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When 3 mmol·L-1 crocin treated T24 cells for 48h, the difference was significant compared with the control group ( P .05). Crocin induced wide changes of the gene expression profile of T24 cells. A total of 836 genes were up-regulated or down-regulated by more than 2 times, which were involved cell cycle controlling, DNA cell apoptosis, replication factor, and so on.

基因芯片检测发现3 mmol·L-1藏花素作用T24细胞48 h引起该细胞系基因表达谱广泛地改变，其中表达差异2倍以上的基因共836个，这些差异表达基因的功能涉及多个方面，最为明显的是与细胞生长调节相关的细胞周期调节基因、细胞凋亡调控基因、DNA复制相关基因等类型。

Crocin could inhibit the growth of BALB/c xenograft tumor. The morphology changes of cell apoptosis were observed. Bcl-2, Cyclin D1 and survivin expressions determined by immunohistochemical staining were down-regulated after treatment with Bax expression up-regulated.

Crocin对人膀胱癌裸鼠移植瘤的生长具有抑制作用，电镜观察可见细胞凋亡的形态学改变，免疫组化结果显示，经Crocin处理后Bcl-2， survivin，Cyclin D1的表达下调，而Bax的表达上调。

Result In the HepG2 cells transfected by pSliencer 3.1-H1 neo-shTERT, expressions of hTERT, Bcl-2 and mitochondrial cyt C were significantly down-regulated, while Bax and cytoplastic cyt C were obviously up-regulated, and active caspase-9 was found in addition to procaspase-9 compared with that in negative control cells and untransfected cells.

结果 与未转染细胞和对照细胞相比，转染细胞hTERT、Bcl-2和线粒体cyt C表达明显减少，Bax和胞质cyt C表达明显增加；未转染细胞及对照细胞仅见约46kD的前caspase-9条带，转染细胞可见46kD前caspase-9条带和35kD caspase-9 p35亚基条带。

FCM analysis showed that the apoptotic rate of MDA-MB-231 cells and intracellular Ca(superscript 2+) concentration increased gradually with the increase of deguelin concentration. RT-PCR and Western blotting analysis showed that the expression of Bcl-2 mRNA and protein was clown-regulated and that of Sax was op-regulated after deguelin treatment.

流式细胞术检测显示细胞凋亡率和细胞内钙离于浓度随鱼藤素浓度的增加而增多；RT-PCR和蛋白印迹检测结果发现鱼藤素能使Bcl-2 mRNA和蛋白表达减少，而Bax表达增加。

Flow cytometry results showed that: 1） When cells were treated with Diallyl trisulfide at the concentration of 10 μg/mL for 12 h and 24 h the percentage of G0/G1 phase cells was decreased and that of G2/M phase cells was significantly increased compared with those in the control group.2） Diallyl trisulfide down regulated Bcl expression while up-regulated bax expression.

结果：MTT显示大蒜素能抑制人结肠癌 HT29细胞的增殖，并具有时间-剂量依赖性；流式细胞术测定结果显示10 μg/ml大蒜素可明显诱导HT29细胞凋亡，并将细胞周期阻滞在G2/M期；可降低癌基因蛋白Bcl表达，而促进Bax表达。

Combining SSH and cDNA microarray to screen of up-regulated ESTs in the water stress Erianthus arundinaceus based on the analysis of up-regulated ESTs,a full-length cDNA sequence was cloned from sugarcane through Rapid Amplification of cDNA Ends method,and the gene expression character was analyzed by real time RT-PCR.

应用消减文库技术结合cDNA芯片技术筛选水分胁迫诱导的基因的EST序列，根据筛选到感兴趣的上调表达基因的EST序列，用RACE技术获得SSADH的全长cDNA序列，并通过实时荧光RT-PCR技术对该基因的表达特征进行分析。

Constitution litigation should follow balanceable rule and buck for theharmoniousness of the inerratic method regulated by rule andnon-inerratic method regulated by non-rule.

宪法诉讼在型构社会秩序时应遵循平衡的准则，而且，还应谋求由规则调整而形成的规则秩序与由非规则支配所调整的非规则秩序之间的和谐。

All the issue is not regulated or not fully regulated by law,however we can frequently face the issue in the juridic practice.

这些问题又恰恰是法律所未规定或规定不全面而为司法实践所常见者。

Results: Twenty one differential protein spots were screened and 5 differential proteins were identified. They included Ezrin protein, transcription elongation factor A, and lamin A/C which were up-regulated in LoVo cells and non-metastatic protein expressed in isoform a (NM23 A) and heat shock protein 8 (HSPA8) which were up-regulated in SW480 cells.

结果：筛选出21个差异蛋白质点，成功鉴定出5种差异蛋白质，其中在LoVo细胞株中表达上调的蛋白有埃兹蛋白、转录延长因子A (transcription elongation factor A)和甘氨酸A/C，在SW480细胞株中表达上调的蛋白有NM23蛋白[non-metastatic cell 1 protein (NM23A) expressed in isoform a]和（heat shockprotein 8，热休克蛋白8）。

In addition, the up-regulated expression of hypothalamic neuropeptide Y and the up-regulated lipopexia could be decreased significantly by acupuncture

针刺治疗肥胖效果显著，且显著降低肥胖大鼠升高的下丘脑NPY含量，减少肥胖大鼠已增加的脂肪蓄积量。

Nowadays, most of research are to build a transmutative Petri Nets through adding controlling place sets, controlling arc sets and controlling policy to the basic Petri Nets, while the Controlled Petri Nets could be used to argue many controlling theory problems conveniently and to induce many logically and physically supervisory and solve the Event Feedback Controlling Problems and State Feedback Controlling Problem in DEDS supervisory theory.

目前大多数的研究表现为在变形后的受控Petri网基础上，利用各种方法求得各种逻辑型、结构型控制器，解决DEDS监控理论中的事件反馈控制问题与状态反馈控制问题。

On one hand, there are discussions with the works council and union about extension of short time working up to the end of September.

一方面，有讨论，工程理事会和联盟关于延长工作时间短至9月底。