查询词典 refolding

With the denaturation and protein refolding, we gained the recombinant PlyC. To determine its biological activity, we adopted turbidimetry and plate count method. Before and after lysin treatment, the cell morphology was studied by scanning electron microscopy.

用透析复性方法得到目的产物重组链球菌噬菌体裂解酶PlyC，以浊度法和平板计数法检测其体外抗菌效果，扫描电子显微镜观察裂解酶作用前后细菌细胞形态变化。

It seemed a pleasant excitement to her, this folding and refolding of silks and velvets, this gathering together of jewels and millinery.

把绸缎和丝绒衣服折叠又折叠，把珠宝和头饰都收集拢来，对爵士夫人说来，凡此仿佛是一种愉快的刺激。

The E. coli ClpQY is an ATP-dependent protease that consists of an ATPase large subunit and a peptidase small subunit. The function of ClpQY protease is as a chaperon in refolding of misfolded peptides and as a protease to catalyse the degradation of abnormal proteins as well.

大肠杆菌ClpQY是一个ATP依赖的蛋白酶，ClpQY蛋白酶包含了具ATPase活性的大蛋白分子和一个小分子的peptidase，ClpQY蛋白酶作为一伴随蛋白质扮演分解错误摺叠的蛋白质，也为一催化分解不正常的蛋白质的蛋白酶角色。

The inclusion bodies were solubilized in 50mM Tris-buffer at pH 12.5 and then were diluted to refold the denatured proteins. Refolding of D-hydantoinase by direct and fed-batch operations was investigated . Furthermore, the influence of the additives, CTAB and MnCl2, for soluble protein recovery was also studied.

实验中先以pH 12.5， 50mM Tris-bufferru将包涵体溶解，再藉由稀释法使其复性，并探讨直接稀释法与馈料稀释法对乙内醯尿酶复性的影响，另外探讨添加剂是否对可溶蛋白回收率有所帮助。

For the paramyxoviruses, the fusion protein catalyses this membrane merger and entry step, and it has been postulated that the F protein undergoes complex refolding during this process.

涉及这一过程的很多病毒蛋白是高度&专业&的。

The oxidative refolding of denatured-reduced lysozyme in the presence of cetyl trimethyl ammonium bromide and/or β-cyclodextrin was investigated.

研究了β-环糊精和表面活性剂十六烷基三甲基溴化铵作为复性液添加剂对促进变性-还原溶菌酶的氧化复性。

The effect of guanidine hydrochloride concentration on the oxidative refolding of denatured-reduced lysozyme of high concentration in the presence of artificial chaperone , that is, cetyl trimethyl ammonium bromide and β-cyclodextrin,was studied.

研究了表面活性剂十六烷基三甲基溴化铵加β-环糊精和盐酸胍对高浓度变性-还原溶菌酶氧化复性的影响，利用基于二态复性模型的表观动力学模型分析了溶菌酶的复性过程。

The purified TGF β1 monomer protein can form into active dimeric TGF β after refolding in vitro.

纯化的TGFβ1单体蛋白通过体外再折叠可以形成具有生物活性的TGFβ1双体分子。

A new eucaryotic expression system of recombinant PoIFN-γ was also constructed so that the denaturalization and refolding process is avoided. The target gene coding PoIFN-γ2 mature protein was subcloned into expression vector, transformed into Pichia pastoris, The His〓 Mut〓 phenotype transformants were screened, fermented in flasks and induced by 1％methanol. The expressed product in culture solution and sedimentation have antiviral activity on VSV and immunological activity proved by indirected immunofluorescence antibody and Dot blotting assay.

为了构建可对表达产物修饰、加工的真核表达系统，使表达产物具有自然干扰素的活性，无需变性、复性，我们将PoIFN-γ2成熟蛋白基因连接酵母整合表达载体，电转化毕赤酵母，筛选了His〓Mut〓表型转化子，摇瓶培养，1％甲醇诱导表达，培养上清和菌体裂解上清均有抗VSV病毒活性，经间接免疫荧光抗体检测和Dot blotting鉴定，重组酵母菌诱导表达产物具有免疫活性。

Under different initial total egg white lysozyme concentrations in urea solution, the refolding egg white lysozyme intermediates could be deduced to have a tendency to form a bimolecular intermediate aggregate, and this inference was further confirmed by their nonreducing SDS-PAGE and size exclusion chromatography.

当不同浓度蛋白溶菌酶在脲溶液中复性时，蛋白溶菌酶折叠中间体有形成二分子积聚体的趋势，这个推测得到了非还原电泳和体积排阻色谱结果的证实。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新