查询词典 regular

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

All the Augustinian convents of Northern Germany were, in accordance with this decree, to become parts of the regular observance.

所有的奥古斯丁修道院北德国，根据这项法令，成为部分经常遵守。

This Lieutenant of Police, whose title was changed in 1674 to Lieutenant-General of Police, in addition to his regular duties as a criminal judge, was sometimes required by the king to concern himself with matters gravely affecting the well-being of France and the aureole of glory with which Louis XIV always aspired to surround himself.

这种警察，他地头衔改为中尉在1674年为中将，警察总长，除了他的正常工作作为刑事法官，有时由国王需要关注的茄题厉害影响了自己的良好福利和法国光环与荣耀的路易10四老是渴望包围自己。

Write source code more easily with EditPad Pro's syntax coloring, search and replace with regular expressions, DOS/Windows/UNIX and ANSI/Unicode conversions, running external applications or scripts, persistent and rectangular selections, hexadecimal mode, integrated diff to clearly compare files, auto and block indent, line numbers etc.

写的源代码更容易与editpad亲的语法著色，搜索和替换与正则表达式， DOS的/在Windows / Unix和美国ANSI / Unicode的转换，运行的外部应用程序或脚本，持久性和矩形的选择，十六进制模式，综合差异比较清楚的档案，汽车和块缩进，行号等。

We shall use algebraic methods to study graphs which are highly regular although this regularity is not expressed in terms of the automorphism group.

我们将用代数方法来研究高度正则的图，尽管这种正则性不是用自同构群来表达的。

Regular inspection of sphere tank is very important to ensure tank quality and human security,which includes safety risk identification,safety risk control,and quality control of auxiliary operation.

对球罐定期检验作业进行安全风险识别、风险控制以及辅助工作的质量的控制，是确保球罐定期检验质量及人员安全的重要内容。

An aviator in the regular Navy pipeline would never get that experience," says Storrs."

按照正常的海军途径，一名飞行员将永远不会有这样的经历，"斯托丝说。"

This model is very useful for policy-maker in the determination of whether AVS or regular update is necessary.

这个模型对决定购买防毒软体或更新的决策者是非常有用的。

It has proved that well sets completely satisfy all axioms of ZFC -(the classical system of axiomatic set theory ZFC without the regular axiom).

在中介公理集合论系统中重新定义了良集的概念，讨论了它的性质。

The material's elastic constants for different orientations are obtained from the elastic constants along the axis of symmetry through the regular tensor transformation law.

不同旋转角度时材料的弹性常数是从晶轴坐标系下的弹性常数通过张量变换得来的。

Recall that, in the Kolmogorov axiomatization, measurable sets are the sets which have a probability or, in other words, the sets corresponding to yes/no questions that have a probabilistic answer.

延期开工的Kolmogorov宣布要衡量所有套职能有限许多坐标限制在于衡量子寅。换句话说，如果是/否的问题回答f可看的价值观念在许多最有限坐标，然后它有一个概率的答案。

The experimental results showed that the algorithm can filter singular points and remain fixity of the target edge.

实验表明：基于模糊理论的图像智能滤波方法比常规图像滤波方法运算量小，它既能去除奇异点，又能保持图像中目标的边缘不变性，具有很好的滤波效果。