通过双向凝胶电泳分别分离TGIF转染细胞和PcDNA3.1空白质粒转染细胞的总蛋白质，测得两组细胞的蛋白质斑点数分别为760±41和680±38个；位置重复性分析表明TGIF转染细胞在等电聚焦方向上的平均偏差为0.864±0.123mm，在垂直板SDS-PAGE电泳方向上的平均位置偏差为0.812±0.109mm；PcDNA3.1空白质粒转染细胞在IEF方向上的平均偏差为0.843±0.115mm，在垂直板SDS-PAGE电泳方向上的平均位置偏差为1.125±0.123mm。

Results Well-resolved, reproducible 2-DE profiles of human colon carcinoma tissues and paired normal tumor-adjacent colon tissues were obtained. For colon carcinoma tissues, a total of (980±28) spots were detected; for paired normal tumor-adjacent colon tissues,(860±45) spots were detected. For normal tumor- adjacent colon tissues, the average deviation of spot position was (10.487±0.11) mm in IEF direction and (0.944±0.12) mm in SDS-PGE direction; for colon carcinoma tissues the average deviation of spot position was (0.654±0.11) mm in IEF direction and (1.20±0.22) mm in SDS-PGE direction. Number of differentially expressed proteins was 72.00±12.34 between colon carcinoma and paired normal tumor-adjacent colon tissues.

结果 建立了结肠癌及正常肠组织的双向凝胶电泳图谱，其中癌旁正常结肠组织和结肠癌组织电泳图谱中平均蛋白质点数分别为860±45和980±28；癌旁正常结肠组织和结肠癌组在IEF方向上的平均偏差分别为(0.487±0.11)mm和(0.654±11)mm，在SDS-PAGE方向上的平均偏差分别为(0.944±0.12)mm和(1.20±0.22)mm，结肠癌与癌旁正常结肠组织的差异表达蛋白质点数为72.00±12.34。

Results Well-resolved,reproducible 2-DE patterns of SGC7901/VCR and SGC7901 were established.Silver staining was better when protein sample amount was low,overloaded protein will interfere resolution of the maps.Gels stained with colloidal Coomassie brilliant blue had more protein spots numbers and abundance without apparent trails when increased loading protein sample.

结果 获得了背景清晰、重复性好的双向凝胶电泳图谱，两种染色凝胶相比，硝酸银染色在样品少时显示更佳，过量则影响图像质量，而胶体考马斯亮蓝染色在上样量增加时的凝胶蛋白质点数目及丰度均增加，并无明显拖尾。

The well-resolved, reproducible 2-DE patterns of Ganyang Huafeng syndrome and Xuexu Shengfeng syndrome PD were established and revealed the protein expression difference of PBMC between the two syndromes of PD patients.

结果 获得了分辨率和重复性均较好的双向电泳考染图谱，对其中的15个差异蛋白质点分别进行肽质指纹分析，经数据库查询，初步鉴定为一些与细胞骨架、抗氧化应激、蛋白降解、信号转导、细胞周期调控等有关的蛋白质。

objective to investigate staining methods for two-dimensional gel(2-de)electrophoresis in multidrug resistance of gastric cancer.methods cultured vincristine-resistant human gastric cancer cell line sgc7901/vcr and its parental cell line sgc7901.variant amount protein of those cells were separated by 2-de.gels were stained with silver nitrate or colloidal coomassie brilliant blue,and scanned by image scanner.results well-resolved,reproducible 2-de patterns of sgc7901/vcr and sgc7901 were established.silver staining was better when protein sample amount was low,overloaded protein will interfere resolution of the maps.gels stained with colloidal coomassie brilliant blue had more protein spots numbers and abundance without apparent trails when increased loading protein sample.conclusion two staining methods were influenced largely by the sum of protein samples,properly selection may be helpful for further study with proteomics in multidrug resistance of gastric cancer.

低甲氧基果胶的胶凝机理及防止预凝胶。。。他扎罗汀凝胶与克林霉素凝胶治疗痤疮。。。注射隆乳后经乳晕切口取出聚丙烯酰胺。。。目的分析利用蛋白质组学方法研究胃癌耐药相关蛋白质中双向电泳凝胶的染色显示。方法培养胃癌细胞sgc7901和长春新碱诱导的耐药胃癌细胞sgc7901/vcr，用双向凝胶电泳技术分离总蛋白，银染及胶体考马斯亮蓝染色，image scanner扫描仪扫描凝胶。结果获得了背景清晰、重复性好的双向凝胶电泳图谱，两种染色凝胶相比，硝酸银染色在样品少时显示更佳，过量则影响图像质量，而胶体考马斯亮蓝染色在上样量增加时的凝胶蛋白质点数目及丰度均增加，并无明显拖尾。结论两种显色方法受样品量影响较大，恰当选用有利于通过蛋白质组学研究胃癌耐药机制工作的进一步开展。

The research results are highly reproducible owing to the repeatable micro-model technique.(2) Quantifying the Kr-S-P curves in two-phase flow. The validity and adaptability of multi-phase curves are verified using micro-model testing bed tailored to adequate scale of pores. Results obtained from the P-S micro-model tests generally agree with the threshold values of displacement mechanism offered by researchers using geometric shapes. The hysteresis of .experimental Kr-S curves of numerous two-phase flows are depicted in terms of connectivity and humidification. The P-S and Kr-S curves of two-phase flow are well-derived in conjunction with micro-model test results and empirical van Genuchten model. For the curve expressing the relation between saturation and capillary pressure, experimental results of water-air flow system corresponds to the theoretical data.

2二相流体对微模型实验之Kr-S-P关系：包括以孔颈尺寸比例组成微模型板实验平台去证实多相流流动主要特性曲线正确性与适用性；验证水和空气二相流之P-S微模型实验，与前人以几何形状所推估出来的置换机制门槛值大致吻合；以连通性及润湿性等解释多组二相流体对Kr-S曲线实验之迟滞现象；以微模型实验结果配合van Genuchten特性曲线经验式互推估二相流体对之P-S与Kr-S 曲线，推估结果良好；以比例原则推估多组二相流体於毛细压力-饱和度曲线的结果，显示在水和空气二相流体对为参考流体对时，推估其他组流体对吻合度最佳。

The result of experiment reproducibility showed that SSR marker test was well reproducible, and the results were stable and reliable, and could be used as potato variety purity identification applied in practice.

试验重复性测试结果表明，SSR标记试验重复性好、结果稳定可靠，可以作为马铃薯品种纯度鉴定的方法在实践中应用。

Similarly, texture on a low-draft face, which might not be reproducible in a straight-pull mold, can be produced in a mold with side actions.

类似的，还有小拔模角度面上的晒纹。

When the redox electrode is immersed in a solution containing a reversible chemical reaction system, a migration of electrons is established between the electrode and the system. This electron flow can be construed as an exchange current density and is of paramount importance for accurate, fast and reproducible redox potential measurement.

当氧化还原电极浸入具有逆转化学反应系统的溶液中时，会有电子在电极与溶液间移动，这些电子的流动被转绎成交换电流密度，这是准确性高，快速和再现性佳的 ORP测量技术最重要的参数。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。