查询词典 refolding

A potential approach to this problem is to make use of a natural defense mechanism called " the stress response," which is characterized by a transient down - regulated synthesis of nonvital cellular proteins and increased production of endogenous protective factors known as heat shock proteins that act to allow proper refolding of proteins denatured by the incipient injury.

应激反应的特征是一过性下调细胞非必需蛋白的合成及增加被称为热休克蛋白的内源性保护因子的合成。热休克蛋白作用是促使损伤导致变性的蛋白适当地再折叠。HSP家族中HSP70被认为在细胞保护中发挥重要作用。

In accordance with the invention, the hG-CSF protein can be prepared with high purity through rather simple process facilitating secretion of large amount of hG-CSF fusion protein into the periplasm, which does not require complicated processes such as solubilization and subsequent refolding required for isolation of the hG-CSF protein produced in cytoplasm as insoluble inclusion bodies by conventional techniques, thus, the hG-CSF protein can be widely used as an active ingredient in the development of supplementary agents for anticancer therapy.

根据本发明，通过促进大量hG－CSF融合蛋白分泌到周质中的相当简单的方法可以高纯度制备hG－CSF蛋白，所述方法不需要复杂的加工过程例如对于通过常规技术分离胞质中产生的作为不溶性包函体的hG－CSF所需的溶解和随后再折叠，因此，在开发用于抗癌治疗的补充药物的过程中可用hG－CSF蛋白广泛地作为活性成分。

2 Is a good model for studying dissociation and reassociation during unfolding and refolding.

肌酸激酶是研究蛋白质去折叠和再折叠过程中解聚和再聚合的模型蛋白之一。

The results showed that rate limiting step during reassociation of creatine kinase subunits existed in the refolding pathway under the various conditions.

结果显示肌酸激酶再折叠过程中，亚基的再聚合是限速步骤。

An optimal refolding procedure has been developed to reford active proteins from inclusion bodies.

故欲发展出一个最适的复性程序，将包涵体回复成具有活性的蛋白质。

In recent years, chromatography has been gradually used in protein refolding from denatured state to biologically active conformation.

近年来层析技术的一个拓展是用于辅助蛋白质从变性态恢复到活性态，即所谓的复性，取得了令人瞩目的进展。

The synergy of surfactant and denaturing environment for protein refolding was also confirmed.

表面活性剂与变性环境对于蛋白质的折叠具有协同效应。

The protein purified with Ni-NTA column was denatured by 8 mol/L urea and dialyzed for refolding.

Ni-NTA柱纯化后的特异性蛋白经8 mol/L脲素变性，梯度透析使蛋白质复性。

Next, the oxidative refolding of denatured-reduced Lysozyme in presence of cetyltrimerthyl ammonium bromide and β-cyclodextrin were investigated.

接着研究了去污剂十六烷基三甲基溴化铵和β-环糊精的人工分子伴侣体系对变性-还原溶菌酶的氧化复性效果。

The chaperone activity of porcine kidney peptidyl-prolyl cis-trans isomerase was studied. The results showed that inactive or active PPIase was able to improve the refolding yields of creatine kinase , and inhibited aggregation. However, alkylated PPIase was not able to prevent CK aggregation.

研究猪肾脯氨酰顺反异构酶的伴侣功能时发现有活性和无活性PPIase能够提高肌酸激酶再折叠产量，抑制肌酸激酶聚沉，但是烷基化PPIase丧失防止肌酸激酶聚沉的能力。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新