查询词典 refolded

Opening the billfold, he carefully removed two worn pieces of notebook paper that had obviously been folded and refolded many times.

丧礼之后，马克的老同学大部分都前往查克的农舍用午餐。马克的父母也在那儿，显然是在等我。

Opening the billfold, he carefully removed two worn pieces of notebook paper that had obviously been taped, folded and refolded many times.

他打开皮夹，小心翼翼地拿出两张用旧的笔记本纸，它们明显地被用绳子捆扎过，并且反复折叠了多次。

It was actually Tertia who found the notes, some sheets of foolscap paper folded and refolded and crammed into a little crack where the wainscoting had become separated from the wall.

实际上发现笔记的是特西娅，几页打印纸被折了又折然后塞进了一个从墙上裂开壁板缝里。

The three-dimensional fold of the Gamma crystallin has been solved and the protein can be refolded in vitro .

晶状蛋白可在试管内再折叠且其三维立体结构已经被解出。

The three-dimensional fold of the Gamma crystallin has been solved and the protein can be refolded in vitro .

Gamma晶状蛋白可在试管内再摺叠且其三维立体结构已经被解出。

After inclusion bodies was denaturalized and refolded in vitro, significant assembly of monomers had occurred, and the recombinant protein represented assembled pentamers.

Western blotting分析结果显示CTB-INSB可分别被霍乱毒素的抗体和胰岛素的抗体识别，表明该蛋白具有霍乱毒素B亚单位与胰岛素的双重抗原性。

The unfolded protein correctly refolded to the native conformation through the column, and was partly purified during the elution process.

其原理是将层析技术应用于蛋白质复性和纯化，使变性蛋白质在层析柱上折叠为正确的空间构象，在洗脱的同时实现部分纯化。

METHODS: The rhBMP-2,expressed in Escherichia coli, was washed by Triton X- 100 and further purified by DEAE chromatography.The inclusion bodies were resolved in 8 mol/L urea, and were refolded and dimerized in the redox systems (reduced and oxidized glutathione). Finally, a one - step purification procedure based on the heparin affinity chromatography was implemented. The biological activity of purified rhBMP - 2 were tested by induction of the alkaline phosphatase activity in C2C12 cells.

在大肠杆菌中通过温度诱导表达重组人骨形成蛋白-2经过Triton X-100清洗之后，又通过DEAE离子交换层析纯化包涵体，包涵体在8 mol/L尿素变性溶解，在氧化-还原(还原型和氧化型谷胱甘肽)复性系统中，通过简单的稀释复性，通过肝素亲和层析一步纯化法纯化重组人骨形成蛋白-2，最后通过诱导C2C12细胞产生碱性磷酸酶检测重组人骨形成蛋白-2活性。

The rhBMP - 2 was refolded and dimerized in the redox systems (reduced and oxidized glutathione) and a one - step purification procedure based on the heparin affinity chromatography was implemented to isolate the rhBMP - 2 dimers and monomers.

亲和层析纯化后，本实验得到重组人骨形成蛋白-2的生物学活性比商业化的重组人骨形成蛋白-2更高。

Results of non-reducing sodium dodecyl sulphate polyacrylamide gel electrophoresis of the supernatant and aggregate precipitate formed in refolding process show that except being refolded to native egg white lysozymes, the reduced-denatured lysozymes can also form the aggregates with molecular weights being separately about 30.0 and 35.0 kD, while the reducing SDS-PAGE and the refolding results in the presence of sodium dodecyl sulphate show that these aggregates are formed chiefly through the misconnection of disulfide bonds between the reduced-denatured lysozymes, and the aggregate precipitates are formed through the non-covalent interactions between the aggregates with molecular weight being about 30.0 kD.

复性过程形成的上清和集聚体沉淀的非还原电泳结果表明，除了能复性成天然态的溶菌酶分子外，还原变性蛋白溶菌酶同时还能形成分子量分别约为30.0KD和35.0KD的蛋白溶菌酶分子集聚体；而它们的还原电泳和在SDS存在时的复性结果表明，这些集聚体主要是通过还原变性蛋白溶菌酶分子间的二硫键错配而形成，而集聚体沉淀则是通过分子量约为30.0KD的集聚体分子之间的非共价相互作用而形成。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失