查询词典 reference mark

Three RNAi target sites (named as Abil1, Abil2, Abil3) targeting the E3B1 (NCBI: NM-024397), an identified target sites and positive control GAPDH-A were selected. The pGenesil-1 eukaryotic expression vectors with the neoR mark and GFP green fluorescent mark were selected to construct E3B1 RNAi plasmid. The effect of RNAi targeting various sites on E3B1 genes expression was evaluated by testing the transfection efficiency with fluorescence microscope and western blot. The most effective siRNA in inhibiting E3B1 genes were screened. The most effective siRNA in inhibiting E3B1 genes screened and used to transfect the neuronal cells. After that, the inhibitor of axon growth was added and the axon growth was observed. Result: The siRNA expressing plasmids targeting the E3B1 were constructed successfully.

选择针对E3B1(NCBI： NM-024397)RNAi的3个靶位点Abil1、Abil2、Abil3和1个不针对任何mRNA的RNAi靶位点以及甘油醛－3-磷酸脱氢酶，用带有neoR选择标志和GFP绿色荧光标志的真核表达载体pGenesil-1构建E3B1 RNAi质粒，分别转染培养的神经元，在荧光显微镜下观测转染率，经G418筛选得到单一的转染细胞，并用Western blot法检测各转染组神经元E3B1蛋白的表达情况，选出具有最佳抑制效应的siRNA转染神经元，加入轴突生长抑制物，观测轴突生长情况。

This mark is also called Local Fiducial Mark.

该标记也称为"局部基准标记"。

An observer should not attend the flagstick , stand at or mark the position of the hole , or lift the ball or mark its position.

观察员不应照看旗杆、立于或标示球洞所处位置、或者将球拿起或对球位作标记。

A referee should not attend the flagstick , stand at or mark the position of the hole , or lift the ball or mark its position.

裁判员不应照看旗杆、立于或标示球洞所处位置、或者将球拿起或对球位作标记。

A gun crew, that is to be called upon in time of danger to hit a moving mark at the distance of several miles, a mark invariably out of sight, must have had considerable practice in time of peace to be able to hit anything in the excitement of battle.

一个炮手，是在危机关头被召唤来对数里开外的移动目标进行射击的，这个目标当然是目视观察不到的，要做到在战斗的激动之下在任何情况下都命中目标，那就需要在和平时期大量的训练。

To correct this, move forward a foot or eighteen inches and try again. When you find the distance from the target at which your knives stick consistently when thrown one-half turn in a way that's comfortable for you, mark the spot: drive a stake in the ground, build a cairn, hire someone to stand there with a flag, but mark that spot, and always practice half-turn throws from there.

当发现在某一距离上你能经常命中靶子，并且使用半周旋转投掷法时感觉舒适，则在此距离上作出标志：钉一个木桩、放一块石头、插上旗子等，并且始终在此距离进行半周投掷。

President Bush had just been re-elected and was preparing his triumphal second inauguration that was to mark the high-water mark for neoconservatism.

总统布什刚刚连任，并准备他的第二任获胜演说，继续为新保守主义鼓吹其功绩。

The hot line that profiled bar factory is Lai steel Inc. produces one of units, have small-sized, medium-sized and large 3 section product line, basically produce product of profiled bar of etc of light-duty series, medium-sized series and profiled bar of large series H, main body equipment is advanced, year productivity 2.4 million tons of above. 2004, h profiled bar passed product certification of Chinese ship's classification society, the mark is worn the pass that profiled bar of Lai steel H had hull to use steel and marine oil platform to use steel. 2006, passed day bid and Europe mark attestation respectively, hold one's head high push forward Japan and Europe.

梗概： 型钢厂是莱钢股份有限公司的热线生产单位之一，拥有小型、中型和大型三条型钢生产线，主要生产轻型系列、中型系列和大型系列H型钢及其他型钢产品，主体设备先进，年生产能力240万吨以上。2004年，H型钢通过了中国船级社产品认证，标志着莱钢H型钢拥有了船体用钢和海洋石油平台用钢的通行证。2006年，分别通过了日标和欧标认证，昂首挺进日本和欧洲。

It had wooden puppets to beat a drum to announce a quarter, to ring a hand-bell to mark an hour, hang out a plate to tell time every other hour and to beat a large bell to mark the watches in the night.

水运仪象台的计时机械部分可以按时刻使木偶出来击鼓报刻，摇铃报时，示牌报告子、丑、寅、卯十二个时辰，击钲报更等。

Ms. Roper said lawmakers need to understand 'that the alternative to mark-to-market accounting is mark-to-myth' and could give banks and other financial companies the freedom to value assets at inflated amounts.

罗泊尔称，议员们应该清楚，按市值计价会计准则的替代品会是"按幻想计价"，这会让银行和其它金融机构肆意虚定高价。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。