查询词典 reporter

Luciferase activity was assayed with Dual-luciferase reporter system and measured by Luminometer.

以Duabludferase reporter系统为底物并采用荧光测定仪测定荧光酶活性。

The reporter vectors which contain the luciferase gene and dioxin response elements are then transfected to different cell lines. When used together with a sensitive luminometer, the cells express different light intensity depending on their dioxin concentrations.

针对此传递路径的特色，我们建立两个含有戴奥辛识别单元和萤光基因的表达质体，分别送入不同的细胞株中，藉由萤光的定量做出一组标准曲线图，使其成为侦测环境污染物的工具。

Methods:The luciferase reporter plasmids of MDM2 P2 promoter containing different SNP309 were contructed,and cotransfected into CV-1 cells with AML1-ETO,AML1 and SP1 expression plasmid.The transactivities of luciferase were assayed by luminometer to determine the impact of AML1,AML1-ETO,and SP1 on MDM2 P2 promoter.

研究方法：构建含不同SNP309位点的MDM2 P2启动子的报告质粒，与AML1-ETO、AML1以及SP1的表达质粒单独或共转染CV-1细胞，测定荧光素酶的活性，分析AML1-ETO、AML1以及SP1对MDM2 P2启动子的影响。

Firstly, the pGL3-Control vector was reconstructed , the pGL3-Enhancer vector deletious of SV40 promoter gene was obtained . Then the IFN-β promoter gene was cloned into the pGL3-Enhancer vector and pGL-IP21, the Luciferase reporter plasmid with IFN-β promoter was OK. The availability of pGL-IP21 was verified by NDV ,the inductor of IFN-β, the Luciferase activity was assayed in cells transfected with pGL-IP21 by Luminometer.

首先将pGL3-Control载体进行了改构，除去了SV40启动子基因，获得了pGL3-Enhancer载体，将获得的IFN-β启动子基因连入载体中，构建了带有人IFN-β启动子基因的荧光素酶报告质粒IP-21，并且通过实验证明所构建的质粒在干扰素的诱导剂NDV的作用下能够表达荧光素酶活性，照度计检测荧光素酶活性增强，从而验证了所构建的重组质粒的有效性。

Methods:The expressive plasmids of AML1b and AML1-ETO were transfected into CV-1 and 293 cells.The expression level of endogenous pig7 gene was detected by Realtime-PCR.The luciferase reporter plasmids containing pig7 enhancer/corresponding mutant sequences were constructed and co-transfected into CV-1 cells with expressive plasmids of AML1b and AML1-ETO.The transactivity of pig7 enhancer was assayed by luminometer.

研究方法：AML1b和AML7-ETO表达质粒分别转染及共转染CV-1和293细胞，用Realtime-PCR方法检测上述基因对两种细胞内源性pig7基因mRNA表达水平的影响；构建含AML1b结合位点的pig7基因增强子序列以及相对应的突变位点的荧光素酶报告基因质粒；将表达载体/突变载体与AML1b或AML1-ETO基因瞬时共转染CV-1细胞，分析AML1b、AML1-ETO对报告基因的转录调节作用。

The proposal does not yet have the formal backing of the EU or Germany - France's largest trading partner - and according to the BBC's business reporter Joe Lynam, it is widely expected to face resistance from Britain and the US, home to the world's largest financial centres.

这项建议不仅已遭到欧盟和德法贸易同盟的反对，在BBC商事报道员JOE LYNAM 看来，其甚至可能面临英美两国的抵制，二者可是世界最大的金融中心所在地。

Looking back, I believe that my position was not machismo; it was an innate reaction as a reporter.

回头看看，我相信采取如此立场并非为了显示男子汉气概，而是出于记者的自然反应。

Reporter visited the enterprises found that as Hengda, Di MacLEOD a result of such compression plant enterprise widespread vacant factory .

记者走访了多家企业发现，像恒达、迪高乐这样压缩厂房而导致厂房空置的企业普遍存在。

Through several days of interviews, the reporter walked into the "magic circle", decrypt this "magic" Life of ethnic groups.

通过几天的采访，记者走进了这个"魔圈"，解密这个"魔力"族群的生活现状。

The first story, written by reporter David Singleton,appeared with allegations that while congressional candidate ChrisHackett was addressing the crowd and mentioned Obama's name a manin the audience shouted kill him.

如果梁文道是一个刚出道写评论文的新手，出这样的问题，也情有可原，但作为一位评论老手，却是太不应该，梁文道应该对他引用新闻"23次杀了他"做出说明，比如他的新闻报道来自那个机构等等。

Hanna: That's over now, isn't it?

都结束了，对吗

You must be ill. You look so pale.

你一定是病了，你的脸色苍白。