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In order to diagnose disease caused by muscovy duck parvovirus rapidly and accurately, and at same time, further study the taxonomic relationship between muscovy duck parivovirus and goose parvovirus, two pairs of PCR primers (LHMP1/LHMP2 and LHGP1/LHGP2) were designed respectively base on complete nucleotide sequences of Muscovy duck and Goose parvoviruses registered in the GenBank, The LHMP1 for the upper one and the LHMP2 for the lower one, corresponding to the specific nucleotides sequence 454-472 and 1173-1155 respectively in MDPV, and the LHGP1 for the the upper one and the LHGP2 for the lower one, corresponding to the specific nucleotides sequence 454-472 and 1173-1155 respectively in GPV.

为了能快速、准确地对番鸭细小病毒病进行诊断，同时为了能进一步弄清番鸭细小病毒与鹅细小病毒在分类学上是一种怎样的关系，比较GenBank中MDPV和GPV的基因序列，针对这两种病毒左阅读框编码非结构蛋白基因序列的相同区段，分别构建两对PCR引物。第一对引物LHMP1／LHMP2是根据MDPV的基因序列设计的，上游引物针对MDPV基因序列的454～472；下游引物针对基因序列的1173～1155，为19个碱基，扩增跨幅为719bp。

In order to compare the homology of the gene encoding VP2 between MDPV-Q and MDPV which was registered in the GenBank, also in order to find out changes of the VP2 gene and the immuno-genicity between the wild-strain MDPV-Q and the attenuated strain MDPV-26 which derived by continued passage of virulent wild-type in muscovy duck embryo, a pair of primer (LHMP7/LHMP8) was designed. The upper one LHMP7 and the lower one LHMP8 corresponded to the MDPV-specific nucleotides sequence 2885-2900 and 4618-4604 respectively, according to the MDPV nucleotide sequence databases. The length of the sequence embraced by the primers was 1734bp.

为了获取这一基因片段与国外分离株进行同源性比较，同时也为了了解MDPV强、弱毒株VP2基因之间的异同关系，及其免疫原性的变化规律，通过DNA重组技术，设计了一对引物LHMP7／LHMP8，该对引物选取位于2885～2900及4618～4604的两段序列，跨幅为1734bp，并在这两条引物中分别加入两种限制性核酸内切酶SacⅡ和KpnⅠ的酶切位点，分别对MDPV-Q和由该株病毒经人工致弱后得到的MDPV-26株进行PCR扩增，并将PCR产物克隆到pMD18-T载体上，分别得到二个重组子：pMD18-T—M-Q VP2和pMD18-T-M-26 VP2。

In one ward, Fagge A, the PDP, according to one of its operatives, had budgeted 594,000 naira ($4,650) for 21,000 registered voters and 35 ballot boxes.

据为其工作的一位特工介绍，在Fagge A选区，有2.1万注册选民和35个投票箱，人民民主党在那里预算了59.4万奈拉（4，650美元）。

Multiple voters, who will have registered several times with sympathetic election officials, might vote ten times, at a reduced bulk rate of 100 naira—still picking up a tidy 1,000 naira each.

多次选民----富有同情心的选举官员允许他们多次注册----也许投了10张选票，虽然如此一来，一次投票拿的钱降到了100奈拉，但加起来，他们的投票收益也多达1，000奈拉。

A person registered to practice naturopathy was entitled to use the title "Doctor of Naturopathy."

看看吴永志到底拿的是什麼执照，什麼时候拿的「证书」，便知其真假

I learn the yoga from Gurukul kangri university haridwar, indian institute for yoga and naturopathy,200 hours registered yoga teacher from yoga alliance.

我在 Gurukul kangri 大学学习的瑜伽，它是一家瑜伽和自然疗法的印度学院。获得200小时美国瑜伽联盟教练培训证书。

Secondary effects and complications were registered according to NCI CTC classification.

副效应和并发症被登记根据NCI CTC分级。

This method consists of two nested loops: the first loop iterate through all the registered types.

该方法包括两个嵌套循环：首先遍历所有已注册的各类迭代。

Registered capital of 10 million, net fixed assets of about 50 million yuan.

注册资金1000万元，固定资产净值约5000万元。

As a freight forwarder, must understand that the ship features, such as ship registration and the tonnage of the country and, gross register tons, net registered ton, bulk pit, packaging pit, with a total deadweight, load line, the ship class such knowledge.

三、货运代理应了解不同类型的船舶对货主货物的适应性。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。