查询词典 refolding

Acetamide and thiouera had the parallel effect with GdnCl on the renaturation of lysozyme , both of them were able to prevent the aggregation of peptide, and at the high concentration they were able to restrain the refolding of peptide , but the structure of lysozyme under experiment condition could not be destroyed by them.

乙酰胺和硫脲则起着和盐酸胍近似的作用，能够抑制肽链间的聚集和肽链内的折叠，但是两者在本实验浓度内不能使溶菌酶变性。

All of them could facilitate the refolding of eMDH with lower concentration, especially for sucrose and betaine.

因此首先考察了三种渗透剂辅助eMDH包含体复性的情况。

The role of arginine to assist protein refolding was similar to that of acetone, both of them were able to combine with the denature lysozyme and improve the refolding yield.

结果表明，与自发复性收率(40%)相比，复性液中添加精氨酸、甘油、乙酰胺、丙酮、硫脲、海藻糖和甜菜碱均能有效提高变性溶菌酶的复性收率。

Although human leptin has been shown to dimerize during refolding procedure, the exact dimerization mechanism still remains unillustrated.

在前期研究中，已发现人瘦素在体外再折叠过程中会形成稳定的二聚体，但其二聚化机制尚不清楚。

Purpose: Study on the refolding of inclusion body of recombinant human granulocyte colony stimulating factor.

研究人粒细胞集落刺激因子包涵体的复性方法。

Refolding of reduced/denatured Lys was compared between using SEC and adsorption chromatography.

比较了还原变性Lys在SEC色谱柱上的复性，认为SEC是一种较好的色谱复性方法。

In this thesis, ribonuclease A was employed as a model protein to understand the formation of correct three-dimensional structure in the refolding of recombinant proteins in vitro. On this basis, the refolding mechanism in vitro was discussed.

本文以核糖核酸酶A为蛋白质体外重折叠的模型蛋白，对其体外重折叠过程进行了研究，探讨了这类目标蛋白复性过程的一些规律性的问题。

Sodium lauroyl sarcosine was used as a denaturant, a novel protein refolding method, which was proposed on the basis of one step-AMC and IEC, one step-AMC-IEC was used on the refolding of denatured/reduced lysozyme.

一步人工分子伴侣-离子交换色谱法辅助还原变性溶菌酶的复性：以月桂酰基肌氨酸钠(Sodium Lauroyl Sarcosine，Sarkosyl，SLS)作为蛋白变性剂，发展了一步人工分子伴侣-离子交换色谱法，对还原变性溶菌酶进行了复性研究。

Results of non-reducing sodium dodecyl sulphate polyacrylamide gel electrophoresis of the supernatant and aggregate precipitate formed in refolding process show that except being refolded to native egg white lysozymes, the reduced-denatured lysozymes can also form the aggregates with molecular weights being separately about 30.0 and 35.0 kD, while the reducing SDS-PAGE and the refolding results in the presence of sodium dodecyl sulphate show that these aggregates are formed chiefly through the misconnection of disulfide bonds between the reduced-denatured lysozymes, and the aggregate precipitates are formed through the non-covalent interactions between the aggregates with molecular weight being about 30.0 kD.

复性过程形成的上清和集聚体沉淀的非还原电泳结果表明，除了能复性成天然态的溶菌酶分子外，还原变性蛋白溶菌酶同时还能形成分子量分别约为30.0KD和35.0KD的蛋白溶菌酶分子集聚体；而它们的还原电泳和在SDS存在时的复性结果表明，这些集聚体主要是通过还原变性蛋白溶菌酶分子间的二硫键错配而形成，而集聚体沉淀则是通过分子量约为30.0KD的集聚体分子之间的非共价相互作用而形成。

The comparison of the refolding constants of the two proteins was made to get a better understanding of the two protein refolding processes.

为了对两种蛋白质复性的不同之处有更好的了解还对两者的复性动力学常数加以了比较。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失