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The human cartilages are composed of chondrocyte and extracellular matrix , the form of chondrocytes are hypertrophy and the quantity are less; the ECM of cartilage are compised of type Ⅱ collagen and proteoglycan. Articular cartilages are all hyaline with little fibers. Trauma and arthritis are the main cause of cartilage injury, the ommilayer injury ofcartilage can be recovered by marrow, but because of without stimulation mechanism, the new tissues are merely fibrocartilages, they can not be coincide with hyaline cartilage in menchanics; the purely damage of articular cartilage can not stimulate chondrocyte to regenerate because of without blood circulation, thus, the plerosis of articular catilage can not depend on the proliferation of local chondrocyte. Ever since, people tried their best to find a way to reconstruct articular cartilage.

中文题名人骨髓基质干细胞成软骨诱导及多孔复合材料作为细胞载体的体外实验研究副题名外文题名 Cartilage induction of human mesenchymal stem cells and experiment on compound porous materials as cells' scaffold in vitro 论文作者刘晓岚导师周江南学科专业外科学研究领域\研究方向学位级别博士学位授予单位中南大学学位授予日期2003 论文页码总数68页关键词骨组织工程软骨细胞骨髓基质干细胞壳聚糖高分子外消旋聚乳酸馆藏号BSLW /2003 /R68 /10 造成人体关节软骨损伤的原因主要为创伤和关节炎，关节软骨全层损伤可由于骨髓中间充质干细胞的高速增殖修复，但这种修复由于缺乏相应的刺激机制，只能形成纤维软骨，而不能形成符合关节生理、力学要求的透明软骨；单纯软骨部分损伤软骨组织内无血管，软骨细胞迁移迟缓，无法使损伤区域软骨细胞再生，因此，关节炎及关节创伤后的软骨修复不能依赖于软骨细胞的增殖和迁移。

Here, we report a case of acute Q fever complicated with mild jaundice and pleuritis. He did not respond well to doxycycline or levofloxacin initially, but recovered subsequently with adjuvant steroid therapy.

在此报告一例急性Q热合并黄疸及肋膜炎的个案，病患住院接受doxycycline及levofloxacin治疗但反应不佳，合并类固醇治疗后痊愈。

Here, we report a case of acute Q fever complicated with mild jaundice and pleuritis. He did not respond well to doxycycline or levofloxacin initially, but recovered subsequently with adjuvant steroid therapy.

在此报告一例急性Q热合併黄疸及肋膜炎的个案，病患住院接受doxycycline及levofloxacin治疗但反应不佳，合併类固醇治疗后痊癒。

The preserved frozen thalli of Porphyra yezoensis were recovered in different light and temperature conditions.

摘 要 对冷藏的条斑紫菜叶状体在不同光照和温度条件下进行复苏实验。

The patient received early antiviral therapy and intensive rehabilitation. Two months later, her shoulder muscle strength improved although it hadn't recovered to her premorbid status.

发病二个月后追踪病人的复原情形，她的右肩带肌力虽未恢复到发病前的状态，但几乎可以从事大部分的功能性活动。

Expression rate induced by thrombin was 50.88% and PAC?1 re?expression was 54.55%. It is concluded that the ability of recovered lyophilized platelets added with reversible activation?inhibitors, DMSO and trehalose to aggregate and coagulate has showed no significant difference as compared with FPRP. The reversible activation?inhibitors can decrease CD62p expression of lyophilized platelets, and may enhance their survival ability and prolongate survival time. Therefore the efficiency of lyophilizing platelets can be improved based on this freeze?drying procedure.

添加血小板可逆性激活抑制剂，海藻糖和DMSO后的冻干血小板，其聚集活性和促凝血功能与新鲜血小板无明显差异，血小板可逆性激活抑制剂降低了冻干血小板的CD62p表达，增强了冻干血小板的生存能力，因而延长了其生存时间，因此可以该冻干方法为基础进一步提高冻干保存血小板的效率。

CD62p reexpression rate induced by thrombin was 50.88% and PAC1 reexpression was 54.55%. It is concluded that the ability of recovered lyophilized platelets added with reversible activationinhibitors, DMSO and trehalose to aggregate and coagulate has showed no significant difference as compared with FPRP. The reversible activationinhibitors can decrease CD62p expression of lyophilized platelets, and may enhance their survival ability and prolongate survival time. Therefore the efficiency of lyophilizing platelets can be improved based on this freezedrying procedure.

添加血小板可逆性激活抑制剂，海藻糖和DMSO后的冻干血小板，其聚集活性和促凝血功能与新鲜血小板无明显差异，血小板可逆性激活抑制剂降低了冻干血小板的CD62p表达，增强了冻干血小板的生存能力，因而延长了其生存时间，因此可以该冻干方法为基础进一步提高冻干保存血小板的效率。

MATERIALS AND METHODS: Arg to Ser mutation was introduced into the 249 position of the p53 gene by knock-in method. These ES cells with this mutation were selected according to the homologues-recombination with PCR and Southern blot. The positive ES cells without a selection marker were injected into blastocysts recovered from Hprt(superscript -/-) mice, which were derived from Hprt-deficient ES cells. The injected blastocysts then were implanted into pseudopregnant females.

材料与方法：利用基因打靶技术在小鼠胚胎干细胞p53基因249编码子中引入点突变，使编码子249由精氨酸变成丝氨酸，然后将含突变的ES细胞显微注射到Hprt小鼠囊胚中，将注射过的囊胚植入假孕的雌性小鼠子宫，到第14d取小鼠胚胎纤维母细胞，用含HAT的培养液筛选出从ES细胞分化而成的鼠EF细胞，经测序证实细胞含有由249Arg到Ser的突变。

There were no statistically differences between the 8th~ 14thday, the 15th - 21th day post-BC and the control group. During the rt~24tkday post-BC , pycnosis degeneration or necrosis neurons of the locus cerebral cortex, dorsal hippocampus, dentate fornix were significantly increased, then decreased gradually, but recovered to normal by the 24thday after BC, and especially in parietal cortex and piriform cortex the necrosis neurons were significantly increased than temporal cortex , and there were no statistically difference berween the left and the right side. Pycnosis degeneration or necrosis neurons in the brainstem reticular formation were markedly increased in the 4thday after BC, and there were no statistically difference among the other groups and the control group.

与对照组相比，BC后在大脑皮质、背侧海马和齿状回部位，固缩变性和不完全坏死细胞数先显著增加(P＜0.05)，然后逐渐减少，至24d基本恢复正常；顶叶、梨状皮质比颞叶皮质变性坏死细胞多，差异有高度显著性(P＜0.01)；大脑左侧比右侧变性坏死细胞稍少但差异无显著性；在BC后4d，脑桥核、斜方体核平面的脑干网状结构中固缩变性和不完全坏死细胞数明显增多(P＜0.05)，其它组间无显著性差异。

Pyke to find le Sam, Le some of the money recovered, but this is right greed の de couple has spent 750,000 U.S. dollars of which Le, Pyke want them to refund the balance within 5 days. March 26 until the U.S. release の de film, New Year's surprise, Le pirated DVD, will look at the Le.

Pyke寻找到勒Sam，追回勒部分款项，但是这对贪婪のde夫妇已经花去勒其中75万美元，Pyke想要他们在5日内退还余款。3月26日才美国上映のde片子，春节前竟然有勒盗版DVD，就先看勒。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。