查询词典 replicons

In previous studies, during replication of the Streptomyces linear replicons the discontinuous synthesis of the lagging strands will leave a gap on the 3' end of the DNA.

先前的研究发现当链霉菌进行线型DNA复制时，由於在lagging strand的非连续性复制导致在3'端留下一缺口。

Newly-synthesized DNA strands from adjacent replicons are ligated together.

来自邻近的复制子的新合成的DNA链又连结在一起。

Then the HCV subgenomic replicon cell model was incubated with Napp at concentration of 1mg/ml, 10~(-1)mg/ml, 10~(-2)mg/ml, 10~(-3)mg/ml, 10~(-4)mg/ml in cell culture solution and the negative and positive controls were set up correspondingly. After 3 days, the intracellular RNA of HCV subgenomic replicons were detected by FQ-RT PCR.

然后分别取终浓度为1mg/ml、10~(-1)mg/ml、10~(-2)mg/ml、10~(-3)mg/ml、10~(-4)mg/ml的Napp与含HCV亚基因复制子细胞培养液中共同孵育，同时设空白对照组和IFN-α2b阳性对照组，3天后用荧光定量PCR法检测HCV复制子中RNA的含量。

To rely on stretches of DNA in which several replicons can be seen to be active, presumably captured at a stage when all have initiated around the same time, but before the forks of adjacent units have met.

区分独立复制子与熔化眼的常用方法是依靠DNA的伸直，因为在其中几个复制子能够发现具有活力，假设当所有的复制子在相同时间内已经起始时在同一状态时被观察，但必须在连接单位的复制叉相遇之前。

The stability of exogenous plasmids introduced in BMB171 was correlated with the patterns of replicons, and sizes of plasmids.

用pHT3101、pBMB3305、pBMB1736、pBMB671、pBTL-1和pHV1249等6种外源质粒电转化无质粒突变株BMB171的转化频率，分别是Bt-4Q7、Bt-4D10和Bti。

Luciferase activities were measured 72 hr post-transfection. All three mutants showed higher luciferase activity than that of the wild type, and the V85I mutant showed the highest activity. This result was also confirmed by neomycin gene-containing replicons with same mutations.

荧光素酶活性在转染后72小时检测。3种突变子均显示荧光素酶活性高于野生型，V85I突变子显示最高的活性。

In order to confirm the influence of HCV persistent infection on IRF and type I interferon, HCV replicon cell (Huh7 cell line harboring subgenomic HCV replicons) was used.

结果发现，在NDV攻击下，Replicon细胞的IRF-7 promoter活性、内源性IRF-7和IFN-α表达水平均较Huh7细胞低(IRF-7 promoter活性上调倍数：2.54 vs 4.20，P＜0.001；内源性IRF-7mRNA表达水平：0.137 vs 1.671，P＜0.001；内源性IFN-αmRNA表达水平：0.246 vs 2.207，P＜0.001)。

The 329 bp terminal inverted repeat sequence can't form the conserved fold-back secondary structure as that of many Streptomyces linear replicons. Lacking of typical Streptomyces tap/tpg locus for te-lomere replication, pPR2.3c encodes a protein with two domains resembling the telomere associated protein of Strepto-myces and helicase of Haemophilus respectively. No typical Streptomyces iteron-rep locus for replication from the cen-trally located origin, two DNA fragments containing almost all pPR2 were cloned and introduced by transformation into S.

其端粒末端反向重复序列的长度为329 bp，不能像多数链霉菌的线型质粒那样能形成保守的&折返&的二级结构。pPR2虽然没有参与链霉菌端粒复制的保守的tap/tpg基因，但是pPR2.3c基因编码了一个双结构域蛋白，分别同链霉菌的端粒复制相关蛋白Tap和嗜血杆菌的解旋酶具有相似性。pPR2缺少典型的链霉菌重复序列-复制基因区段，将几乎覆盖全长pPR2的两段DNA进行克隆后，不能转化变铅青链霉菌。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。