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So in this paper based on the characteristics and the optimum state theory of the vehicle, some kinds of control schemes are compared and discussed, and the fuzzy PID control scheme is adopted at last. The relation between air-cushion pressure and fan rotational speed on the optimum driving state is studied. The cushion pressure is used as controlled variable and the fan revolution is used as regulated variable. And transducer and fan motor are actuators.Based on the structure and operating properties of half-tracked air-cushion vehicle, the control system scheme and structure are presented. A control scheme using the self-tuning PID fuzzy logical controller is adopted. Fuzzy rules are set up and some researches have been done for fuzzy tuning method. At the same time the real-time measurement scheme by computer control is proposed, and electric circuits are designed. In the control system of half-tracked air-cushion vehicle cushion pressure P〓 is used as controlled variable and the fan speed n〓 as regulated variable.

因此本文以半履带式气垫车的特性和最佳工作状态理论为指导，通过对各种控制方法的比较与探讨，提出采用模糊PID控制方案，并且研究了在最佳工作状态下垫压及风机转速的关系，提出气垫压力作为控制参数，变频器和风机电机为执行机构，风机转速为调节量；主要进行了自整定模糊PID控制器的设计，研究了自整定PID模糊控制器的实现方法，编制了模糊控制规则，制定了模糊控制参数查询表，并分析了影响自整定模糊控制器相关的影响因素，确定了模糊控制表；为了实现半履带式气垫车的自动控制，还提出了计算机模糊控制系统的原理与结构，设计了相应的测量电路与控制软件。

Results 2 ClAdo and 2 CldAdo had different cytotoxicities on the cells, while 2' dAdo had no cytotoxicity on the cells. 2 CldAdo blocked the cell growth at the phase of S and G2/M, while 2 ClAdo at the phase of G0/G1. 2 CldAdo mainly induced cell necrosis, while 2 ClAdo induced both cell necrosis and apoptosis. Down regulated Bcl 2 expression and up regulated p53 and Bax expression were associated with 2 ClAdo, while 2 CldAdo down regulated Bcl 2 expression and up regulated mildly p53 expression without any effect on Bax expression.

结果 2 ClAdo、2 CldAdo对所研究的细胞均有程度不等的毒性；2' dAdo对各细胞生长、增殖均无明显抑制（c=100 μmol/L即30 μg/mL内）；2 CldAdo明显阻断细胞于S、G2/M期，2 ClAdo阻断细胞于G0/G1期；2 CldAdo 致细胞坏死为主，2 ClAdo 既致细胞坏死又诱导凋亡；2 ClAdo 下调Bcl 2，上调P53、Bax蛋白表达；2 CldAdo小幅上调P53、小幅下调Bcl 2，不影响Bax蛋白表达。

Results (1) The total RNA, reverse transcription product and fluorescence labeled cRNA were all of high quality;(2) There were 420 up-regulated genes and 552 down-regulated genes among 2-fold DEGs, including DKK1 (dickkopf homolog 1) which was 5-fold up-regulated;(3) The results of real time RT-PCR, using β-actin as an internal control, showed that the 2-ΔCt values of DKK1 in HCC, PCT and NLT were 0.089 504, 0.007 65 and 0.000 631 respectively.

结果 （1）配对组织总RNA质量高，反转录cRNA及荧光标记质量好；（2）2倍差异表达基因中，上调基因共420个，下调基因共552个，其中包括5倍上调基因DKK1；（3）以β-肌动蛋白为内对照的实时逆转录聚合酶链反应结果提示，DKK1在癌、癌旁和正常肝组织中的2-ΔCt值分别为0.089504、0.00765和

In stationary phase, there were 117 genes up-regulated and 109 down-regulated after transfer to 20℃. Up-regulated genes mainly included hypothetic proteins, genes involved in energetic metabolism and substance metabolism, virulence genes. Down-regulated genes mainly included hypothetic proteins, genes involved in substance metabolism and amino acid metabolism.

静止期表达上调和下调的基因分别有117和109个，表达上调的基因主要包括假设蛋白、参与能量代谢和物质代谢的基因、毒力基因；表达下调的基因主要有假设蛋白、参与氨基酸代谢和物质代谢的基因、毒力基因。

The expression of Bax and Bcl-2 in cerebellum cortex and bathypelagic nuclei of the ovariectomized SD rat were up-regulated and down-regulated respectively, and the expression of the 2 proteins was regulated to normal after the rats were treated with 17β-estradiol.

结果显示，摘除双侧卵巢后，SD大鼠小脑皮质及深层核团中Bcl-2的表达有不同程度的降低，而Bax的表达出现不同程度的上升；给予外源性17β-雌二醇治疗后，2种蛋白的比例基本恢复正常。

Of 8000 cDNA elements, custom cDNA array identified 47 as differentially expressed genes/ESTs with above 3-fold changes in the hippocampus 35 days after lesion, in which 25 were up-regulated and 22 down-regulated. Northern blot hybridization indicates that false ratio is high with the cDNA array experiment. Of 6234 rat CNS genes/ESTs, cDNA microarray identified 152 as differentially expressed cDNA clones with changes between 1.5-2.8 fold in the hippocampus 10 days following perforant path injury, 32 were up-regulated and 152 down-regulated.

Custom cDNA array 筛选的8000个cDNA 克隆中有47个在去神经30天海马显示差异表达且表达变化在3倍以上，其中25个表达上调，22个表达下调； Northern blot 实验表明cDNA array 的假阳性率是较高的。cDNA microarray在大鼠中枢神经系统表达的6234种基因和EST序列中，筛选到了152个基因和EST 序列在去神经10天海马差异表达，表达变化在1.5-2.8倍之间，其中表达上调的32个，下调的120个。

Self-regulated writing teaching for keeping a weekly diary is a newly-constructed writing teaching style inspired by the idea of the new curricula ,which attempts to change the monotonous state of being teacher-centred ,the separation of content and form from real life and from the studrnts ' interest and teaching situation. The links of self-regulated weekly diary keeping requires the students to mind themselves and society and the effect of knowledge, ability and virtue will be taken into comprehensive account. And the students are requested to allow full play to their initiative, enrich their emotions throughout the self-regulated atmosphere, accumulate knowledge and improve ability.

自主性周记写作教学正是基于新课程理念的基础上建构起来的一种新的写作教学方式，力图改变传统写作教学中的以教师为中心，内容与形式脱离生活实际，脱离学生兴趣及教学情境单调的现状，在自主性周记写作的各个环节中，它要求学生关注自我，关注社会，综合考虑知识、能力与情操等三方面因素的作用，充分发挥学生的主体性，学生自始至终都在自主的氛围中丰富情感、积累知识、提高能力。

Mechanical strains also regulated the protein and mRNA expression of several differentiation markers, as well as the activation of extracellular signal-regulated kinases, p38 MAP kinase and protein kinase B in a frequency-dependent manner. Furthermore, the inhibition of p38 pathway could block the strain-frequency induced the phenotype modulation of VSMCs, neither ERKs nor Akt. Frequency of mechanical strain, not conditioned medium, regulated the phenotype of VSMCs in a frequency-dependent manner. Rho-GDI alpha was suppressed by the mechanical strain at 1Hz.

采用免疫细胞化学法检测VSMCs形态和排列的变化；RT-PCR和Western blotting检测表型标志分子α-肌动蛋白、肌球蛋白重链(SM1/2)、肌动蛋白相关蛋白SM22α和调宁蛋白(h1-calponin)的mRNA和蛋白水平的变化；抑制剂或RNA干扰阻断可能的信号调节分子的活性或表达，包括p38、细胞外信号调节激酶1/(2extracellular signal-regulated kinases， ERK1/2)、蛋白激酶B和Rho-鸟苷酸解离抑制因子(Rho-guanine nucleotide dissociation inhibitor， Rho-GDI alpha)，研究了不同频率张应变对VSMCs表型转化的影响及其调节机制。

Results showed that the expressions of both Fas and FasL on tumor cells were up-regulated compared with noncancerous liver cells from same patient.When compared with the PBLs from same patient,the expression of Fas on TILs was highly up-regulated whereas the FasL down-regulated.

结果显示，肝细胞癌患者肿瘤细胞Fas与FasL表达水平较自身非肿瘤肝细胞上调；肿瘤浸润淋巴细胞与自身外周血淋巴细胞相比，Fas表达上调而FasL表达下调。

So it is very urgent to set up a useful teaching-model of self-regulated learningThere are two-aspect meanings about the studies:1 to set up the physics teaching-model of self-regulated learning in senior middle-school can promote the research of theory and practice in self-regulated learning, enrich the study content of self-regulated learning and provide a choice for the reform of physics class-teaching method.2 to put the model into practice can raise the students ability of self-regulated learning.

本研究的意义在于： 1结合高中物理教学实际、高中生的心理特点，建立有效的高中物理&自主学习&课堂教学模式，可促进自主学习理论与实践的结合； 2通过对自主学习过程各种因素、各个方面相互关系与协调发展的系统研究，将在一定程度丰富自主学习的研究内容，推动自主学习理论与实践的发展； 3&自主学习&课堂教学模式的实施，将增强学生学习的自主程度，提高自主学习能力，促进学生物理学习方式的转变；并为推进素质教育，改革传统物理课堂教学提供了一条新的途径和选择。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。