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The results showed:(1) Chronic cigarette smoking down-regulated the protein synthesis and mRNA expression of BKca and Kv1.5 in bronchial and bronchiolar smooth muscles.

结果显示：(1)慢性吸烟可降低大鼠大气道和小气道BKca和Kv1.5蛋白和mRNA表达。

Sequence analysis showed that BnCOR14 had higher similarity with cold-regulated proteins from Arabidopsis thaliana and Capsella bursa-pastoris and with the typical motifs of late embryogenesis abundant proteins,implied that BnCOR14 might have similar functions possessed by other COR proteins in increasing freezing tolerance of plants.

序列分析表明BnCOR14与拟南芥及荠菜等COR蛋白具有较高的相似性，且BnCOR14具有典型的LEA蛋白序列特征，表明BnCOR14可能在油菜抵抗冷胁迫的过程中具有重要的作用。

And proved that there is CBF gene expression-regulated pathway and transcriptional cascade leading to the expression of cold-responsive genes under cold stress in Capsella bursa-pastoris, which Cbice53 activate the expression of Cbcbf and Cbcbf activate transcription of Cbcor15b and the CbLos2 had a indirect and important regulatory function in controlling cold-responsive genes expression under low temperature like the same pathway in Arabidopsis thaliana.

证明了在荠菜中存在Cbice53激活Cbcbf的转录，Cbcbf激活Cbcor15b的转录，同时CbLos2对Cbcor15b的转录有间接的正向调控作用的抗冷胁迫基因表达级联机制，即存在与拟南芥相类似的抗冷胁迫的CBF基因表达调控途径。

However the three mentioned analysis methods has been so far assured only available to high pathogenic pathotype identification, which was probably associated with the high expression of gene related to high pathogenicity, on the other hand, pathogenicity differentiation of Cercospora zeae-maydis was probably controlled by some complex factors regulated by by polygenes.

相比较而言，只有强致病类型菌株利用三种方法进行鉴定有一定程度的相关性，这可能与强致病类型致病相关因子在蛋白质和DNA水平上的表达强度高有关，另一方面可能是有复合因子控制灰斑病菌的致病性分化，这恰好反映出该病菌的抗性遗传是由多基因控制的特点。

Activities of chymase and ACE were tested by radioimmunological method. Expressions of phosphoric extracellular signal regulated kinase 1/2 (p-ERK1/2) in hearts were measured by western blot. Results: As compared to the control group levels of GSP, myocardial enzymes and myocardial Ang Ⅱ were much lower in those of APS group.

心肌组织的血管紧张素Ⅱ含量；RT-PCR法检测心肌糜酶和血管紧张素转换酶 mRNA表达；放免法检测chymase和ACE的活性；免疫蛋白印迹法检测心肌磷酸化的细胞外信号调节激酶1/2(p-ERK1/2)含量。

Objective To investigate the activation of extracellular signal-regulated kinase and cAMP-responsive element binding protein in the anterior cingulate cortex induced by peripheral injection of formalin.

目的研究外周福尔马林刺激诱导的大鼠前扣带皮层中细胞外信号调节激酶和cAMP反应元件结合蛋白的激活。

The watermelons, the citrullus lamtus or the stems and leaves thereof are taken as raw materials, and the extracting process comprises the following steps: firstly, the raw materials are washed clean and broken to separate the watermelon peel from the watermelon pulp for respectively extracting juice; the two juices are mixed, and micro-filtration and ultra-filtration are carried out to remove insoluble substances and macromolecular substances; and then reverse osmosis and nano-filtration are carried out to obtain the filtrate; the pH value of the filtrate is regulated and then a macroporous strong acid cation exchange resin column is added; and then ammonia water is taken as an eluant for isocratic elution; the eluent obtained is decompressed, distilled, deaminized, discolored, condensed and crystallized to obtain a white crystalline product of the natural L-citrulline extracted from the watermelons.

本发明方法以西瓜、打瓜或者其茎叶为原料，其过程为：首先将原料清洗干净，破碎后将西瓜皮西瓜瓤分开，分别进行榨汁；所得汁液合并，经过微滤和超滤除去不容物和大分子物质；然后再经反渗透和纳滤过滤；透过液调节pH值后通入大孔型强酸性阳离子交换树脂柱；然后以氨水做洗脱剂进行等度洗脱；将洗脱液减压蒸馏脱氨，脱色，浓缩结晶，得到西瓜提取天然L-瓜氨酸白色结晶产品。

Further, with a semi-quantitative RT-PCR technique, the temporal expression of UuMAPKKK-like in U. unicinctus coelomic fluid cells was measured after stimulated by sulfide. The mRNA transcript of UuMAPKKK-like was low in the control and short time stressed (2 h, 6 h) groups, up-regulated gradually after 12h stimulation, and then reached its maximum level at 48h.

进一步采用RT-PCR技术对其在硫化物刺激前后的表达进行了检测，结果显示该基因在对照和应激后2h、6h的个体中表达较弱；刺激12h后表达量增高，并随应激时间增加，呈明显上调趋势。

Methods coix seed and other fifteen chinese medicinal materials were chosen according to the regulated dosages and solved with water for the preparation of tongfengning pill. according to the diagnostic criteria, tongfengning pill was used to treat 98 patients with gouty arthritis and its clinical efficacy was observed.

方法按规定剂量取薏苡仁等15种中药水乏制成痛风宁丸，选择符合诊断标准的患者98例进行临床治疗观察。

Government has an important role to play in fostering this openness - hong kong , like other developed economies , must continue to ensure that its financial markets are well regulated , and that its goods and services markets are truly open and competitive , not cartelized and collusive

政府在培养这种开放环境的工作中，可以发挥重要的作用。香港，正如其它发达的经济地区一样，必须继续确保规范金融市场、真正开放物资及服务市场，公平竞争，而非垄断和欺诈。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。