查询词典 recombinants

The recombinants with different multiple copies of amyL integrated in the chromosome were identified by real-time PCR and evaluated by shake-flask fermentation.

对转化子的amyL拷贝数及其BLA发酵水平分别用荧光实时定量PCR及摇瓶发酵试验进行评价与鉴定。

It was shown,based on the study,that cellobiose utility capability of the recombinants was increased,glycerol productivity of that was decreased,and the bglⅡ gene expression was stable in t.

结果表明，整合子利用纤维二糖的能力得到提高，产甘油能力下降，外源基因表达稳定；引入外源基因后，其对酵母增殖能力没有大的影响，仅形态发生变化；在以微晶纤维素为原料，结合纤维素酶发酵时，与亲代工业酿酒酵母相比，发酵液乙醇浓度最高增加69%。

Results: The recombinants with 4,8,16 and 32 copies of gG 112-127 were obtained.

结果：获得了4×、8×、16×、32×重复串联的阳性重组子。

A further development of these techniques makes it possible to obtain homologous recombinants.

这些技术的进一步发展，使获得同源的重组体成为可能。

Objective To study the relationship between DNA fragments in ENV gene of SIVmac and viral replicating ability. Methods Reciprocal recombinants were constructed by replacing the clonable DNA fragments of ENV region of the nonpathogenic SIVmac142 clone with the pathogenic SIVmac 239 clone.

猴免疫缺陷病毒(simian immunodeficiency viruses SIV)是一组在致病性和毒力方面具有多样性的灵长类动物病毒，其中，SIVmac239克隆感染的猴表现为与人类AIDS相似的疾病，称之为致病株，而SIVmac142克隆仅引起猴发生病毒血症，不发生临床综合征，称之为非致病株〔1〕。

To find out the difference between Mut+ and Muts recombinants we compared their expression of pokeweed antiviral protein in the same conditions.

在相同的培养条件下比较了Mut~+和Mut~s重组菌株表达PAP的异同。

Methods The HPV16E7 gene was cut to three parts, amplified by PCR, and connected individually with BPVL1 on plasmid PUC. With PVL1393 as a transfect vector, the BPVL1/HPV16E7 recombinants were transfected to baculovirus which subsequently expressed the chimeric BPVL1/HPV16E7 protein in the SF－9 cells. The recombinant proteins were then purified by centrifuge, sonication, sucrose and CsCl ultracentrifuge, and were identified by SDS-PAGE, Western blot, ECL and TEM.

HPV16E7基因分3段经PCR扩增后分别克隆入连有BPVL1的质粒PUC形成BPVL1-HPV16E7；以质粒PVL1393为载体将BPVL1-HPV16E7基因转染杆状病毒并在昆虫细胞中进行表达；用超声粉碎和蔗糖超离、氯化铯梯度离心等方法纯化以及免疫印迹、ECL、透射电镜等方法鉴定表达产物。

The glutenin patterns of multispikelet wheat line 10 -A and its recombinants were analyzed by using sodium dodeeyl sulphate-polyacrylamide gel electrophoric.

应用十二烷基硫酸钠聚丙烯酰胺凝胶电泳分析了多小穗小麦品系10-A以及来源于三交组合10-A／88-1643／川育12号的56个稳定品系的谷蛋白。

The protoplast fusion between hyperparasitic Streptomyces strain F46 and Actinomyces strain SC11 was studied in order to improve the effect of wild actinomyces strains to control plant diseases, solve the problems in application of the biocontrol strains, explore the method of protoplast fusion and get the recombinants with some excellent characteristics of both parents.

为了提高野生生防放线菌株的防病效果，克服以往生防菌应用方面存在的诸多弊端，探索简单易行的人工改良生防放线菌的途径，我们选用重寄生链霉菌F46和放线菌SC11作为亲本，进行了双亲原生质体融合研究，以期得到整合双亲多种优良性状的工程菌株。

Transcriptional activity of ACL recombinants normalized by Renilla was significant difference with pGL3-Basic expect for construct -27 and -15 (P＜0.01). Construct -919 contains highest activity. 3 times reduction of transcriptional activity from-919 to -679bp indicated that negative regulation factors located probably in this region. The activity started on construct -73 suggested the basal promoter activity was located within the -73 to +77bp region; Transcriptional activity of IDHβrecombinants was not significantly different between the recombinant -58 and pGL3-Basic. The activity started on construct -82, decreasing with the length of the fragment up to -164 in despite of a bit of fluctuation, and kept increasing from construct -164 up to -279. Thus, the basal promoter activity was located within the -82 to +16bp region, whereas the upstream 197bp conferred maximal transcriptional activity.

采用5'端缺失策略，结合启动子预测结果，分别构建了8个ACL基因和19个IDH3β基因启动子区重组子，将其转染猪PK15细胞系，并利用荧光素酶双报告基因系统检测了它们的活性，结果表明：在所构建的猪ACL基因5侧翼序列的8个重组子中，除pGL-ACL27和pGL-ACL15外，其它重组子的荧光素酶活性与阴性对照均达到极显著水平(P＜0.01)，表明它们均具有启动子活性，pGL-ACL919活性最强，到pGL-ACL679活性下降了将近3倍，说明从-919bp到-679bp区域可能存在负的调控位点，从pGL-ACL621到pGL-ACL73活性有小幅度下降，到pGL-ACL27活性降到与阴性对照水平无显著差异，表明维持该启动子的基本活性区域位于-73bp到+77bp之间；IDH3β基因启动子活性开始于pGL-IB82，然后虽然有些波动，但是活性一直下降直到-164bp处，之后活性又开始升高，直到-279bp处活性达到最高，这些表明维持该启动子的基本活性区域位于-82bp到+16bp之间，从-82到-279之间的179bp区域具有最高的启动子活性。

Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势，并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。