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METHODS: Tum5 gene was amplified from plasmid pSPORT1Sfi by PCR technique and subcloned into the expression plasmid of lentiviral vector, pGCFU, to generate the lentiviral expression vector, pGCFUTum5. The correct Tum5 gene was confirmed by endoenzyme digestion and sequencing. Recombinant lentiviruses were produced by 293T cells following the cotransfection of pGCFU Tum5 and packaging plasmids-pHelper1.0and pHelper2.0. The resulting recombinant lentiviruses (GCFUTum5) which carried Tum5 and EGFPgene were then used to infect human umbilical vein endothelial cells.

采用PCR技术从含有tumstatin基因的质粒克隆模板 pSPORT1Sfi钓取Tum5基因，并将基因克隆到慢病毒载体表达质粒pGCFU中，构建慢病毒载体表达质粒pGCFUTum5，通过酶切、测序验证Tum5基因后，将pGCFUTum5质粒和包装质粒pHelper1.0，pHelper2.0共同转染人胚胎肾上皮细胞系293T细胞，获得携带Tum5基因和EGFP基因的重组慢病毒GCFUTum5，并转染靶细胞人脐静脉血管内皮细胞。

Recombinant mouse enterokinase can be expressed in Ecoli in inclusion manner so that the prokaryotic expression system for recombinant enterokinase with native conformation needs to be optimized.

鼠源性肠激酶轻链在原核细胞中多以包涵体形式表达，天然构象重组肠激酶的获得还需对表达系统进行优化。

After thermal induction, no specific recombinant protein band in SDS-PAGE was found, but G-CSF activity was detectable. Therefore, a new recombinant plasmid pBVHG2 expressing hG-CSF hybrid protein with additional 8 amino acids which could be cut off specifically with the help of mucosal enterokinase at the N terminus of hG-CSF was constructed.

含此质粒菌株虽然表达菌体裂解后可测得明显的生物学活性，但SDS-PAGE仍未见特异表达产物带；因此，再应用相同方法，在hG-CSF cDNA突变体5′端增加24核苷酸对的FLAG肽编码序列，构建了hG-CSF杂交蛋白（hG-CSF天然蛋白N末端增加8aa的FLAG肽，后者可由肠激肽酶切除）的表达质粒pBVHG2。

Methods The LPL cDNA was isolated from the human epiploon adipose tissue by means of RT-PCR. The LPL cDNA was ligated into the pcDNA3.1Zeo. The recombinant pcDNA3.1Zeo-LPL cDNA was identified by endonucleases, PCR and DNA sequencing. COS-1 cells were transfected with the recombinant LPL gene plasmid using Lipofectamine 20001M The LPL mass in cells and the culture medium were determined by a Markit-M LPL Kit. Spectrophotometry was used to measure the LPL activity.

采用RT-PCR法从人大网膜脂肪组织获取LPL cDNA基因，以pcDNA3.1Zeo质粒作为载体，运用基因克隆技术构建野生型人LPL cDNA重组质粒，限制性内切酶酶切、PCR以及双向测序鉴定重组质粒；运用Lipofectamine 2000将重组pcDNA3.1 Zeo-LPL cDNA质粒导入COS-1细胞，RT-PCR法检测LPL mRNA，ELISA法和比色法分别检测细胞裂解液及细胞培养基中LPL浓度及活性。

Recombinant Human Osteogenic Protein (rhOP-1) provides a strong arm to solve this difficult problem. The rhOP-1 is produced by DNA recombinant technique, with the characteristics of high purity, high output, low cost and high osteoinductive activity. How does OP-1 work on the erective spinal fusion?It has not been documented yet.

重组人OP-1（rhOP-1）的出现为这个问题的解决提供了一个强有力的武器。rhOP-1是通过DNA重组技术生产，具有纯度高、产量高、成本低等优点，具有高效的诱导成骨活性。rhOP-1在直立脊柱融合术中的作用如何，目前尚未见文献报道。

In this study, a recombinant plasmid generating short hairpin RNA was constructed — To construct the recombinant plasmid carrying shRNA to HPV6bL1 and analyze the nucleicacid sequence for further searching new gene therapy method of cauliflower excrescence.

目前对于尖锐湿疣仍无有效的病毒特异性防治措施来保护人类免受其危害，而基因疫苗则为预防HPV感染提供了可能性；L1是HPV6型主要的衣壳蛋白，具有较强的免疫原性，可刺激机体产生保护性的中和抗体。

The 528 bp fragment of flic gene was successfully cloned into vector, the pGEX-flic recombinant plasmid was expressed and induced by IPTG and Western-blot method showed good antigenicity of the recombinant protein, and there were flexible regions such as coil region and turn region in flic protein.

获得的目的基因片段长度为528 bp，测序表明其序列与GenBank公布的一致；软件预测结果显示，该蛋白具有多个明显的二级结构成分及跨膜区；SDS-PAGE检测表明，在约50 ku处有一特异性条带；Western-blot检测表明，表达蛋白具有良好的抗原活性。

The flic gene of App was obtained by PCR based on highly homology with Escherichia coli, Salmonella, Shigellosis. Then it was legated to pMD-18T vector. The recombinant plasmid was identified by enzyme and sequence analysis. The 528 bp fragment of flic gene was successfully cloned into pGEX-KG vector, then the recombinant plasmid was transformed into E.

以猪传染性胸膜肺炎放线杆菌血清Ⅰ型菌株基因组为模板，根据其鞭毛区与其它细菌高度同源性设计引物，利用PCR方法扩增鞭毛蛋白基因flic片段，将其亚克隆到pMD-18T载体中并进行PCR和酶切鉴定，再将其亚克隆与载体pGEx-kG分别双酶切和连接，构建重组表达载体pGEX-flic，并将其转化大肠杆菌工程菌BL21，进行原核表达。

Methods The recombinant fowl pox virus transfer vector pSY681-gD-LacZ carrying gD gene of ILTV HNCG strain was transfected to chick embryo fibroblast infected with FPV-017 strain, then recombinant FPV was purified by blue plaque screening and identified by PCR and indirect IFA.

将含有ILTV河南长葛株gD基因的禽痘病毒转移载体转染禽痘病毒感染的鸡胚成纤维细胞，采用蓝色蚀斑筛选纯化重组禽痘病毒，并经PCR及间接免疫荧光试验进行鉴定。

Objective: In order to investigate whether the recombinant adeno-associated virus -mediated foreign genes, LacZ/hVEGF, could pass the blood brain barrier by intra-carotid artery delivery or not and to investigate whether the foreign genes could be expressed in vivo in ischemic brain of the focal embolic stroke rats or not, a high titer and completely adenovirus free recombinant adeno-associated virus vector was constructed and studied.

目的：为了检测腺相关病毒载体携带的人类血管内皮因子基因能否通过血脑屏障及能否在脑组织中表达其基因产物，我们构建了一种新型、高滴度、无腺病毒污染的rAAV携带的报告基因和hVEGF基因分别经颈动脉对自体血栓局部脑卒中大鼠进行了研究。

Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势，并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。