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The transient transfection efficiency was measured by flow cytometry. The stable transfected cells were screened by G418. Results Electrotransfection had the highest transient transfection efficiency (56.8%), with the most masc clones of stably transfection and the least time taken to form big masc clones (20 days). Cationic polymer mediated transient transfection had the lowest efficiency (1.7%), with the least masc clones and the longest time taken to form the big masc clones (30 days). Cationic liposome transfection had a transient transfection efficiency of 39.9% and took 25 days to form the big masc clones.

结果 电转染有最高的瞬时转染效率(56.8%)，稳定转染得到的阳性克隆最多，且从细胞筛选到扩增至得到阳性大克隆所用的时间也最少（约20d）；用阳离子聚合物瞬时转染率低(1.7%)，稳定筛选同样可以得到阳性大克隆，但是数量很少且从细胞筛选到扩增至阳性大克隆所用的时间也最多（约30d）；阳离子脂质体转染效果居中，瞬时转染率为39.9%，从细胞筛选到扩增至得到阳性大克隆所用的时间约25d。

He used to utilize concise language and plain poetic form: vocabulary in his poems is mainly informal, and most of them belong to monosyllables; on syntax, Frost mainly adopts normal, prosaic lingual orders and strict and orthodox grammars; on the poetic form, he often writes traditional blank verse, sonnet and pays more attention to the phonetic natural rhythm, lines' musical effect and the relations between sound and meaning, which form his unostentatious artistic style.

他的诗歌通常运用简洁凝炼的语言和平淡的诗歌形式：诗歌中出现的大多是口语化的词语，并且大多数词都属于单音节词；在句法方面，大多的句式采用的都是正常的、散文化的语序和严格正统的语法；在诗歌形式上，他主要使用无韵诗和104行诗，他也10分重视语音的自然节奏和诗句的音乐效果以及音与义之间的联系，从而形成质朴无华的艺术风格。

Firstly, sublime expression changed form grand narration code to calm and little-narrativite. The narrative view transferred form king to personal and civilian, thus to look into historical and practical sublime in everyday view, and to transfer to anti-epic in narration, and to establish sublime topic by chanciness explaining inevitability and overthrowing hero mythic. Secondly, flat character as a new form of teleplay sublime expression, break out round character personifications.

首先，崇高的表现范式中庄重的宏大叙事被编码为平实、轻巧的小型叙事，叙述视角从王者视野转向了个人视野、民间视野，以日常观照的方式看取历史与现实中的崇高，叙事方式出现了反史诗性的转向，以偶然性解构必然性和颠覆英雄神话的方式建构崇高主题；其次，扁型人物作为电视剧中崇高表现形式上的新态势，打破了圆型人物作为崇高人格化形象的大一统格局。

Digital Art is created and stored in a non-material form on the computer's memory systems and must be made physical, usually in the form of prints on paper or some other form of printmaking substrate.

数字艺术是建立并储存在一个非物质形式的电脑记忆体系统和必须作出的身体，通常的形式打印在纸上或其他形式的版画基板。

The RhoA oncogene , have been revealed in recent years, are closely related to various malignant tumours. RhoA, belonging to Rho family, is one member of the ras superfamily, whose encoding production, namely RhoA protein, pertaining to micromolecular mass GTP conjugated protein family, in GTP conjunctive active form and GDP conjunctive unreactive form. It exist in the latter form in normal cells and the switch of the two forms enables them to educe functions, paralleling"molecular switch", the diminutive G protein in activated state being able to activate the wink dromo-iter to work functions, which is in the resemblance of process during which the Ras genic mutation induces its continous activations.

RhoA是近年来新发现的与多种恶性肿瘤密切相关的癌基因，RhoA属于Rho亚家族，是ras基因超家族的一员，其编码产物RhoA蛋白属于小分子量GTP结合蛋白族，分为GTP结合的活性型和GDP结合的非活性型两类，在正常细胞内以GDP结合的非活性型存在，两种活性形式之间的转换使他们能够发挥一种类似&分子开关&的作用，活性态的小G蛋白能激活下游的信号传导通路，行使其功能。

This chapter stresses on "the dynamic tests" of internal and external structures, and the internal organization, to express his free and illusive style; Chapter VII:"Musical form structure research". In this chapter the historic exploration and innovation of Scarlatti in musical form structure is a keystone that dramatically contributes to the development of ancient sonata-form.

第七章：&曲式结构研究&，本章重点研究斯卡拉蒂在曲式结构方面富有历史意义的探索和创新，为古典奏鸣曲式的定型发展做出了突出的贡献，这是评价斯卡拉蒂历史地位的一个重要方面。

Despite the assistance, he says civil rights abuses continue to occur, including "voluntary interviews," the Absconder Apprehension Initiative and the Special Registration Program.

尽管援助，他说，公民权利的行为继续发生，包括"自愿采访，"潜逃逮捕倡议，并特别登记计划。

I urge any American living outside the country to get an early start checking his/her registration and receiving an absentee ballot, because it can take a while.

我奉劝身在国外的所有美国人早点着手核对登记表，并接收缺席选举人票，因为这得花点时间。

But even though I registered and downloaded a New Jersey registration at the latter the other day, I still have to mail it back to Essex County, N.J., and wait hopefully for an absentee ballot to arrive the same way -- so I can again use snail mail to cast my ballot.

但是虽然我前几天注册并下载了一份新泽西州的登记表，我还是得把它寄回纽约州艾塞克斯县，同时满期待地等着一张缺席选举人票以同样的方式送到我手中──好让我再次通过奇慢无比的邮寄方式来投下我的一票。

The acceptance of the use of department equipment numbers, the gathering of relevant technical information, the establishment of equipment files and the "account department equipment" classification registration; 4 According to the Treasury "equipment-acceptance" for storage procedures.

使用部门对验收合格的设备进行编号，收集相关技术资料，建立设备档案，并在《部门设备台帐》上分类登记； 4 库房根据《设备验收单》办理入库手续。

In other research, chondroitin is being investigated for use in several other conditions.

在其它研究，软骨素被调查用於几个其它情况。

They would make fun of him for having to work.

他们拿他的工作来开玩笑。