查询词典 refold

Heat shock proteins serve as molecular chaperons,binding to misfolded proteins,assisting to refold misfolded proteins and eliminate irreversibly damaged proteins.

热休克蛋白(heat shock protein，HSP)作为分子伴侣，与错误折叠的蛋白质结合，促进其正确折叠或清除。

The inclusion bodies were solubilized in 50mM Tris-buffer at pH 12.5 and then were diluted to refold the denatured proteins. Refolding of D-hydantoinase by direct and fed-batch operations was investigated . Furthermore, the influence of the additives, CTAB and MnCl2, for soluble protein recovery was also studied.

实验中先以pH 12.5， 50mM Tris-bufferru将包涵体溶解，再藉由稀释法使其复性，并探讨直接稀释法与馈料稀释法对乙内醯尿酶复性的影响，另外探讨添加剂是否对可溶蛋白回收率有所帮助。

Methods Split the recombinant E. coli for expression of rhOP-1 by ultrasomication in ice bath and extract the inclusion body. Dissolve the inclusion body with 8mol/L urea, purify by SP-Sepharose chromatography, refold by gradient dialysis.

将表达rhOP-1的大肠杆菌菌体在冰浴下超声裂解，分离提取包涵体，用8mol/L尿素溶解，纯化后进行梯度透析复性。

HCV NS3C/NS4 protein fused with E. coli thioredoxin was expressed insolubly although thioredoxin is a highly soluble fusion partner. NS5B-dc21 protein was soluble, which is a truncated version of NS5B, while NS5AB protein was insoluble, which needed resolubilization and refold procedures.

HCV NS3C/NS4蛋白是以硫氧还蛋白融合表达的方式获得的，尽管硫氧还蛋白有很强的助溶作用，该蛋白依然不可溶形式存在；NS5B—dc21蛋白通过C末端疏水序列的删除，获得了可溶表达；NS5AB蛋白以不可溶形式表达，需要复性。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新