查询词典 refolding

It is found that the best refolding yield for 0.1g/L linear HNTX-Ⅲ could be obtained in double-distilled aqueous solution at pH 7.5 containing 1.0 mol/L L-arginine, 1.0mmol/L reduced glutathione, and 0.1mmol/L oxidized glutathione.

最佳的氧化复性条件为pH 7.5的重蒸水溶液体系，样品质量浓度为0.1mmol/L，还原型谷胱甘肽和氧化型谷胱甘肽的浓度分别为1.0mmol/L和0.1mmol/L、L-Arg浓度为1.0mol/L。

The subject invention provides innovative methods of manipulating the structure and function of egg proteins, preferably albumen, through controlled acid and alkali unfolding and subsequent refolding.

主题的发明提供创新的方法操纵和蛋白质的结构和功能，最好是蛋白蛋，通过控制酸和碱展开及后续研究。

Coli,-easy proliferation, and another advantage of yeast is the modification and refolding of proteins after expression. But native NGF was difficult to be expressed in yeast cells; the scientist in Japan synthesized a NGF gene by changing the triple-codes into yeast's favorers.

但NGF在酵母表达非常困难，因此日本的科学家利用人工合成的方法合成了一条NGF的基因，并将编码蛋白的三联密码该为酵母嗜好的三联码，成功地在酵母内表达了NGF。

And finally, a suggested refolding mechanism of reduced-denatured egg white lysozymes in urea solution was presented.

最后，给出了一个建议的还原变性蛋白溶菌酶分子在脲溶液中的折叠机理。

Finally, a suggested refolding was separately presented for the reduced and non-reducing egg white lysozymes in the urea solution.

最后，给出了一个建议的还原和非还原蛋白溶菌酶在脲溶液中的重折叠过程。

The aggregation interaction between reduced-denatured egg white lysozymes during refolding procedure in urea solution was studied by means of reducing and non-reducing protein electrophoreses.

通过还原和非还原蛋白电泳，研究了还原变性蛋白溶菌酶在脲溶液中重折叠过程的集聚相互作用。

The refolding of reduced and non-reducing egg white lysozymes in a urea solution was studied by a "phase diagram" method of fluorescence.

用荧光&相图&法研究了还原和非还原脲变性蛋白溶菌酶的重折叠。

This equation was tested by the renaturation processes of denatured egg white lysozyme in guanidine hydrochloride and urea solutions, with the results to show that when guanidine hydrochloride and urea concentrations were separately higher than 1.25 and 3.00 mol/L or separately lower than 1.00 and 3.00 mol/L, the refolding intermediates of egg white lysozymes were more easily aggregated to aggregate state or more easily renatured to native state, respectively.

以蛋白溶菌酶在盐酸胍和脲溶液中的复性过程对此关系进行了验证，结果表明，当溶液中盐酸胍和脲浓度分别大于1.25 mol/l和3.0 mol/l或分别小于1.0 mol/l和3.0 mol/l时，它们的折叠中间体分子分别更倾向于集聚成集聚体或更倾向于复性成原始态分子。

The results of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L,there existed some stable hydrophobic regions,which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid,in the partially folded intermediate of Bacillus amyloliquefaciensα-amylase;with the denaturation concentration increasing,the stable hydrophobic regions disappered.the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed that using acrylamide as quenchers,with the protein denaturation extent increasing,the number of Trp that can be quenched increased untill all the Trp residues were quenched;Using potassium iodide as quenchers,with the maximum number(8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciensα-amylase molecule could be quenched by potassium iodide;with the denaturation concentration increasing,the number of Trp that can be quenched decreased to 5.the results of their protein electrophoreses and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciensα-amylase formed during the whole unfolding/refolding procedure of Bacillus amyloliquefaciensα-amylase induced by guanidine hydrochloride or urea.

ANS外源荧光探针结果表明：盐酸胍诱导的芽孢杆菌α-淀粉酶分子去折叠过程中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域；而随着芽孢杆菌α-淀粉酶分子在盐酸胍溶液中变性程度的加深，这一疏水区域逐步被瓦解。丙烯酰胺和碘化钾猝灭结果表明：在盐酸胍溶液中，随着芽孢杆菌α-淀粉酶分子变性程度的进一步加深，其分子内能够被丙烯酰胺接近的色氨酸残基逐渐增多，直至全部被猝灭。但位于芽孢杆菌α-淀粉酶分子表面的能够被碘化钾猝灭的色氨酸残基，在中间态芽孢杆菌α-淀粉酶分子中数目达到最大的8个，而随着其分子变性程度的进一步加深，反而减少至5个。

By constructing a residue-residue contact matrix, using correspondence analysis, and then selecting optimal partition function of a protein according to refolding free energy and some empirical scoring functions, a new computer program, PDOM, was developed, which was applicable to both continuous and discontinuous domains. When compared with the manual partition results reported by crystallographers, PDOM has achieved an accuracy of 76% on a test data set including 55 protein structures frequently used.

现在，作者通过构造氨基酸残基相互作用矩阵，并进行对应分析，然后根据去摺叠自由能和一些经验打分函数对蛋白质进行切割和优先，发展了可以同时处理连续和不连续结构域的划分方法。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失