查询词典 recombination

Polymer substrates with dormant groups on the surface were prepared through the recombination of ITX pinacol radicals and surface radicals generated by the abstract hydrogen reaction.The influence of light intensity,irradiation time,concentration of ITX solution and polymer substrates to the dormant group density was investigated respectively.

论文取得的主要结果如下：1、利用夺氢型光引发剂异丙基硫杂蒽酮的夺氢-偶合光化学反应，将ITX嚬哪醇自由基固定到基材表面，制得了表面偶合有休眠基的聚合物基材。

Pombe. An active recombination of pREP3X-HA-mid2 was identified from 41 single clones of relevant transformed S.

在41个酵母转化单菌落中，筛选到一个有表达活性的pREP3X-HA-mid2重组子。

An active recombination of pREP3X-HA-spm1 was identified from 35 single clones of relevant transformed S. pombe.

在35个酵母转化单菌落中，筛选到一个有表达活性的pREP3X-HA-spm1重组子。

The main methods used, or under investigation, for production of rumen gene engineering bacteria are:gene deletion technique, gene recombination technique and the application of promotor.

正在应用的或处于研究阶段的构建瘤胃基因工程菌的方式主要有基因缺失技术、基因复制性重组技术和启动子的应用技术。

MATERIALS AND METHODS: Arg to Ser mutation was introduced into the 249 position of the p53 gene by knock-in method. These ES cells with this mutation were selected according to the homologues-recombination with PCR and Southern blot. The positive ES cells without a selection marker were injected into blastocysts recovered from Hprt(superscript -/-) mice, which were derived from Hprt-deficient ES cells. The injected blastocysts then were implanted into pseudopregnant females.

材料与方法：利用基因打靶技术在小鼠胚胎干细胞p53基因249编码子中引入点突变，使编码子249由精氨酸变成丝氨酸，然后将含突变的ES细胞显微注射到Hprt小鼠囊胚中，将注射过的囊胚植入假孕的雌性小鼠子宫，到第14d取小鼠胚胎纤维母细胞，用含HAT的培养液筛选出从ES细胞分化而成的鼠EF细胞，经测序证实细胞含有由249Arg到Ser的突变。

Chlamydophilophila psittaci MOMP ; recombination ; genetic ; adenoviridae ; neutralization tests ; chicks immunity

衣原体；鹦鹉MOMP ；重组；遗传；腺病毒科；中和试验；雏鸡免疫

Chlamydophilophila psittaci MOMP; recombination, genetic; adenoviridae; neutralization tests; chicks immunity

目前该病被称为&鹦鹉病&或&鹦鹉热&，该病最早被认为与鹦鹉以及与这些鹦鹉接触的人相关联。

The recombination vector was digested by Pac I enzyme and transfected into 293A cell by Lipofectamine method to obtain recomb...

该载体经PacI酶切，脂质体法转染人胚肾293A细胞，获得重组腺病毒载体pAD-E7.pAD-E7转染人HaCaT细胞，激光共聚焦显微镜分析E7蛋白表达情况。

The somatic hybrids usually showed specific bands and combined all the bands of both parents. In combinations, some new bands were detected, and occasionally specific bands of parents were lost, which indicated that chromosome recombination and exchange happened after fusion. Additionally, in some combinations, specific bands from leaf parent were detected, which revealed the plants were of putative hybrid origin, because leaf protoplast cannot redifferentiate by both single culture and co-culture.

体细胞杂种一般表现为具有双亲的特征带，且综合双亲的所有带，但在部分杂种中检测到了双亲都没有的新带，也发现有丢失亲本的特征带或共有带的现象，说明融合后染色体发生了重组和交换；有的引物只检测到叶肉亲本的特征带，根据柑橘叶肉细胞无论单独培养还是共培养均不能再生的事实，可推测其为体细胞杂种或二倍体叶肉亲本型胞质杂种。

It points out it is the conversion of property system that is the core and background of modern hospital management innovation, investigates the innovation of organizing-strucrure ,devises the recombination and reforger of hospital management by describing the working flow chart and reformer table and sets up a mode of probability.

4探讨了现代医院管理制度创新的对策，界定了现代医院管理制度创新的内涵，分析了现代医院制度的现状与问题，论述了加入世贸组织对医院管理制度创新的要求，提出了现代医院管理制度创新的核心和基础是医院产权制度的转变，研究了医院管理组织结构创新，设计了医院管理流程重组与再造，描绘了工作流程图和过程重建图，并建立了概率模型，通过二个案例分析提出了优化方案。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。