查询词典 reporter

After carding the development of the public sphere and the theory of contact behavior, this essay takes "Face-to-face" as a case and launches a practical and specific analysis from the public topic, the reporter's question , the interviewee's reply, bilateral gambling in sub-topic, dialog in the absence of audience and so on. This essay is trying to build a speech act classification system about the On the spot dialogue between reporter and the interviewee in television public sphere, and not On the spot dialogue between program and audience. Moreover, on this basis the essay discourses the structure reforming characteristics in television public sphere.

中文摘要论文在梳理公共领域与交往行为理论的发展脉络之后，以《面对面》为个案，从公共性话题、记者提问、访谈对象回答、双方在子话题上的博弈、受众的不在场对话等5个方面展开了实证的、具体的分析，试着建立电视公共领域中记者与访谈对象的在场式对话、节目与受众之间的不在场式对话的言语行为分类体系，并在此基础上论述了电视公共领域的结构转型特点。

Report from our correspondent (Yang Weixu of Pan Qi of East Asia reporter's exercitation reporter) this drink femaly much wine but really enough fierce, not only the dress her chaperon is lacerate, and still maul the policeman, more those who let a person want to be less than is she still stripped off trousers to make police station greatly, break the article of police station bad.

本报讯(东亚记者潘琪实习记者杨维絮)这个女的喝多了酒可真够凶的，不但将自己女伴的衣服撕碎，而且还将民警打伤，更让人想不到的是她还脱光了裤子大闹派出所，并将派出所的物品砸坏。

Two different Raman reporter molecules (4,4'-bipyridine and thiophenol), which served as respective labels for each type of antibodies, were selected, SERS has the advantage of a small line width of Raman bands and high sensibility. The presence of dual-analyte was established by distinguishing the corresponding labeled reporter's Raman spectrum.

采用不同标记分子苯硫酚和4，4'-联吡啶(4，4-Bipyridin)分别标记不同的免疫金溶胶，利用表面增强拉曼光谱谱峰较窄且具有较强的分辨率及高灵敏度的特点，通过对两种标记分子特征谱峰的判断识别所加入的两种抗原。

Gold colloids were labeled with Raman reporter mercapto molecules such as 4-mercaptobenzoic acid and thiophenol to form Fourier transform surface-enhanced Raman scattering reporter-labeled gold colloids.

以对巯基苯甲酸、苯硫酚为标记分子，与金纳米粒子生成具有SERS信号的标记金溶胶。

Really abhorrent, did not sympathize with a person, also pay no attention to what what mauled, return venomously slander, still scold me to say you are sure the head has a problem, can be here how otherwise talk rubbish, gas gets my pop eye to want as academic as her, did not think of to walk out of a man to come, say if you are solid however be be hit by those mop, the issue that also does not involve us, I am so angry that do not have a word to say, because rapid move goes receiving a daughter to classes are over, do not think again as academic as them, but answer,want to feel to subdue more more in the home, but did not cause the accident that weigh an injury to cannot accuse them again, the grievance that wants to hit reporter hot line to let a reporter understand its reason to let its say me in the newspaper is afraid of again fussily.

真可恶，一点都不同情人，也不问是否打伤了什么的，还血口喷人，还骂我说你肯定脑袋有问题，不然怎会在这儿瞎说，气得我瞪大眼睛想与她理论，没想到走出一个男人来，说你假如却实是被那些拖把打到了，也不关我们的事啊，我是气得没话说，由于急着去接女儿放学，不想再与他们理论，可是回到家里越想越觉得委屈，但没造成重伤事故又不能告他们，想打记者热线让记者来了解事情缘由让其登报说说我的委屈又怕小题大做了。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

The reporter investigates the company that deletes negative news technically to be called elegant song times for the person to on the net, the inquiry deletes the price of negative news, a gentleman of this company that surnames bavin expresses to the reporter, unless network major cutout often has sense of property, not be what content should give money to be able to be deleted only.

记者在网上查到一个专门替人删除负面新闻名叫雅歌时代的公司，询问删除负面新闻的价格，该公司一位姓柴的先生对记者表示，网络专业删除非常有分寸，并不是什么内容只要给钱就可以删除。

According to the classical signal transduction pathway of estrogen, we use Luciferase reporter gene to screen phytoestrogen form Selaginella tamariscina, Pinus massoniana Lamb, Corallodiscus flabellate, Dryopteris sublaeta Ching et Hsu and Leonurus heterophyllus. In this study,the pβgal-Control vector, the pERE-TAL-luc vector, the pCNX2-hERα/pCNX2-hERβvector were transfected into HEK293 cell line. If the components in the herbs combine with the ER, then it could induce the expression of reporter gene controlled by ERE.

根据体内的雌激素信号转导途径，我们选用了以luc为报告基因、ERE为调控序列的重组报告基因载体pERE-TAL-luc，并将其与重组人ERα(humanERα，hERα)表达载体pCXN2-hERα和重组人ERβ(humanERβ，hERβ)表达载体pCXN2-hERβ共转染ER阴性细胞系，建立了靶向ERα或ERβ的细胞水平药物筛选模型，结合小鼠子宫增重实验，对卷柏、松针、益母草、石胆草及贯众等5种中药的植物雌激素作用进行了研究，以期为临床防治更年期综合症等提供准确、科学的实验基础。

Associated Press sources, we had received from our reporter that where the team settled down is the dormitory of "Omar al-Bashir Tractor Factory", a Chinese-funded factory in El Fasher. When our reporter tried to contact with the factory, they said they know nothing about the exactly information of the team,they allowed them in after checking their Tourism companies and tourist Visa.

美联社消息，该小队下榻地是一间位于法希尔的名为&奥马尔·巴希尔拖拉机厂&的中资工厂的宿舍，当记者致电该工厂时，该工厂称并不知道小队的确切来历，仅是查看了他们的旅游公司证明和旅游签证后便同意其入住。

By reporter gene assay, it was found that both biochanin A and hesperitin activated the activity of CBF1-dependent reporter gene.

藉由报导基因分析，可评估不同药物对於细胞中内生性Notch讯息传导路径的影响，本实验室发现biochanin A与hesperitin可以活化内生性Notch讯息传导路径。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。