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The infectivity of porcine endogenous retrovirus with porcine skin fibroblast cells in vitro and in vivo were demonstrated in this study, and there is no evidence of active viral replication of PERV in vivo.

本实验证实了猪皮肤成纤维细胞PERV的体外感染性和在小鼠体内的感染性，但未能找到PERV在体内活跃复制的明显证据。

Secondly, to increase the replication accuracy of every servo axis and to combine with the feature that the machining contour is known in advance, a limited bandwidth zero phase lag desired model is presented in this dissertation.

本文首先综述了国内外超精密机床及其各种伺服控制策略的发展概况，并对数控系统的发展和研究现状进行了比较全面的总结。

The results from the digestion by restriction enzymes showed that there were less EcoR I sites in genomic DNA than in that of Bombyx mori nuclear polyhedrosis virus. It suggested that there might be a different viral replication mechanism in ApNPV life cycle.

结果表明，在ApNPV基因组中只含少量的EcoR I位点，与家蚕核型多角体病毒差别很大，并提示这两种病毒的复制机制可能不同。

The process of invasion and replication of Bombyx mori cytoplasmic polyhedrosis virus in the silkworm, Bombyx mori was studied. After molting, the third instar larvae of B.

采用在家蚕活体内研究家蚕质型多角体病毒的入侵增殖复制过程，探讨BmCPV的入侵过程和增殖复制机制。

Embryonic stem cells and other primitive stem cells of mice have been known for more than 30 years to potently block retrovirus replication.

对于小鼠胚胎干细胞及其他原始干细胞具有潜在阻止逆转录病毒复制作用的了解已经超过30年。

According to the performance requirements of distributed applications, the load balancing is introduced into fault tolerance in this paper to reach tradeoff between active replication algorithm and primary backup algorithm.

针对分布式应用的性能要求，引入了负载平衡机制，以便对activere plication和primary backup容错算法进行权衡。

Perhaps accurate replication at this stage is more easily attained because of this primitivity, and, therefore, tolerance for variance, although low, is not yet a critical problem.

或许精确的仿制，因为其原始性，所以在这个阶段较容易达成，因此，对於变异的容异，尽管不高，也还不是个严重的问题。

Methods: the hbv transgenic mice were generated by microinjection of 1.3 copies hbv genome into the pronucleus of fvb/n zygotes. the pcr assay,elisa,rt-pcr and immunohistochemical methods were used to detect the hbv integration,replication and expression in the transgenic mice.

采用受精卵显微注射法，制备hbv转基因小鼠，用pcr，elisa，荧光定量pcr和免疫组化的方法研究hbv基因在转基因小鼠体内的整合、复制和表达情况。

Fluorescence in situ hybridization was carried out to confirm the integration of HBV DNA into male pronucleus and its replication with cell division in embryonic development.(Reverse transcriptase polymerase chain reaction,RT-PCR) and immunofluoresence assay were performed to observe the expression of the HBV gene in two-cell stage.

材料与方法：成熟雄鼠麻醉后双侧睾丸注射经脂质体DOSPER包裹的HBV质粒，手术后雄鼠与超排雌鼠合笼交配，用荧光原位杂变(Fluorescence in situ hybridization，FISH)分别检测单细胞胚和二细胞胚间期核中HBV DNA的存在与复制，用逆转录聚含酶链反应(Reverse transcriptase-polymerase chain reaction，RT-PCR)和免疫荧光方法检测HBV基因在二细胞胚胎中的表达。

The Ids RNA or siRNA obtained by said invention is mixed with hepatitis B virus expression vector in proper proportion, and injected into the mouse body by vane caudalis hydraulic pressure method, and its result shows that the non-homologous sequence IdsRNA and siRNA basically do not inhibit the replication of hepatitis B virus in mouse body, and these RNA molecules are mixed with hepatitis B virus gene.

将本发明得到的ldsRNA或siRNA以适当的比例与乙型肝炎病毒表达载体混合，并用尾静脉液压法注射到小鼠体内。结果显示非同源性序列ldsRNA和siRNA基本不抑制乙型肝炎病毒在小鼠体内的复制，而与乙肝病毒基因同源性的ldsRNA和siRNA可以有效抑制乙型肝炎病毒在小鼠体内的感染复制，抑制率高达90％以上。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。