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In mouse peritoneal macrophages. Methods Mouse peritoneal macrophages were cultured in the presence of heat-killed yeast-phase PM for 24 h, and the average fluorescence intensity of TLR-2, TLR-4, and Dectin-1 in the macrophages was detected using flow cytometry. Fluorescent staining of the macrophages was performed to observe the fluorescence of TLR-2, TLR-4, and Dectin-1 with confocal microscopy. TNF-α mRNA in the cell culture supernatant was measured with real-time PCR, and TNF-α protein detected using enzyme-linked immunosorbent assay.

马尔尼菲青霉酵母相菌液与小鼠腹腔巨噬细胞共培养24h，采用流式细胞技术检测巨噬细胞TLR-2、TLR-4及Dectin-1的平均荧光强度；共聚焦显微镜观察荧光染色的受体；ELISA法测定培养液上清中TNF-α的浓度；Real time PCR检测不同时间段TNF-α的mRNA表达。

The established event-specific real-time PCR detection systems in this study are suitable for the identification and quantification of these GM maizes.

再辅以即时聚合酶链锁反应( real-time PCR )检测基因改造玉米含量，将有助於建立基因改造玉米产品标示管理。

With us in England, till modern times, a man could only dispose of one third of his moveables from his wife and children: and, in general, no will was permitted of lands till the reign of Henry the eighth; and then only of a certain portion: for it was not till after the restoration that the power of devising real property became so universal as at present.

在我们英格兰，直到现代，一个人仅仅能够随意处置留给他妻子和孩子的动产的三分之一：并且一般的，不允许随意处置土地的情况一直到亨瑞八世才废止；之后也只能处置一部分土地：因为直到查理二世恢复原有秩序之后遗赠真实财产（real property：不动产，包括土地及居所。

The fourth time is the charm for the always positive, always uplifting Sanctus Real. We Need Each Other is more than just the band's best release; it is one of the most melodic Christian pop albums to come along in quite some time.

这是一直乐观向上的基督流行摇滚乐团 Sanctus Real 的第四次惊艳登场；&We Need Each Other&不仅是作为乐队的已发行的最好的一张专辑，而且是这段时间最棒的旋律基督流行专辑之一。

As an excellent game developer in China, Tose China has been developing almost 300titles. We develop games in various platforms and we run a good relationship with a lot of famous game development companies inside and outside China. For example, we are one of the top5 worldwide outsourcing developer for Electronic Arts Inc and we are also the long-term cooperator for The9 China, Ourgames China and REAL China.We are keeping a long-term relationship with NINTENDO,SEGA,BANDAI NAMCO,CAPCOM,SQUARE ENIX,TAITO as well.

在中国作为游戏行业中业绩卓著的东星中国已经开发了近300款游戏，并涉及各种游戏平台进行开发，与众多的国内外顶级知名游戏公司均保持良好的合作关系，例如我们是美国的Electronic Arts Inc全球5大合作外包企业之一，是国内的第九城市，联众，REAL的长期合作伙伴，并和日本的任天堂，世嘉，万代南梦宫，CAPCOM，SQUARE ENIX，TAITO等均保持着长期的项目合作。

The results of his labor and dedication can be heard on seven titles for Real Music, Beyond the Horizon, Feather Light, Dream Spiral, The Edge of Forever, Winter Magic, Sweet Return and finally, Hilary Stagg ~ A Tribute.

他辛苦和奉献的成果可以Real Music的七个主题中体现出来，超越了界限，轻柔明亮，虚幻曲折，接近永恒，神秘的冬季，甜蜜的回归和Hilary Stagg 的礼物。

However, RFID stands for Radio Frequency Identification, and is also called as Electronic Label. It goes through radio signals to identify specific targets, and reading and writing information, as well as don't need identity system and specific targets to create such as electronic, optics or machinery. Properties of wireless communication, space-penetrating, information tracking and real-time location system/ RTLS will apply for increasing the system capability of safety and protection to Robot.

而「RFID， Radio Frequency Identification 」称之为「无线射频识别」，又称「电子标签」，是一种通信技术，可透过无线电讯号识别特定目标并读写相关资料，而无需识别系统与特定目标即可建立含电子、光学或机械接触，其特性：无线通讯、空间穿透性、资讯历程可追踪与「即时定位系统 RTLS， Real Time Location System」等，必然将成为机器人、机械手臂安全设计与防护的新应用。

It's a very beautiful house near by El Camino Real. And there are duck everywhere. What exactly this house is for ??

El Camino Real上不知名的建筑，不晓得是干麻的，到处都是野鸭子，不过挺美的

In 1998, 29 people were killed by a car bomb in Omagh, Northern Ireland; a splinter group calling itself the Real I-R-A claimed responsibility.

1998年，在英国北爱尔兰西部城镇奥马，有29人被汽车所投的炸弹炸死：*示威者要求他们的the Real I-R-A（英国的一个部门，但是我查不到）对其事件负责。

Methods:The difference of PTH gene and protein expression in parathyroid gland and thymus were compared between PTH knock-out mice 1αase knock-out mice and their wild-type littermates at 2 weeks of ages by RT-PCR,real-time PCR and immunohistohemistry.

real-time PCR和免疫组织化学的方法比较分析同窝2周龄PTH基因敲除纯合子和野生型小鼠以及1-α羟化酶基因敲除纯合子[1αase-/-]和野生型小鼠的甲状旁腺和胸腺的PTH表达差异？

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐