查询词典 recombined

With the method of reverse transcription-polymerase chain reaction and nest PCR, P80 gene of CSFV is cloned from the cells infected by the strain Shimen of csfv, and connected with eukaryotic expression vector pEGFP-C1. Then recombined eukaryotic expression plasmid pEGFP-P80 of P80 gene is achieved successfully. It builds the basis for expressing and gaining protein p80 in mammiferous cells.

利用反转录PCR和套式PCR技术，从猪瘟病毒Shimen株细胞毒中克隆出P80 基因，并进一步把P80 基因连接在真核表达载体pEGFP-C1 上，成功地构建出P80 基因重组真核表达质粒(pEGFP-P80)，为在哺乳动物细胞中表达并纯化出p80 蛋白奠定了基础。

Then pMD-P80 is digested by the enzymes Xho I and Apa I, separated and linked to the linear plasmid pEGPF-C1 after digested by Xho I and Apa I. So the recombined expression plasmid pGFP-P80 is achieved. Then it is sequenced, PCR and digested by the restriction enzymes Xho I and Apa I. The results give that pGFP-P80 is achieved successfully and the insert sites, direction and reading frame are all right. It builds the basis for expressing, purifying, gaining p80 protein from mammiferous cells.

将pMD-P80 分别经Xho I 和Apa I 双酶切和回收，然后与经过Xho I 和Apa I 酶解的真核表达载体pEGPF-C1 连接、转化，获得重组质粒，经PCR，Xho I 和Apa I 限制性酶切和序列测定，鉴定为真核表达质粒pEGFP-P80，并且目的基因的插入位置、方向和读码框完全正确，为在哺乳动物细胞中表达并分离纯化p80 蛋白奠定了基础。

In them Feanor has recombined and made whole the refracted gold and silver light of the Trees.

这种相似是故意为之的。

Three crosses between NG94-156 and three varieties were made, and F2 segregative population and two recombined inbred line populations(F7:8) were obtained.

曲茎遗传材料NG94-156与遗传背景不同的3个正常茎品种杂交，获得1个F2群体和2个重组自交家系(F7：8)。

The translationally controlled tumor proteingene of Plasmodium falciparum recombined with the pQE-30 vector was transformed into the E.coli M15 and induced with IPTG to express TCTP.

翻译控制肿瘤蛋白最初在小鼠肿瘤细胞中被发现，是一个受翻译过程所阻遏并与肿瘤生长相关的蛋白质，随后在酵母及植物和除肾细胞外的各种动物组织正常细胞中被发现(Sanchezet al。，1997)。

Orientalis in red blood cells from an infected calf was isolated. cDNA was synthesized by reverse transcriptase, amplified by PCR and ligated into λTriplEx2 vector. The recombined vectors were packaged and the unamplified cDNA library was constructed.

用东方巴贝虫感染牛，提纯红细胞内虫体的总RNA，反转录合成cDNA， PCR扩增后将其连接于噬菌体载体（λTriplEx2），通过体外包装，建成东方巴贝虫cDNA文库，并进行扩增。

With more and more Multinational Enterprises come into the medical equipments market in China, the market structure was recombined gradually.

随着越来越多的跨国企业进入中国医疗器材市场，市场的格局不断地重组和动荡。

Part of one gene could be recombined with part of other gene to give one normal gene.

一个基因的一部分和另一个基因的一部分能重组而产生一个正常基因。

The same sounds can be recombined to mean something else.

相同的语音能够重组来表达其他的事物。

Second, water loss is reportedly reduced by 99% because hydrogen and oxygen are recombined within the battery.

第二，水的损失，据报是减少了99 ％，因为氢气和氧气是重组内部的电池。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。