查询词典 refolded

The recombinant protein GFP-SA was purified through the Ni-NTA affinity chromatography, and then refolded.

用IPTG诱导重组蛋白的表达，用镍金属螯合层析柱进行纯化。

Inclusion bodies of expressed proteins were dissolved in denaturants and purified by NTA-Ni gel and refolded under suitable conditions.

用变性剂溶解包涵体蛋白，经Ni-NTA琼脂糖纯化后在适宜的条件下进行重折叠复性。

According to structure analysis, the contents of α-helix and dimer structure in refolded eMDH were increased by the cooperation, and eMDH activity was enhanced.

对复性产物结构的初步分析发现，耦合作用可使eMDH分子的α-螺旋及二聚体含量明显增多，因此复性产物的活性也显著提高。

The expressed protein, His-EGFP-CRP, was purified with affinity chromatography method and refolded with gradient filtration.

目的：表达纯化重组的His-EGFP-CRP蛋白，通过毛细管电泳技术对该重组蛋白的生物活性进行评价。

A photograph of my father. Family wedding announcements. And a one-page letter, folded and refolded many times.

拉开那个暗隔间，我发现里面放了些文件：一张父亲的照片，家庭成员的结婚公告，还有一封展读多次、仅一页纸内容的信。

He demonstrated the art. He folded, unfolded and refolded.

他向人们示范着这门艺术--折好，拆开，再折好，并向人们解释复杂的细节以及必须把折皱处弄得平滑光洁、有棱有角。

The results showed that the purified recombinant protein under denaturing conditions was refolded in Ni-NTA column by using refolding buffer.

实验结果表明，在变性条件下纯化的样品在柱上不经洗脱，直接用复性液洗涤6h，可以使EKLC在得到提纯的同时实现复性，得到了电泳纯度为96％的具有生物活性的EKLC，最高活性为712U。

The UV absorption peak of Hb-acyclovir complex disappeared, and the tight structure of Hb aroused by acyclovir was refolded.

Hb-阿昔洛韦的复合物的吸收峰消失，由阿昔洛韦引起的Hb的紧密结构得到重新折叠。

Moreover, the product refolded from inclusion bodies of E. coli shows the predominance in homogeneity and the lower cost.

说明E。 coli表达体系可生产质量均一、高生物学活性的rhNGF-β，且具有明显成本优势。

Therefore the denatured protein could pass along the gradient and refolded to native conformation as urea was gradually removed and a linear removal of urea was realized in this manner.

在与常用复性方法的比较中，这种方法在模型蛋白质溶菌酶的复性实验中初步显示出了一定的优越性。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失