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Methods Peripheral blood neutrophils were islolated from health men, and then cultured in DMEM medium containing 10% fetal calf serum for 3 h. Blank medium or gonococcal inocula was added into group A or group B. NO concentration and iNOS mRNA expression at 0, 3, 8 and 12 h were determined by cadmium reduction method and realtime quantitative fluorescent PCR, respectively.

分离正常人外周血中性粒细胞，在10%胎牛血清DMEM培养液上培养3 h，A组加入空白培养基，B组加入淋球菌菌液，随后在0、3、8、12 h分别以实时定量荧光PCR测定iNOS mRNA表达，用镉还原法检测NO浓度。

Methods Realtime fluorescence quantitive RTPCR was used to detect the expression level of Tiam1 mRNA in gastric cancer tissues of 66 cases, paracancerous tissues (over 3cm) of 66 cases and gastric benign lesion tissues of 11 cases. Meanwhile, CK18 was selected as the hallmarker for lymph node micrometastasis to detect the level of CK18 mRNA expression in 199 lymph nodes from 24 cases of gastric carcinomas diagnosed negative by pathologic examinations.

采用TaqMan探针实时荧光定量PCR方法，定量检测66例胃癌患者癌组织、癌旁组织及11例胃良性病变组织中的Tiam1 mRNA拷贝数，同时将CK18作为淋巴结微转移的标志基因，定量检测24例病理诊断无淋巴结转移的胃癌患者199枚淋巴结的CK18mRNA拷贝数。

More realtime visual and haptic interaction with anatomical data ［J］.

适用于CT薄层扫描和三维重建肝脏管道系统的灌注和铸型的建模研究［J］。

Objective: To investigate the characteristic imaging and diagnostic value of infantile hypertrophic pyloric stenosis by realtime ultrasonography.

目的：探讨实时超声对新生儿肥厚性幽门梗阻的诊断特点及应用价值。

Methods: Fifteen realtime ultrasonography performed for possible infantile hypertrophic pyloric stenosis were analyzed retrospectively.

采用高频探头对15例疑为幽门梗阻的新生儿及婴儿患者的幽门部的实时超声图像进行分析，所有患者均经手术病理证实。

As of a high performance realtime image processing system, the hardware model and software design of the image data grabber are important samely.

海量图像数据的实时采集是进一步实时图像处理的基础。对一个高性能实时图像处理系统来说，采集卡的硬件模型与软件设计同等重要。

Based on the research of Realtime Management-Control Integration Teconology,we propose a real-time management-control integration application system for bulk transportation in port,and give an introduction to an application case in Xiji Stevedoring Branch Company of Guangzhou Port Group Co.

在研究实时管控一体化技术的基础上，针对港口散货运输业务模式提出适合我国港口的实时管控一体化解决方案，并介绍广州港集团有限公司西基港务分公司应用实例。

Summary: A new resolution of multichannel synchronal data acquisition which is realtime and based on FPGA is introduced.The hardware structure and control logic are described emphatically,moreover,the rules of circuit implement are also given.

介绍了一种基于FPGA的多路同步、实时数据采集新方案，着重对其硬件结构和控制逻辑进行了阐述，并从工程实践方面给出了电路的实现原则。

The resolution,which is flexible,expandable and general,is successfully introduced into the design of system of dualmodule data fusion to implement realtime synchronal data acquisition for radar seekers.

该方案控制方式灵活可变、具有一定的扩展性和通用性，已被成功地应用于双模信息融合系统的设计中，实现了对雷达导引头的多信号实时同步采集。

No game is released by Realtime Gaming until it has been certified by TST.

没有游戏发布实时游戏，直到它已被尖沙咀认证。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。