查询词典 reciprocal fundamental vectors

The lysis efficiency was about 90% for heat-inducible vector or around 30% forUV inducible vectors. This set of autolytic units should be transferable to other vectors.

本体系能够通过简单的物理方法，将具有生物活性的胞内酶释放到胞外的，解决了定向进化的一个瓶颈问题。

Objective To construct retrovirus vectors carrying IL17 or siRNAIL17 genes with Thy1.1 gene and determine the infected ability of the retrovirus vectors to diabetogenic BDC2.5 T cells.

目的 构建含有Thy1.1细胞表面抗原(Thy1.1 cell surface antigen，Thy1.1)基因的携带IL17或siRNAIL17基因的逆转录病毒载体，并观察逆转录病毒对致糖尿病性BDC2.5 T细胞的转染能力。

Methods The NGF, BDNF, and NT3 genes of rats were cloned, the eukaryote expression vectors were established, the three kind of recombinant vectors were used to transfect astrocytes, the positive cloned cells were cultured dilatedly after G418 sifting; using supernates of culture liquid of astrocytes modified by gene to culture PC12 or TrkB-PC12, the expression and its level of gene target cells aimed genes were measured by Western blotting or immunohistochemical method.

克隆大鼠NGF、BDNF和NT3基因，构建真核表达载体；3种重组载体分别转染星形胶质细胞，G418筛选后获得阳性克隆细胞进行扩大培养；取基因修饰星形胶质细胞培养液上清培养PC12细胞或TrkB-PC12细胞；用Western blotting杂交或免疫组织化学方法检测基因靶细胞目的基因的表达及其水平。

This review focuses on the development and means of the modification of polymeric gene vectors making them have multifunction, i.e. the ability to maintain stability outside of cell, the ability of cell entrance, endosome escape, and the ability of nuclear membrane traversing. The main challenges remain and the future direction of polymeric vectors research are discussed.

本文在分析基因治疗过程的基础上，详述了基因载体应具有的4种基本功能－在细胞外保持稳定的功能、进入细胞的功能、逃离溶酶体的功能和进入细胞核的功能，并详细介绍了有关基因载体功能化修饰的方法和目前国际上的进展情况，总结了高聚物载体目前存在的主要问题及未来的发展方向。

Some methods aiming at the two problems have been researched. First, the optimal number of ATE site can be calculated based on a cost model of DAT-ATE. Second, the problem of test schedule which is equitant with two-dimensional Bin-Packing problem is presented. Then a TCG denotation satisfied with T-admissible rule is more convenient for combinational optimization. With Simulated annealing algorithm, better test scheduling results can be achieved; but there is no great optimization space because the efficiency of scheduling is greater than 90%. Third, A novel test compression method——Multi-capture testing is proposed to compress the stimuli. And the ATPG results show that MC achieves high compression ratio which is greater than 90% in some large-scale circuits. MISR, widely used in LBIST, is employed to compress the responses. The aliasing analysis demonstrates that the fault coverage reduction is little due to the existence of aliasing. Fourth, an improved TIC (called S-TIC) aiming at structured test is proposed based on ARM's TIC. A MC scheduling algorithm is proposed to compute the SoC test time and combine MC vectors to SoC test vectors. The scheduling results shows that test time is greatly reduced when using the proper scheduling priority.

首先，给出了低成本ATE的成本模型，根据此模型得到最低测试成本时系统级测试调度的基本参数——测试Site数；其次，本文将DAT-Scan方式的SoC测试调度等价为两维BP问题，为有效地求解该问题，将该BP问题表示为TCG图并通过模拟退火算法解决其求解过于复杂的问题，调度的结果表明该方法在测试时间上有一定改善，并指出DAT-Scan测试调度效率已经大于90％，进一步优化的空间较小；第三，本文尝试通过测试压缩来解决激励所需的测试带宽，提出了Multi-capture结构并解决了MC测试过程中的&矢量&退化问题，MC测试的ATPG结果表明其测试压缩率很高（大电路接近90％）；第四，在激励获得很高压缩比的情况下，测试响应也需压缩，本文采用LBIST中常用的MISR作为MC测试的响应压缩电路，理论分析和实验结果都证明了MC测试的别名对故障覆盖率影响较小（小于2％），并对两类别名的成因做了具体分析；第五，在MC测试及其响应压缩的基础上，本文改进了ARM公司的测试控制器TIC使之适合于MC测试，为了解决MC测试矢量合成问题，本文抽取了MC测试模型，通过固定优先级的MC测试调度模拟将MC矢量合成为ATE矢量，并模拟出了总的MC测试时间。

Different plant expression vectors had been respectively transferred into upland cotton cultivars Jinman12, Jinmian7, Xinluzao1 and Jihe321 via Agrobacterium tumfaciens transformation. These vectors carried cryIAc3 gene, which encodes Bacillus thuringiensis δ-endotoxin, under the control of chimeric OM promoter, CaMV 35S promoter, and cotton leaf curl virus RP promoter respectively; snowdrop lectin gene (Galanthus nivals agglutinin, gna) trans-regulated by rolC promoter controlled TGAla factor; and multivalent expression construct of both cryIAc3-cpti fusion gene and gna gene. A large number of transgenic plants and their progenies had been obtained.

为了提高外源基因的表达量，延缓害虫产生耐受性，本文通过将携带有分别在高效复合OM启动子，CaMV35S启动子及棉花曲叶病毒RP启动子控制下的苏云金杆菌杀虫毒蛋白基因cryIAc3；反式调节因子增强韧皮部表达的雪花莲外源凝集素(Galanthus nivals agglutinin，GNA)基因；以及同时含有cryIAc3、豇豆胰蛋白酶抑制剂基因和gna三价抗虫基因的植物表达载体，以根农杆菌介导法分别将这些表达载体导入了陆地棉新陆早1号、晋棉7号、晋棉12号和冀合321等我国西北棉花主要栽培品种，经体细胞胚再生获得了大批转基因再生植株。

Parkinson s disease is a degenerative disease of the basal ganglia causing tremor at rest, muscle rigidity and hypokinesia Gene therapy is most likely to cure PD completely, and the most promising one among all the treating ways But its effectiveness and safety have a strong relationship with the vectors chosed This article introduces some usually used virus vectors and their characteristics as well as the trend of virus vector in PD s gene therapy

帕金森病是一种神经节退行性疾病，主要以静止震颤，肌肉僵直和运动减少为临床症状。在其各种治疗方法中基因治疗最有望彻底治愈PD，最有发展前景。但其有效性与安全性与其所选用的载体有很大关系。本文介绍了基因治疗中常用的病毒载体以及它们的特性，并指出PD基因治疗中病毒载体的发展趋势。

The signature vectors are estimated by projection onto the intersectant subspaces. Based on the estimated signature vectors, the multiuser detector is constructed.

利用到相交子空间的迭代投影估计得到包含信道信息的特征矢量，以此构造多用户接收机。

The protozoa were isolated from field animal blood, vectors, pathogenic tissue and secretions. Based on differences of the pathogen morphology, vector species, experimental infected animals, single parasite clone and parasitic tissues, protozoiasis pathogens from different areas and animals in China were isolated and the vectors and transmission mode were also determined by vector transmission trial. Sequence analysis of 18S rRNA gene was used to study the phylogeny of partial isolated protozoa.

自野外采集动物血液、传播媒介、相关组织、排泄物等，根据对病原形态鉴定、传播媒介鉴定、实验性感染易感动物、单一虫株克隆、不同致病部位等方法，对我国不同地区、不同家畜的原虫病病原进行分离，并通过媒介传播试验确定传播媒介的种类与传播方式；采用18S rRNA基因测序方法，对本实验室已分离的部分原虫进行进化关系研究。

In this algorithm,by constructing a UserlD-URL revelant matrix similar customer groups are discovered by measuring similarity between column vectors and relevant web pages are obtained by measuring similarity between row vectors;frequent access paths can also be discovered by further processing of the latter.

第四是本文将传统数据挖掘过程中的各种关键技术，引入到对Web使用信息的挖掘活动中，结合关系数据库的特点设计并实现了一个具有可广西人学颀士学位论义视化功能的Web使用挖掘系统WLGMS。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。