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The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因,并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列,通过对克隆的降钙素序列的比较研究,结果显示同一科的鱼降钙素序列保守性较高,同时,根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究,结果显示在分类学上,分类地位较远的鱼,其降钙素相似性较低,分类地位越接近的鱼,其降钙素相似性越高;我们利用谷胱甘肽S-转移酶(Glutathions S-transferase,GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究,应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索,并将纯化的融合蛋白作为抗原,获得了高效价的兔抗鲑鱼降钙素免疫血清;生物活性研究表明,大肠杆菌表达的融合蛋白具有显著的降血钙作用。

Based on the evaluation system, the plants whose relative remediation age was between 0~10 years were named as"Hyperaccumulator"and those between 10 ~ 50 years were named as"Accumulator".7 In the experiments six kinds of substrates were mixed with different solid wastes and the absorption and enrichment of plants on heavy metal Cd was studied. The results showed that relative remediation age was an ideal index to evaluate the capability of the phytoremediation on heavy metals, because it took into account more factors which affected the absorption evaluation effects than others, such as the heavy metal content and the biomass of the over-ground part of plants, the heavy metal content and the whole gross in substrates, harvesting frequency of plants and the environment standard of heavy metal. This made relative remediation age be suitable to the sorption capability evaluation of not only different plants on one heavy metal but also one plant on different heavy metals.8 It can be drawn from the relative remediation age of heavy metal Cd that the absorption capability of different plants was not same.

以相对修复年限为评价指标定义相对修复年限为0-10年的植物为"超富集植物",10-50年的植物为"富集植物"。7在由不同废弃物按一定的比例混合配制的六种土地复垦基质上,系统地研究了几种植物对重金属镉的吸收和富集情况,结果表明,相对修复年限既适用于不同植物对同一种重金属元素的吸收能力的评价,又适用于同一植物对不同重金属元素的吸收能力的评价,是衡量重金属元素植物修复效果的理想指标。8从不同植物对同一基质重金属镉的吸收和富集能力看,不论是经过多年种植的基质,还是第一次种植的基质,小白菜和油菜都是相对理想的植物;而黑麦草、高羊茅和苜蓿的吸收效果并不理想。

Using different concentrations of PEG solution simulated drought stress, measured PB86, PR107, RRIM600 and GT1 rubber tree seedling leaves relative water content, the relative conductivity, malondialdehyde, soluble sugar content, superoxide dismutase, 8 physiological indicators, and their changes in the law, and using principal component analysis, membership function analysis, correlation analysis grey mathematical statistical methods, such as the evaluation of four strains of rubber tree seedlings and with the drought resistance the relevance of indicators; at the same time analysing the use of hypertonic solution screening rubber tree seeds and the drought resistance of different rootstocks on 7-33-97 budding seedlings of the impact of drought that rubber tree planting material and rootstocks drought assessment, and provide a theoretical basis choice.

利用不同浓度的PEG溶液模拟干旱胁迫,测定了PB86、PR107、RRIM600和GT1橡胶树实生苗叶片相对含水量、相对电导率、丙二醛、可溶性糖含量、超氧化物歧化酶等8项生理指标,研究了它们的变化规律,并利用主成分分析、隶属函数分析、灰色关联分析等数理统计方法评价了4个品系橡胶树实生苗的抗旱性及其与各指标的相关性;同时分析了利用高渗溶液筛选的橡胶树种子的抗旱性以及不同砧木对热研7-33-97芽接苗抗旱性的影响,以为橡胶树抗旱砧木和种植材料的评价、选择提供理论依据。

However, unlike the light source relative to the rest of the observer to see is the colorless astigmatism, and the light source relative motion of the observer to see is the red-shift light ( achromatous astigmatism occurs redshift of red-shift light ); Although the different inertial reference frame of the observer can see the speed of light, after the Lorentz transformation is the same as after c; but a different observer are exposed to the light energy is not the same achromatous (astigmatism energy and achromatous astigmatism occurs after the red-shift of red-shift of the light energy is out of the same ).

但是,与光源相对静止的观察者看到的是无色散光,而与光源相对运动的观察者看到的是红移光(无色散光发生红移后的红移光);虽然,不同惯性参照系的观察者看到的光速,经过洛伦兹坐标变换后都一样为c;可是不同的观察者所承受到的光能量是不相同的(无色散光的能量和无色散光发生红移后的红移光能量是完全不相同的)。

The yield, chemical components and relative contents of essential oil extracted from Eupatorium adenophorum Spreng by Soxhlet extraction and steam distillation were compared. The volatile chemical constituents of essential soil extracted were analyzed by Gas Chromatography-Mass Spetrometry. The results showed that the constituents of the volatile from the same plant and the relative contents were significantly different due to different extraction methods.

采用索氏提取器及水蒸气蒸馏两种方法对采于不同时间、不同产地的紫茎泽兰Eupatorium adenophorum Spreng挥发性成分进行了提取,比较了两种方法的得油率、化学组成及其相对含量;采用GC-MS联用技术对其中的挥发性成分进行了分析。

Methods The experiment rabbits were divided into 4 groups. Each group was exposed to different conditions for 120 minutes:normal control group(Td 24℃ 1℃,relative humidity 55% 5%),wound stress group(right crura smashing fracture,Td 24℃ 1℃,relative humidity 55% 5%),heat stress group(Td 38℃ 1℃,relative humidity 65% 5%),wound and heat stress group (Td 38℃ 1℃,relative humidity 65% 5%,right crura smashing fracture).

(1)常温对照组置于干球温度(24 1)℃、相对湿度(55 5)%环境2h;(2)常温创伤组置于同等环境;以350牛顿的力撞击致兔右后肢股骨中下段粉碎性骨折2h;(3)湿热无创组置于仿真模拟气候舱,干球温度(38 1)℃,相对湿度(65 5)%环境2h;(4)湿热创伤组同时施加(2)、(3)两种条件2h。

By means of the comparative analysis in the light of historical changes between China and the foreign ones, the author considers that their similar aspects are that the supply of the relative institutions both reflects the demand regulating the relative redistributive economic relations intervened by state appropriately, both reflects a kind of legal adjustments for the relative redistributive economic relations; the relative legal institutions are paid more attentions to generally in all countries; the succored objects are all distinguished into common ones and special ones generally; citizens receiving social assistance experience the changes from bounty to right generally; social assistance undergoes the development style from negative one to positive one and the changes from free burdens wholely to burdens of some duties;the functions of nongovernmental strength in social assistance are paid more attentions to generally.Their different aspects are that the patten option of distinguished institutions for the urban and rural areas is different;the special emphasis on the contents of social assistance is some different;the level of legislation for social assistance is different;the purpose and the guiding ideology of the design for the social assistance legal institutions are different.

通过对中外社会救助法律制度历史变迁视角的比较分析,认为二者的相近之处表现为社会救助法律制度的供给都体现出规制国家适度干预相关再分配经济关系的需求,都体现为对相关再分配经济关系的一种法律调整,在各国受到普遍重视,其救助对象都区分为一般对象和特殊对象,民众接受社会救助普遍经历了由恩惠到权利的变迁,社会救助普遍经历了由消极被动到积极主动模式的发展,由完全无偿向一定义务负担的转变,民间力量在社会救助中的作用普遍得到重视;差异之处表现为城乡分制模式选择不同,社会救助内容侧重点有所不同,社会救助的法治化程度不同,社会救助法律制度设计的目的存在区别,社会救助法律制度设计的指导思想不同。

The results of measuring chromosome and one-way ANOVA analysis showed that the relative length of all autosomes was different among the sheep breeds.The X chromosomes were similar and the Y chromosomes were different not only in relative length p

测量染色体长度,计算相对长度,并进行单因素方差分析表明,绵羊各常染色体的相对长度之间存在一定的差异;对性染色体进行Duncan′s 多重比较,种群间X 染色体差异较小;Y 染色体种群间不但存在形态差异,且在相对长度上差异极显著P

The seed embryoes were penetrated by 〓 ion beams of 75 MeV/u and the seed endosperms were implanted by the proper degraded 〓 ions, and soluble protein of the seedling in M1 generation was divided into 5 sections to analyze their changes at different molecular weight. 1 In comparison with control, the relative content of the second section (molecular weight is heavier in all sections) of all irradiated materials got down, while the relative content of the fifth section (molecular weight is the lightest) of those went up. 2 The penetrating seeds made the relative content of the first section (molecular weight is the heaviest) and the third section (mid-molecular weight) drop, while the fourth section went up. Moreover, the changes of the third section and the fourth section of the penetrated materials were clearly different from those of the implanted materials. 3 The difference between implanted embryo and implanted endosperm existed in deeper dropping of the first section and higher rising of the fifth section on relative content of soluble protein of the latter. 4 On irradiated materials at lower doses, there were exceptional changes of soluble protein, it may be thought that there was excited effect of low dose.

1.75MeV/u 〓O〓离子经过适当降能后注入小麦种子的不同部位和贯穿整个种子,对其M1代幼苗的可溶性蛋白质组分以及电泳中不同区段蛋白质组分相对含量加以分析,结果表明:1)同对照相比,随着辐照剂量的增加,所有辐照材料第二区段蛋白质组分的相对含量下降;而第五区段蛋白质组分的相对含量升高。2)种子的贯穿处理,同时也引起第一区段和第三区段蛋白质组分相对含量的下降以及第四区段的升高;其中第三、四区段的变化明显区别于注入效应。3)注入胚和胚乳的区别在于后者第一区段蛋白质组分相对含量的较大下降和第五区段的较大提高。4)小剂量辐照的材料,可溶性蛋白质组分的变化异常,可能与低剂量辐射兴奋效应有关。

Based on the evaluation system, the plants whose relative remediation age was between 0~10 years were named as"Hyperaccumulator"and those between 10 ~ 50 years were named as"Accumulator".7 In the experiments six kinds of substrates were mixed with different solid wastes and the absorption and enrichment of plants on heavy metal Cd was studied. The results showed that relative remediation age was an ideal index to evaluate the capability of the phytoremediation on heavy metals, because it took into account more factors which affected the absorption evaluation effects than others, such as the heavy metal content and the biomass of the over-ground part of plants, the heavy metal content and the whole gross in substrates, harvesting frequency of plants and the environment standard of heavy metal. This made relative remediation age be suitable to the sorption capability evaluation of not only different plants on one heavy metal but also one plant on different heavy metals.8 It can be drawn from the relative remediation age of heavy metal Cd that the absorption capability of different plants was not same.

以相对修复年限为评价指标定义相对修复年限为0-10年的植物为&超富集植物&,10-50年的植物为&富集植物&。7在由不同废弃物按一定的比例混合配制的六种土地复垦基质上,系统地研究了几种植物对重金属镉的吸收和富集情况,结果表明,相对修复年限既适用于不同植物对同一种重金属元素的吸收能力的评价,又适用于同一植物对不同重金属元素的吸收能力的评价,是衡量重金属元素植物修复效果的理想指标。8从不同植物对同一基质重金属镉的吸收和富集能力看,不论是经过多年种植的基质,还是第一次种植的基质,小白菜和油菜都是相对理想的植物;而黑麦草、高羊茅和苜蓿的吸收效果并不理想。

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