查询词典 recombinant

The pcDNA〓-apoE〓 plasmid was transfected to SK-N-SH Neuroblastoma cell by liposome. It could be screened by G〓. The immunohistochemical assay was shown that neuroblastoma cell transfected by pcDNA〓-apoE〓 expressed the recombinant apoE protein. The intracellular expressed recombinant protein could inhibit the death of neuroblastoma cell induced by beta amyloidal peptides 25-35 fragment.

用pcDNA〓—apoE〓真核表达载体与脂质体共转染神经成纤维胶质瘤细胞，用G〓选择压力筛选，细胞免疫荧光检测表明，pcDNA〓—apoE〓转染的神经成纤维胶质瘤细胞表达了apoE〓重组蛋白，这种内源性的apoE〓重组蛋白对25μM Aβ25—35多肽诱导的神经成纤维胶质瘤细胞的死亡具有拮抗作用。

This is the first report of successful expression of chymosin in Pichia pastoris. The recombinant Pichia pastoris strain obtained in this study could be further used to produce recombinant chymosin for cheese making.

本研究首次应用毕赤酵母表达牛凝乳酶，在培养基中获得分泌表达的重组凝乳酶，为干酪工业提供了新型及优良的凝乳酶来源。

Objective To construct prokaryotic recombinant plasmids of transcriptional co-activator gene of Clonorchis sinen sis, express and purify the recombinant protein and analyze its biological function.

摘要目的构建华支睾吸虫成虫转录辅激活因子(transcriptionalcoactivator ，TC)基因原核重组质粒，进行原核表达、鉴定及生物功能分析。

Lmt.The characteristics and novelty in this dissertation was obvious in the following aspects: cofermentation of glucose and xylose to ethanol by recombinant yeast S. cerevisiae ZU-10, ethanol production from hydrolysates of cellulose and hemicellulose in corn stover by recombinant yeast S. cerevisiae ZU-10, and integration of key technologies in ethanol production from lignocellulosic biomass. These results were meaningful not only in academic research, also in utilization of renewable lignocellulosic biomass, decreasing the production cost of fuel ethanol , and alleviating the world grain crisis.

本文在基因重组酵母对葡萄糖和木糖的共发酵、利用基因重组酵母厌氧条件下发酵玉米秸秆纤维素和半纤维素水解液生产乙醇、以及利用木质纤维原料生产乙醇的关键技术集成等方面的研究具有明显的特色与创新，相关研究结果不仅有一定的学术价值，而且在促进可再生木质纤维素资源的转化利用、缓解人类的粮食危机、降低燃料乙醇的生产成本等方面具有重要的现实意义。

Recombinant adenovirus was identified by polymerase chain reColon cancer cell line SW480 was infected with recombinant adenovirus Ad-p27mt, and expression of p27 protein was detected by Western blot.of expressed p27 protein after Ad-p27mt transfected colon cancer cell.novirus framework plasmid pAdeasy-1 with pShuttle-CMV-hp27mt, 30％combinant adenovirus DNA contained the target gene.

Ad-p27mt的聚合酶链反应检测Ad-p27mt转染大肠癌细胞后的p27mt的表达。结果：①用含pShuttle-CMV-hp27mt转化含pAdeasy-1的超感受态BJ5183后，可获得约30％的阳性重组质粒。②聚合酶链反应检测表明重组腺病毒DNA中含有目的基因。重组腺病毒滴度为7.95×1015pfu/L。

METHODS: To construct recombinant expression plasmid subtype H1 of SIV HA gene of A/Swine/Guangdong/LM/2004(H1N1).BALB/c mice of 6-8 weeks old were immunized endemically with the recombinant plasmid.The splenocytes from the immunized mice were fused with Sp2/0 myeloma cells after the last immunization.

构建 H1 亚型猪流感病毒A/Swine/Guangdong/LM/2004(H1N1)HA基因表达质粒，基因免疫6～8周龄雌性BALB/c小鼠，加强免疫后取其脾细胞与骨髓瘤细胞 Sp2/0进行融合。

The adhesion test showed that small intestinal epithelium cells of 30-35-day-old postweaning piglets with both genotype M307~ and M307~ could adhere to the standard E.coli strain express F18ab fimbriae, the recombinant E.coli express F18ac rE.coli1534 and the recombinant pnirBMisL-fedF E.coli displaying FedF subunit on the surface of E.coli, but small intestinal epithelium cells with genotype M307~ and 3-day-old piglet could not adhere to the three kinds of bacteria descried above, the latter with good adherence capability of 987P fimbrial E.coli.

9仔猪小肠上皮细胞的黏附试验结果显示，30～35日龄M307~基因型和M307~基因型断奶仔猪的小肠上皮细胞均能与表达F18ab菌毛标准菌株107／86、诱导表达F18ac菌毛的重组大肠杆菌rE.coli1534及诱导菌体表面展示表达F18黏附亚单位FedF的重组大肠杆菌pnirBMisL-fedF发生黏附作用，而同日龄M307~基因型断奶仔猪的小肠上皮细胞均不能与上述三株大肠杆菌发生黏附作用。3日龄易感仔猪小肠上皮细胞也不能黏附上述三株大肠杆菌，但可以很好地黏附表达于987P菌毛的大肠杆菌。

After 4 weeks age, BALB/c mice were vaccinated with recombinant germ orally,got the gastrointestinal contents and inoculated culture and counted number at 2,6,12,24,48,72h,meanwhile got gaster, jejunum、ileum and appendix to cryostat section,then cryostat section wasput under the fluorescence microscope to observe the distribution of the recombinant germ.

分别取胃肠内容物接种培养并计数，同时取胃、空肠、回肠和盲肠做冷冻切片，在荧光显微镜下观察重组菌的分布。

The N gene ORF was then subcloned into pET-30a vector and the recombinant plasmid was transformed into E.coli BL21 (DE3) and induced with IPTG. The protein expression was determined by SDS-PAGE. The expressed protein had a molecular weight of 54.4 Ku that existed as inclusion body. Thin-layer scanning showed that the expression product accounted for 30.5% of the total bacterial proteins. The recombinant protein possessed native biological activity and could react with anti-PEDV hyperimmune serum in Western blot.

以阳性质粒为模板，用分别含有BamH Ⅰ和Xho Ⅰ酶切位点的上、下游引物扩增得到ORF，其PCR产物经BamH Ⅰ和Xho Ⅰ双酶切后定向克隆到pET-30a载体，构建的重组质粒命名为pET-30a-PN；将pET-30a-PN转化到大肠杆菌BL21 (DF3)中，在IPTG诱导下进行表达；SDS-PAGE结果表明表达出与预期大小相符的约54.4 Ku的重组蛋白，重组蛋白以包涵体形式存在；薄层扫描结果表明表达产物占菌体总蛋白的30.5%；Western blot分析表明表达的重组蛋白能与抗PEDV高免血清反应，说明该重组蛋白具有免疫学活性。

BMSCs and recombinant human GSF (50 μg/kg) were injected into models by tail vein and hypodermoclysis infusion respectively in the combination treatment group. Recombinant human GSF (50 μg/kg) was subcutaneously injected into models in the bone marrow stem cell autologous mobilization group. BMSCs were injected into rat models by tail vein in the BMSC group. Rat models in the heart failure group were intact.

造模后随机分成4组：联合治疗组尾静脉注射骨髓间充质细胞和皮下注入重组人粒细胞刺激因子50 μg/kg，自体骨髓干细胞动员组皮下注入重组人粒细胞刺激因子50 μg/kg，骨髓间充质干细胞组为尾静脉注射骨髓间充质细胞，心力衰竭组不给予任何干预。

Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势，并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。